Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,349,335	T→A	intergenic (‑202/‑74)	yjeH ← / → groES	predicted transporter/co‑chaperonin GroES

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,349,335	0	T	A	100.0%	220.3 / NA	59	intergenic (‑202/‑74)	yjeH/groES	predicted transporter/co‑chaperonin GroES
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (36/23);  total (36/23)

ATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTT  >  REL606/4349264‑4349404                                                                        |                                                                      aTTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACAcc                                                                   <  1:1593495/76‑1 (MQ=255)  ttttGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCa                                                                  <  1:371518/76‑1 (MQ=255)   tttGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAg                                                                 <  2:1334651/76‑1 (MQ=255)       tgCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCgg                                                             >  1:104339/1‑76 (MQ=255)          tGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGaa                                                          >  2:796746/1‑76 (MQ=255)          tGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGaa                                                          <  1:713660/76‑1 (MQ=255)          tGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGaa                                                          >  1:1690382/1‑76 (MQ=255)           gATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGaaa                                                         >  2:1102587/1‑76 (MQ=255)                    tttttttCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAg                                                <  2:1061510/76‑1 (MQ=255)                       ttttctttttcCCCCTTTAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTc                                             >  1:8702/1‑76 (MQ=255)                       ttttctttttcCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTc                                             <  2:996481/76‑1 (MQ=255)                       ttttctttttcCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTc                                             <  1:630523/76‑1 (MQ=255)                       ttttctttttcCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTc                                             >  1:1510893/1‑76 (MQ=255)                        tttctttttcCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTcc                                            >  1:1230289/1‑76 (MQ=255)                           ctttttcCCCCTTGAACGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGc                                         >  2:1284106/1‑76 (MQ=255)                            tttttcCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCg                                        >  1:680115/1‑76 (MQ=255)                             ttttcCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGgtgt                                       <  1:1676402/76‑1 (MQ=255)                             ttttcCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGt                                       >  1:1551454/1‑76 (MQ=255)                              tttcCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTc                                      <  2:1395990/76‑1 (MQ=255)                              tttcCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTc                                      >  1:1233001/1‑76 (MQ=255)                                tcCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCAc                                    >  2:157992/1‑76 (MQ=255)                                tcCCCCTGGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCAc                                    <  2:10760/76‑1 (MQ=255)                                 cccccTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCAcc                                   >  1:279292/1‑76 (MQ=255)                                 cccccTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCAcc                                   <  1:1368211/76‑1 (MQ=255)                                 cccccTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCAcc                                   >  2:237123/1‑76 (MQ=255)                                  ccccTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCAccc                                  >  2:1654506/1‑76 (MQ=255)                                   cccTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCa                                 >  1:746780/1‑76 (MQ=255)                                   cccTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCa                                 <  2:998157/76‑1 (MQ=255)                                    ccTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCAt                                >  2:271662/1‑76 (MQ=255)                                    ccTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCAt                                >  1:419533/1‑76 (MQ=255)                                    ccTTAAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCAt                                <  2:571268/76‑1 (MQ=255)                                     cTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATa                               >  2:875486/1‑76 (MQ=255)                                     cTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATa                               >  1:968581/1‑76 (MQ=255)                                      ttGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATaa                              >  1:1354250/1‑76 (MQ=255)                                      ttGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATaa                              <  1:408544/76‑1 (MQ=255)                                       tGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAAc                             >  1:970697/1‑76 (MQ=255)                                        gcnggggCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACa                            >  2:1246594/4‑76 (MQ=255)                                        gAAGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACa                            >  2:282285/1‑76 (MQ=255)                                         aaGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAg                           <  1:852165/76‑1 (MQ=255)                                         aaGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAg                           <  1:791423/76‑1 (MQ=255)                                         aaGGGGCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAg                           >  2:723706/1‑76 (MQ=255)                                         aaGGGGCCAAGCCTCATCCCCATTGCTCGGGACGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAg                           <  1:490674/76‑1 (MQ=255)                                            gggCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATa                        >  1:1192874/1‑76 (MQ=255)                                              gCGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACg                      <  2:675393/76‑1 (MQ=255)                                               cGAAGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACgg                     <  2:1712429/76‑1 (MQ=255)                                                 aaGCCTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGAc                   >  2:1107357/1‑76 (MQ=255)                                                    ccTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACttt                <  1:1069806/76‑1 (MQ=255)                                                    ccTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACttt                <  2:104339/76‑1 (MQ=255)                                                     cTCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTtc               >  1:402304/1‑76 (MQ=255)                                                      tCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTtct              >  1:1524089/1‑76 (MQ=255)                                                      tCATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTtct              >  1:1524118/1‑76 (MQ=255)                                                       cATCCCCATTTCTCTGGACTCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTtctc             >  2:1737741/1‑76 (MQ=255)                                                       cATCCCCATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTtctc             >  2:1177567/1‑76 (MQ=255)                                                           cccATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAg         >  1:253857/1‑76 (MQ=255)                                                           cccATTTCTCGGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAg         <  1:687577/76‑1 (MQ=255)                                                            ccATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAgg        >  1:1253325/1‑76 (MQ=255)                                                            ccATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAgg        <  1:936123/76‑1 (MQ=255)                                                             cATTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGga       <  2:309511/76‑1 (MQ=255)                                                              aTTTCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGgag      <  2:185198/76‑1 (MQ=255)                                                               tttCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGgaga     >  1:1457469/1‑76 (MQ=255)                                                                ttCTCTGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGagag    >  1:100646/1‑76 (MQ=255)                                                                ttCTCTCGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGagag    <  1:613217/76‑1 (MQ=255)                                                                  ctctGGACACCAGCTGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGtt  <  1:104860/76‑1 (MQ=255)                                                                  ctctGGACACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGtt  <  2:1009304/76‑1 (MQ=255)                                                                        |                                                                      ATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTT  >  REL606/4349264‑4349404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑