| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,202,603 | A→G | intergenic (‑44/‑156) | iclR ← / → metH | DNA‑binding transcriptional repressor/B12‑dependent methionine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,202,603 | 0 | A | G | 98.3% | 244.8 / ‑4.3 | 59 | intergenic (‑44/‑156) | iclR/metH | DNA‑binding transcriptional repressor/B12‑dependent methionine synthase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (30/28); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
GCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGT > REL606/4202530‑4202674 | gCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTCTTAtt < 2:1508095/76‑1 (MQ=255) ccGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTttgttg > 2:1592914/1‑76 (MQ=255) cgTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTa > 1:487921/1‑76 (MQ=255) gTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAg < 1:1187609/76‑1 (MQ=255) tttCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGc < 2:325467/76‑1 (MQ=255) ttCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCt > 2:736466/1‑76 (MQ=255) cgcgGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTaa > 1:590334/1‑76 (MQ=255) gcgGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATCTTCATTTTTGTTGTTAGCTAAt < 2:924997/76‑1 (MQ=255) cgGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATg < 2:748451/76‑1 (MQ=255) cgGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATg < 2:23815/76‑1 (MQ=255) ggAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCa > 1:606066/1‑76 (MQ=255) ggAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCa > 1:1616288/1‑76 (MQ=255) aaTGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAAt > 2:1362470/1‑76 (MQ=255) aTGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATa > 1:1027616/1‑76 (MQ=255) aTGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATa < 2:594336/76‑1 (MQ=255) tGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAg > 2:1426966/1‑76 (MQ=255) gCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCt < 2:683341/76‑1 (MQ=255) gACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGa < 2:1325757/76‑1 (MQ=255) cATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACt > 2:452733/1‑76 (MQ=255) aTGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTg > 1:1738491/1‑76 (MQ=255) gACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGAt > 1:174718/1‑76 (MQ=255) gACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGAt < 1:574158/76‑1 (MQ=255) gACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGAt < 2:355226/76‑1 (MQ=255) aCAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATc > 1:95826/1‑76 (MQ=255) aGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCg > 1:742000/1‑76 (MQ=255) tctcCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGAt > 1:515827/1‑76 (MQ=255) tctcCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGAt > 1:1392891/1‑76 (MQ=255) tctcCTTTTTCTGTATCGTGGAAATCAATTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGAt < 1:1479060/76‑1 (MQ=255) ctcCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATg < 1:1474212/76‑1 (MQ=255) ctcCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATg > 1:666924/1‑76 (MQ=255) ctcCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATg < 1:330446/76‑1 (MQ=255) ctcCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATg < 1:80492/76‑1 (MQ=255) tcCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGa > 1:483414/1‑76 (MQ=255) ccTTTTTCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAg < 1:1002987/76‑1 (MQ=255) tttttCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGtt < 2:1358426/76‑1 (MQ=255) ttttCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTa < 1:1737565/76‑1 (MQ=255) ttCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAAt < 1:1642346/76‑1 (MQ=255) tCTGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATg > 1:1496445/1‑76 (MQ=255) tGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGtt > 1:987363/1‑76 (MQ=255) tGTATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGtt > 2:1486468/1‑76 (MQ=255) tGNATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGtt > 2:1246634/1‑76 (MQ=255) tATCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGa < 1:1275609/76‑1 (MQ=255) aTCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGaa > 1:771810/1‑76 (MQ=255) aTCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGaa > 1:364081/1‑76 (MQ=255) tCGTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGAAc < 2:817843/76‑1 (MQ=255) gTGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGAACaa < 1:126492/76‑1 (MQ=255) tGGAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGAACaaa > 2:1370914/1‑76 (MQ=255) ggAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGAACAAAt > 2:1270996/1‑76 (MQ=255) ggAAATCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGAACAAAt > 1:1093332/1‑76 (MQ=255) aaaTCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGAACAAAtct > 1:779404/1‑76 (MQ=255) aaTCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGAACAAAtctc < 1:1592914/76‑1 (MQ=255) aTCATTTTCATTTTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCa < 1:1696184/76‑1 (MQ=255) tcattttcatttTTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCAt < 1:1222291/76‑1 (MQ=255) attttcattttTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGt < 1:484304/76‑1 (MQ=255) attttcattttTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGt < 1:755714/76‑1 (MQ=255) ttttcattttTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGtt < 2:1616288/76‑1 (MQ=255) ttcattttTGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGc > 1:873153/1‑76 (MQ=255) tttttGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCgtgg > 1:1196517/1‑76 (MQ=255) ttttGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCgtggt < 2:245026/76‑1 (MQ=255) ttttGTTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCgtggt > 1:958653/1‑76 (MQ=255) | GCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGT > REL606/4202530‑4202674 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |