| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | REL606 | 3,014,094 | (ACAGGC)3→2 | coding (675‑680/1137 nt) | ECB_02815 → | Capsule polysaccharide export inner‑membrane protein kpsE |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | REL606 | 3014087 = | 1 (0.020) | 43 (0.940) | 34/124 | 0.3 | 97.9% | coding (668/1137 nt) | ECB_02815 | Capsule polysaccharide export inner‑membrane protein kpsE |
| ? | REL606 | = 3014080 | 1 (0.020) | coding (661/1137 nt) | ECB_02815 | Capsule polysaccharide export inner‑membrane protein kpsE | |||||
AAGGCACGCCAGCGTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATACGCTGATGGGCCAGAAGATCCAGATGGAAGCGGAC > REL606/3014008‑3014154 | agggcaCGCCAGCGTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcac > 1:917913‑M2/3‑73 (MQ=255) aCGCCAGCGTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACACCAACGTTCTGGATCcacaggca > 2:1604732‑M2/1‑68 (MQ=255) cGCCAGCGTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcac > 1:990087‑M2/1‑67 (MQ=255) gCCAGCGTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcaca < 2:366825‑M2/76‑11 (MQ=255) ccAGCGTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcacag < 1:596194‑M2/76‑12 (MQ=255) cAGCGTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcacagg > 2:148259‑M2/1‑64 (MQ=255) aGCGTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcacaggc < 2:939359‑M2/76‑14 (MQ=255) aGCGTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcacaggc > 2:892035‑M2/1‑63 (MQ=255) aGCGTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcacaggc < 2:1009725‑M2/76‑14 (MQ=255) gCGTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcacaggcg > 1:1691881‑M2/1‑62 (MQ=255) gCGTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcacaggcg > 1:1691887‑M2/1‑62 (MQ=255) cGTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcacaggcgg > 1:784511‑M2/1‑61 (MQ=255) tCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcg > 2:363640‑M2/1‑59 (MQ=255) tCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcg > 1:458231‑M2/1‑59 (MQ=255) cTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcga > 2:14125‑M2/1‑58 (MQ=255) ggACGCTAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagc < 1:757804‑M2/76‑21 (MQ=255) ccAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagcacgtt > 1:1151260‑M2/1‑51 (MQ=255) cAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagcacgtta < 1:423037‑M2/76‑27 (MQ=255) aGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagcacgttag < 1:256439‑M2/76‑28 (MQ=255) aGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaat > 2:525347‑M2/1‑44 (MQ=255) gCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaata > 2:1500297‑M2/1‑43 (MQ=255) aaTTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgct < 1:153398‑M2/76‑38 (MQ=255) aaTTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgct > 2:1599941‑M2/1‑39 (MQ=255) aTTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctg < 1:531299‑M2/76‑39 (MQ=255) tGCTCTCTTATCAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgat > 1:899154‑M2/1‑36 (MQ=255) tGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATAc < 2:600522/76‑1 (MQ=255) ctctTATCAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggc > 1:300714‑M2/1‑32 (MQ=255) ctTATCAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggcca < 1:989995‑M2/76‑47 (MQ=255) ttATCAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccag < 2:1175266‑M2/76‑48 (MQ=255) aTCAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaa > 2:1638130‑M2/1‑27 (MQ=255) aTCAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaa > 2:1341186‑M2/1‑27 (MQ=255) tCAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaag > 2:535126‑M2/1‑26 (MQ=255) cAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaaga < 1:1698268‑M2/76‑52 (MQ=255) cAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaaga < 1:777536‑M2/76‑52 (MQ=255) cAAGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaaga < 1:12114‑M2/76‑52 (MQ=255) aaGACAACAACATCGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagat > 2:1038131‑M2/1‑24 (MQ=255) aaGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagat > 2:1747565‑M2/1‑24 (MQ=255) aaGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagat < 2:22658‑M2/76‑53 (MQ=255) aaGACAACAACAACGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagat > 1:358565‑M2/1‑24 (MQ=255) acaacaacaACGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatcca > 2:817183‑M2/1‑21 (MQ=255) acaacaacaACGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatcca > 2:347355‑M2/1‑21 (MQ=255) acaacaacGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccagat > 1:1255854‑M2/1‑18 (MQ=255) caacaacGTGCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccagatg > 1:812630‑M2/1‑17 (MQ=255) aacaacGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccagatgg > 2:864144‑M2/1‑16 (MQ=255) acaacGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccagatgga < 2:1286244‑M2/76‑62 (MQ=255) caacGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccagatggaa > 1:448957‑M2/1‑14 (MQ=255) acGTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccagatggaagc > 1:1151679‑M2/1‑12 (MQ=255) gTTCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccagatggaagcgg > 1:1453680‑M2/1‑10 (MQ=255) tCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccagatggaagcggac < 2:343992‑M2/76‑69 (MQ=255) tCTGGATCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccagatggaagcagac < 2:43068‑M2/76‑69 (MQ=255) aTCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccagatggaagcggacctgcg < 1:1720688‑M2/76‑74 (MQ=255) tCcacaggcacgggcggcgagcacgttagtgaatacgctgatgggccagaagatccagatggaagcggacctgcgg > 1:1357246‑M2/1‑2 (MQ=255) tCcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccagatggaagcggacctgcgg < 2:46233‑M2/76‑75 (MQ=255) | AAGGCACGCCAGCGTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATACGCTGATGGGCCAGAAGATCCAGATGGAAGCGGAC > REL606/3014008‑3014154 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |