| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 17,340 | T→C | intergenic (‑382/‑148) | mokC ← / → nhaA | regulatory protein for HokC, overlaps CDS of hokC/pH‑dependent sodium/proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 17,340 | 0 | T | C | 98.3% | 241.8 / ‑3.8 | 60 | intergenic (‑382/‑148) | mokC/nhaA | regulatory protein for HokC, overlaps CDS of hokC/pH‑dependent sodium/proton antiporter |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (33/26); total (33/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01 | |||||||||||
TTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACA > REL606/17267‑17409 | ttaatCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCa > 1:916880/1‑76 (MQ=255) taatCTCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCaa > 1:192101/1‑76 (MQ=255) aatCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCaaa > 1:1046302/1‑76 (MQ=255) aatCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCaaa > 1:1281944/1‑76 (MQ=255) atCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCaaaa < 2:1009557/76‑1 (MQ=255) ccTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAAc > 1:749593/1‑76 (MQ=255) cTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACt < 1:1233436/76‑1 (MQ=255) cTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACt < 2:1303281/76‑1 (MQ=255) cTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACt > 1:459631/1‑76 (MQ=255) cTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACt < 2:1318582/76‑1 (MQ=255) tCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTc < 1:60290/76‑1 (MQ=255) cAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCa > 2:1153219/1‑76 (MQ=255) aaTCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCAtctc > 1:1055846/1‑76 (MQ=255) ccTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCAcc > 1:831793/1‑76 (MQ=255) ccTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCAcc > 1:13692/1‑76 (MQ=255) ccTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCAcc > 1:1321858/1‑76 (MQ=255) cTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCg < 2:1405924/76‑1 (MQ=255) cTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCg < 1:586547/76‑1 (MQ=255) tCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGc > 1:934429/1‑76 (MQ=255) gccTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTg < 1:1622367/75‑1 (MQ=255) aCCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTg > 1:1517741/1‑76 (MQ=255) ccTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGa > 1:734166/1‑76 (MQ=255) tAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATgg > 2:294160/1‑76 (MQ=255) tAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATgg > 1:1721991/1‑76 (MQ=255) aaGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGgc > 2:1655136/1‑76 (MQ=255) gCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGgcgc < 1:1022575/76‑1 (MQ=255) cTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCa < 1:61822/76‑1 (MQ=255) cTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCa < 1:1092416/76‑1 (MQ=255) cTATGATTATCTGGGCCTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCa > 2:1665882/1‑76 (MQ=255) aTTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAAttc > 2:1304999/1‑76 (MQ=255) aTCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAAttcttc < 2:149642/76‑1 (MQ=255) cTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCaa < 2:1442741/76‑1 (MQ=255) gggCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATg > 1:533352/1‑76 (MQ=255) gggCTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATg < 2:383042/76‑1 (MQ=255) cTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGAt < 2:912362/76‑1 (MQ=255) cTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGAt < 2:876653/76‑1 (MQ=255) cTTAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGAt < 2:61049/76‑1 (MQ=255) tAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCg > 2:112936/1‑76 (MQ=255) tAGGGTCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCg > 2:804608/1‑76 (MQ=255) tCACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTaaaa > 1:1681507/1‑76 (MQ=255) cACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTaaaaa > 2:1766896/1‑76 (MQ=255) cACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTaaaaa < 1:122807/76‑1 (MQ=255) cACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTaaaaa < 1:1559842/76‑1 (MQ=255) cACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTaaaaa > 1:1597037/1‑76 (MQ=255) cACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTaaaaa < 1:30783/76‑1 (MQ=255) cACTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTaaaaa < 1:469769/76‑1 (MQ=255) cTCGTGAGCGCTTACAGCCGCCAGAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAAc > 2:1162947/1‑76 (MQ=255) cTCGTGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAAc > 2:26676/1‑76 (MQ=255) tGAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGt > 2:776716/1‑76 (MQ=255) gAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAgat > 1:621320/1‑74 (MQ=255) gAGCGCTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGtt > 1:1246151/1‑76 (MQ=255) cgcTTACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTAtt < 1:758176/76‑1 (MQ=255) ttACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCt > 1:210393/1‑76 (MQ=255) ttACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCt < 2:189894/76‑1 (MQ=255) ttACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCt > 1:1522394/1‑76 (MQ=255) tACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTa < 2:1083775/76‑1 (MQ=255) tACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTa < 2:797273/76‑1 (MQ=255) tACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTa < 2:424551/76‑1 (MQ=255) tACAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTa < 2:40438/76‑1 (MQ=255) aCAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTac > 1:954141/1‑76 (MQ=255) cAGCCGCCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTaca > 1:294630/1‑76 (MQ=255) | TTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACA > REL606/17267‑17409 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |