| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,886,192 | A→C | intergenic (‑22/+647) | yobF ← / ← yebO | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,886,192 | 0 | A | C | 95.5% | 284.9 / ‑0.1 | 67 | intergenic (‑22/+647) | yobF/yebO | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (3/0); new base C (28/36); total (31/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.38e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
ATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCAC > REL606/1886119‑1886264 | aTTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCAc < 2:871911/76‑1 (MQ=255) ttCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCAcc > 2:1048711/1‑76 (MQ=255) caacgtcaacgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCg > 2:26590/1‑76 (MQ=255) aacgtcaacgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGa > 1:1416287/1‑76 (MQ=255) cgtcaacgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGaaa < 1:302584/76‑1 (MQ=255) cgtcaacgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGaaa < 2:260291/76‑1 (MQ=255) gtcaacgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAAt < 1:800996/76‑1 (MQ=255) tcaacgtGTTTACTCATGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATc < 1:1049764/76‑1 (MQ=255) tcaacgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATc > 2:1565689/1‑76 (MQ=255) caacgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCt < 1:1319307/76‑1 (MQ=255) aacgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTg > 2:856915/1‑76 (MQ=255) cgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGtt < 1:1646304/76‑1 (MQ=255) tgtTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTAt < 2:1063539/76‑1 (MQ=255) gtTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATc < 2:1009652/76‑1 (MQ=255) tttACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATca < 2:541708/76‑1 (MQ=255) tttACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATca > 2:721937/1‑76 (MQ=255) ttACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATcac > 2:1459896/1‑76 (MQ=255) tACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATcaca > 1:1078042/1‑76 (MQ=255) cTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATa > 1:62971/1‑76 (MQ=255) cTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATa < 1:1282272/76‑1 (MQ=255) cAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACa < 2:429015/76‑1 (MQ=255) cAGGACTTCTTTACTGAAAATGCAACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACa > 2:1690412/1‑76 (MQ=255) aGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACaa < 1:1455274/76‑1 (MQ=255) aGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACaa < 2:463623/76‑1 (MQ=255) ggACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAAc < 2:69815/76‑1 (MQ=255) ggACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAAc > 2:426387/1‑76 (MQ=255) ggACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAAc < 1:827291/76‑1 (MQ=255) ggACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAAc < 1:933669/76‑1 (MQ=255) gACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACg > 1:1502890/1‑76 (MQ=255) ttcttTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTa < 2:1196818/76‑1 (MQ=255) cttTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAAt > 1:1010220/1‑76 (MQ=255) tttACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAAtt > 1:760948/1‑76 (MQ=255) ttACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTa > 2:854030/1‑76 (MQ=255) tACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTAt < 2:671649/76‑1 (MQ=255) aCTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATg > 1:1118923/1‑76 (MQ=255) tGAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGc > 1:877703/1‑76 (MQ=255) gAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCa < 2:1705716/76‑1 (MQ=255) gAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCa < 2:1083074/76‑1 (MQ=255) gAAAATGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCa < 1:1442074/76‑1 (MQ=255) aaaaTGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCaa > 1:1533315/1‑76 (MQ=255) aaaTGCCACACATAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAg > 2:1001289/1‑76 (MQ=255) cacacaTAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCAttt > 2:526/1‑76 (MQ=255) cacacaTAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCAttt < 2:1668366/76‑1 (MQ=255) cacaTAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCAttttt > 2:781775/1‑76 (MQ=255) caTAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTaa < 2:137294/76‑1 (MQ=255) caTAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTaa < 2:181314/76‑1 (MQ=255) tAAACAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAAc < 1:1243294/76‑1 (MQ=255) aaaCAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACa > 2:935103/1‑76 (MQ=255) aaaCAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACa > 1:1645097/1‑76 (MQ=255) aCAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATg < 1:119321/76‑1 (MQ=255) cAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGt < 2:229135/76‑1 (MQ=255) cAGAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGt > 1:250948/1‑76 (MQ=255) gAACTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGtct > 1:95780/1‑76 (MQ=255) aaCTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGtctc < 1:818418/76‑1 (MQ=255) aCTGTACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCg < 1:843474/76‑1 (MQ=255) tACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGTCATTTTTAAACATGTCTCGATCa > 2:1402611/1‑76 (MQ=255) tACCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCa > 2:1014938/1‑76 (MQ=255) aCCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAg < 1:370429/76‑1 (MQ=255) aCCTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAg < 1:1114744/76‑1 (MQ=255) ccTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGa < 1:605972/76‑1 (MQ=255) ccTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGa < 2:1068184/76‑1 (MQ=255) cTCGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGAc > 1:1511796/1‑76 (MQ=255) cGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGc > 1:1112264/1‑76 (MQ=255) cGTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGc > 1:1040118/1‑76 (MQ=255) gTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGca < 1:325408/76‑1 (MQ=255) gTTTCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGca > 2:88261/1‑76 (MQ=255) tttCACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGcac < 2:1722678/76‑1 (MQ=255) | ATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCAC > REL606/1886119‑1886264 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |