Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,609,130	A→C	K285Q (AAG→CAG)	insF‑3 →	putative transposase for IS3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,609,130	0	A	C	100.0%	78.5 / NA	20	K285Q (AAG→CAG)	insF‑3	putative transposase for IS3
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (7/13);  total (7/13)

GGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGCATTGCTGT  >  REL606/1609103‑1609201                            |                                                                        ggcCTCAGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTc                         >  2:1243693/1‑76 (MQ=14)     tCAGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCt                     >  2:358623/1‑76 (MQ=255)     tCAGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCt                     <  2:1166178/76‑1 (MQ=255)      cAGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTa                    <  1:417585/76‑1 (MQ=255)       aGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTaa                   <  2:1400654/76‑1 (MQ=255)        gTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTaaa                  <  1:326635/76‑1 (MQ=255)         tCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAAt                 >  2:552048/1‑76 (MQ=255)         tCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAAt                 <  2:465118/76‑1 (MQ=255)          ccGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATa                <  1:1382921/76‑1 (MQ=255)            ggAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACg              <  1:1562354/76‑1 (MQ=255)             gAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGa             <  1:235811/76‑1 (MQ=255)                cAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGc          <  2:1746694/76‑1 (MQ=255)                cAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGc          <  2:760515/76‑1 (MQ=255)                     tGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGCATTGc     >  2:1318888/1‑76 (MQ=255)                     tGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGCATTGc     >  2:1464407/1‑76 (MQ=255)                     tGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGCATTGc     >  2:757470/1‑76 (MQ=255)                      gAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGCATTGCt    <  1:171670/76‑1 (MQ=255)                       aaaaCCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGCATTGCTg   >  2:1004684/1‑76 (MQ=255)                       aaaaCCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGCATTGCTg   <  2:164748/76‑1 (MQ=255)                        aaaCCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGCATTGCTGt  <  2:1092125/76‑1 (MQ=255)                        aaaCCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGCATTGCTGt  <  1:768558/76‑1 (MQ=255)                            |                                                                        GGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGCATTGCTGT  >  REL606/1609103‑1609201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑