| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,883,147 | (A)5→4 | intergenic (‑341/+276) | atpI ← / ← gidB | F0F1 ATP synthase subunit I/glucose‑inhibited division protein; b3740_1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,883,143 | 0 | A | . | 92.3% | 218.2 / 13.8 | 52 | intergenic (‑337/+280) | atpI/gidB | F0F1 ATP synthase subunit I/glucose‑inhibited division protein; b3740_1 |
| Reads supporting (aligned to +/- strand): ref base A (3/1); new base . (26/22); total (29/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
TGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGT > REL606/3883070‑3883212 | tGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTaaa < 1:1547583/76‑1 (MQ=255)tGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTaaa < 1:1569436/76‑1 (MQ=255) gcgcATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTaaaa < 2:1080119/76‑1 (MQ=255) gcgcATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTaaaa < 2:868577/76‑1 (MQ=255) cgcATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTaaaac > 2:1155066/1‑75 (MQ=255) gcATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTaaaaca > 1:1415053/1‑74 (MQ=255) cATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTaaaacag < 1:1487637/76‑4 (MQ=255) cATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTaaaacag < 1:640394/76‑4 (MQ=255) tGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGAt > 2:450461/1‑76 (MQ=255) gTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATa < 2:1539687/76‑1 (MQ=255) gTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATa < 1:72450/76‑1 (MQ=255) tCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACt > 2:145553/1‑76 (MQ=255) cgcACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGtt > 1:1239404/1‑76 (MQ=255) cacaGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGtttt < 2:1010796/76‑1 (MQ=255) cacaGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGtttt < 2:1212080/76‑1 (MQ=255) acaGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTta < 2:336389/76‑1 (MQ=255) caGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTtaa < 2:152757/76‑1 (MQ=255) aGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTtaat < 1:178931/76‑1 (MQ=255) cATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTAAAAACAGATACTGTTTtaata < 2:915993/76‑1 (MQ=255) tatttagtTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAAt < 2:1097823/76‑1 (MQ=255) tttaCTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACAtt < 2:665008/76‑1 (MQ=255) ttaCTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACAttt < 1:571163/76‑1 (MQ=255) ttaCTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACAttt > 1:1687005/1‑76 (MQ=255) ttaCTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACAttt > 2:1032944/1‑76 (MQ=255) tGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTacac > 2:316406/1‑76 (MQ=255) tGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTAcaca > 1:1707466/1‑76 (MQ=255) tGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTAcaca > 2:1180186/1‑76 (MQ=255) gCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACacaac > 2:434528/1‑76 (MQ=255) cAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACacaaca > 2:1728930/1‑76 (MQ=255) cAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACacaaca > 2:960927/1‑76 (MQ=255) aaTGATGCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACaaa < 1:1113453/76‑1 (MQ=255) atgatgCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACaaaa < 2:1527101/76‑1 (MQ=255) tgatgCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACaaaaa > 1:1047281/1‑76 (MQ=255) tgatgCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACaaaaa < 2:546523/76‑1 (MQ=255) atgCCTTTGCAAGTTTATGATATTTCAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAac > 2:34089/1‑76 (MQ=255) atgCCTTTGCAAGTTTATGATATTTCAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAac > 2:34057/1‑76 (MQ=255) atgCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAacc > 1:661246/1‑76 (MQ=255) atgCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAacc < 1:698348/76‑1 (MQ=255) gCCTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAaccac > 2:1671925/1‑76 (MQ=255) ccTTTGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAaccacc > 1:1170920/1‑76 (MQ=255) tttGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCa < 1:99860/76‑1 (MQ=255) ttGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCAt > 1:1365291/1‑76 (MQ=255) tGCAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCAtt > 1:760012/1‑76 (MQ=255) cAAGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGa < 2:988549/76‑1 (MQ=255) aGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACa > 1:83244/1‑76 (MQ=255) aGTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACa < 2:946244/76‑1 (MQ=255) gTTTATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACAt < 2:1447407/76‑1 (MQ=255) tttATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACAtt > 2:567313/1‑76 (MQ=255) tttATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACAtt > 2:82667/1‑76 (MQ=255) ttATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACAttt > 2:609120/1‑76 (MQ=255) ttATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACAttt > 1:1680401/1‑76 (MQ=255) ttATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACAttt > 1:335989/1‑76 (MQ=255) tATGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACAtttt > 2:723155/1‑76 (MQ=255) aTGATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACAttttt < 1:1216381/76‑1 (MQ=255) gATATTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTtaa < 2:1137478/76‑1 (MQ=255) atatTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTtaat > 2:1640578/1‑76 (MQ=255) tatTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTtaata > 1:175394/1‑76 (MQ=255) tatTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTtaata < 2:768263/76‑1 (MQ=255) tatTTCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTtaata > 2:101075/1‑76 (MQ=255) tCAGTCT‑AAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGt < 2:63116/76‑1 (MQ=255) | TGCGCATGTTCGCGCACAGCATATTTATTTACTTGGCAAATGATGCCTTTGCAAGTTTATGATATTTCAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGT > REL606/3883070‑3883212 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |