Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	474,807	Δ1 bp	coding (499/1677 nt)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	474,806	0	A	.	100.0%	202.1 / NA	41	coding (500/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (26/15);  total (26/15)

GATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTG  >  REL606/474733‑474877                                                                          |                                                                        gATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGgcagcaca                                                                       >  2:657085/1‑74 (MQ=255) gATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGgcagcaca                                                                       >  1:1320151/1‑74 (MQ=255)  aTCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGgcagcacac                                                                      <  1:1102867/76‑4 (MQ=255)    ccccATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCa                                                                   >  2:378480/1‑76 (MQ=255)            cGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGa                                                           <  1:113970/76‑1 (MQ=255)            cGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGa                                                           >  2:1009168/1‑76 (MQ=255)             gccTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGaa                                                          <  2:1287139/74‑1 (MQ=255)             gACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGaa                                                          >  1:1386148/1‑76 (MQ=255)                tGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCa                                                       >  1:227448/1‑76 (MQ=255)                tGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCa                                                       <  1:368191/76‑1 (MQ=255)                 gCAGGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCAt                                                      <  1:71843/76‑1 (MQ=255)                 gCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCAt                                                      >  2:855486/1‑76 (MQ=255)                 gCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCAt                                                      <  1:867400/76‑1 (MQ=255)                   aaGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTa                                                    >  1:606779/1‑76 (MQ=255)                   aaGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTa                                                    >  2:1086543/1‑76 (MQ=255)                     gagTGGCAAACCCCACATCGCCCTTTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTAcc                                                  <  1:1743600/76‑1 (MQ=255)                       gTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAg                                                >  2:501477/1‑76 (MQ=255)                       gTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAg                                                <  1:1419243/76‑1 (MQ=255)                       gTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAg                                                <  1:1203378/76‑1 (MQ=255)                       gTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAg                                                >  2:874259/1‑76 (MQ=255)                       gTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAg                                                >  1:1165435/1‑76 (MQ=255)                        tGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAgg                                               >  1:1426380/1‑76 (MQ=255)                         ggCAAACCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAGGt                                              <  2:1586253/76‑1 (MQ=255)                             acgCCCACCTCCCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAGGTCTTc                                          <  2:1220624/74‑1 (MQ=255)                             aaGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAGGTCTTc                                          >  2:488921/1‑76 (MQ=255)                               gCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAGGTCTTCca                                        >  1:1100089/1‑76 (MQ=255)                               ccccACATCGCCCTGTCCCATCATCCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAGGTCTTCca                                        <  1:880561/75‑1 (MQ=255)                                cccACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAGGTCTTCcac                                       >  1:1018295/1‑76 (MQ=255)                                 ccACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAGGTCTTCcaca                                      >  1:148553/1‑76 (MQ=255)                                 ccACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAGGTCTTCcaca                                      >  2:1532437/1‑76 (MQ=255)                                 ccACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAGGTCTTCcaca                                      >  1:1612650/1‑76 (MQ=255)                                       cGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATcaac                                >  2:631221/1‑76 (MQ=255)                                          ccTGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATcaaccaa                             <  2:412933/76‑1 (MQ=255)                                            tGTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCaaccaacc                           >  2:756144/1‑76 (MQ=255)                                             gTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCg                          <  2:1071912/76‑1 (MQ=255)                                             gTTCCATCATTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCg                          >  1:813340/1‑76 (MQ=255)                                                   tcatTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCg                    >  1:875585/1‑76 (MQ=255)                                                    catTCCTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGa                   >  2:420188/1‑76 (MQ=255)                                                     atcccTGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGAt                  <  1:843275/73‑1 (MQ=255)                                                         ntGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGc              >  1:106536/2‑76 (MQ=255)                                                          tGCCACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCACCCAACCGATGGCGATTTGcc             >  1:816551/1‑76 (MQ=255)                                                            ccACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCa           >  2:1655405/1‑76 (MQ=255)                                                             cACTGCGGGCAGC‑ACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCAc          <  1:439152/76‑1 (MQ=255)                                                                   gggCAGC‑ACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGAc    >  1:1231689/1‑76 (MQ=255)                                                                     gcagc‑aCACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTg  <  1:1181869/76‑1 (MQ=255)                                                                          |                                                                        GATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTG  >  REL606/474733‑474877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑