Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	192.5 / NA	56	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (23/33);  total (23/33)

CGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193708‑2193851                                                                        |                                                                         cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCCATAACAGCAAAACGCCAATcccc                                                                      >  2:1277424/1‑76 (MQ=255) cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcccc                                                                      >  2:1635305/1‑76 (MQ=255) cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcccc                                                                      >  2:612218/1‑76 (MQ=255) cGATGCATCCGGCGTCCAGGCGCGGCTGGCCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAAAGCCAATcccc                                                                      >  1:1848909/1‑76 (MQ=255)  gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCCATAACAGCAAAAAGCCAATCCcca                                                                     >  1:1056622/1‑76 (MQ=255)  gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                                                                     >  2:1307882/1‑76 (MQ=255)  gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                                                                     <  1:1692474/76‑1 (MQ=255)  gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                                                                     <  1:252191/76‑1 (MQ=255)   aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccac                                                                    >  1:1574668/1‑76 (MQ=255)     gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacca                                                                  >  2:412717/1‑76 (MQ=255)     gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacca                                                                  <  2:1200622/76‑1 (MQ=255)      cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAg                                                                 <  1:1301508/76‑1 (MQ=255)       aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGa                                                                >  1:657429/1‑76 (MQ=255)       aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAACCCCCCCAGa                                                                >  1:1498606/1‑76 (MQ=255)        tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCCCCAGAt                                                               >  1:1330520/1‑76 (MQ=255)           ggCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa                                                            <  1:1742434/76‑1 (MQ=255)             cGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCCATAACAGCAAAACGCCAATCCCCACCAGATAAAGc                                                          >  2:818777/1‑76 (MQ=255)             cGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGc                                                          <  1:536059/76‑1 (MQ=255)              gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa                                                         <  1:174875/76‑1 (MQ=255)              gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa                                                         <  1:825847/76‑1 (MQ=255)                      gcgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt                                                 >  2:1676080/1‑76 (MQ=255)                      gcgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt                                                 >  2:75649/1‑76 (MQ=255)                       cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                                <  2:177888/76‑1 (MQ=255)                         gCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGtn                                              <  1:992506/76‑2 (MQ=255)                          cTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             <  2:1617665/76‑1 (MQ=255)                              tCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCCGATAAAGCATGACGCCTGTATTaaa                                         >  2:83278/1‑76 (MQ=255)                              tCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         <  1:851863/76‑1 (MQ=255)                                 ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                      >  1:232789/1‑76 (MQ=255)                                     gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  >  2:639261/1‑76 (MQ=255)                                     gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:1868304/76‑1 (MQ=255)                                     gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  1:217797/76‑1 (MQ=255)                                        ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  2:6730/76‑1 (MQ=255)                                         gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              >  1:923858/1‑76 (MQ=255)                                         gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  2:529123/76‑1 (MQ=255)                                         gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  1:475548/76‑1 (MQ=255)                                         gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  1:202725/76‑1 (MQ=255)                                          aaaTCAGCCCCAATAACAGCCAAACGCCCATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             <  2:17159/76‑1 (MQ=255)                                          aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             >  2:753010/1‑76 (MQ=255)                                          aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             <  1:329626/76‑1 (MQ=255)                                             tCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa                          <  1:1171371/76‑1 (MQ=255)                                              cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         <  2:1711687/76‑1 (MQ=255)                                              cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         >  1:1193620/1‑76 (MQ=255)                                              cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         <  2:27703/76‑1 (MQ=255)                                                gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg                       <  2:351107/76‑1 (MQ=255)                                                       tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGTGCATcg                <  1:1277424/76‑1 (MQ=255)                                                       tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                >  2:962499/1‑76 (MQ=255)                                                       tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                <  1:1635305/76‑1 (MQ=255)                                                       tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                <  1:568559/76‑1 (MQ=255)                                                       tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTCACCGATGGCGCATcg                <  1:612218/76‑1 (MQ=255)                                                          cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             <  2:1210208/76‑1 (MQ=255)                                                            gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc           <  2:1515684/76‑1 (MQ=255)                                                              aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  2:652587/76‑1 (MQ=255)                                                               aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg        <  2:1546797/76‑1 (MQ=255)                                                                aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGTCGCGTCGGGCACCGgc       >  2:106077/1‑76 (MQ=255)                                                                aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       >  2:1242054/1‑76 (MQ=255)                                                                aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       >  2:1254173/1‑76 (MQ=255)                                                                  cGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccg     >  2:1629472/1‑76 (MQ=255)                                                                     cAATCCCCACCAGATGAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  <  2:765887/76‑1 (MQ=255)                                                                     cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  1:677606/1‑76 (MQ=255)                                                                     cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  1:946413/1‑76 (MQ=255)                                                                     cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  2:1842984/1‑76 (MQ=255)                                                                        |                                                                         CGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193708‑2193851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑