| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,874,154 | (GT)5→4 | coding (513‑514/936 nt) | lysR → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,874,146 | 0 | G | . | 94.1% | 142.3 / 4.6 | 34 | coding (505/936 nt) | lysR | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base . (23/9); total (23/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.80e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
| * | REL606 | 2,874,147 | 0 | T | . | 96.9% | 136.0 / 2.3 | 34 | coding (506/936 nt) | lysR | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (0/1); major base . (22/9); minor base A (0/1); total (23/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
CATGATTTAGGACTCACTGAAACGCTCCATACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAGTGTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCGGATGATTTTCAGGGTG > REL606/2874071‑2874215 || cATGANTCAGGACTCACTGAAACGCTCCTTACGCCTGCGGGAACAGAACGTACCGAATTACNCTCTTTAGATGAAg > 2:1718828/1‑76 (MQ=255) gATTTAGGACTCACTGAAACGCTCCATACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAgtgt > 1:875340/1‑76 (MQ=255) tttAGGACTCACTGAAACGCTCCATACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAgtgtgt > 1:1212034/1‑76 (MQ=255) aGGACTCACTGAAACGCTCCATACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAgtgtgtgtt < 1:1519190/76‑2 (MQ=255) ggACTCACTGAAACGCTCCATACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAgtgtgtgtta < 2:1611315/76‑3 (MQ=255) ggACTCACTGAAACGCTCCATACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAgtgtgtgtta < 2:1420575/76‑3 (MQ=255) gAAACGCTCCATACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCCGGGt > 2:543304/1‑76 (MQ=255) gAAACGCTCCATACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCCGGGt > 2:396908/1‑76 (MQ=255) aaaCGCTCCATACGCCTGCGGGAACAGAACGTACCGAATTGCTCTCTTTAGATGA‑‑CGTGTGTGTTACCTCCGGGTc > 2:1363265/1‑76 (MQ=255) cGCTCCATACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATc > 2:164333/1‑76 (MQ=255) gCTCCATACGCCTGCGGGAANAGAACGTACAGAATTNCTCTCTTTAGATG‑‑GACTGTGTCTTACCTCCGGGTCATcc < 2:1698907/76‑1 (MQ=255) gCTCCATACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATcc > 1:502793/1‑76 (MQ=255) gCTCCATACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATcc > 1:502796/1‑76 (MQ=255) cATACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTg > 2:615336/1‑76 (MQ=255) aTACGNCGGAGGGAACAGAACGTACCGACATACTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCNGGGTCATCCGCTgg > 2:1711019/1‑76 (MQ=255) aTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTgg > 1:476932/1‑76 (MQ=255) aTACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTgg > 2:1231784/1‑76 (MQ=255) ccTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTa < 1:1295386/76‑1 (MQ=255) aaCAGAACGTACCGNATTACTCTCGTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGt > 1:8268/1‑76 (MQ=255) aaCAGAACGTACCGAATTACTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGt > 1:118230/1‑76 (MQ=255) aaCAGAACGTACCGAATTACTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGt < 2:1293642/76‑1 (MQ=255) cAGAACGTACCGAATTACTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTAt > 1:1524756/1‑76 (MQ=255) aaCGTACCGAATTACTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTaa < 1:796892/76‑1 (MQ=255) gTACCGAATTACTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGc > 2:124127/1‑76 (MQ=255) tACCGAATTACTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGcc > 1:75456/1‑76 (MQ=255) aCCGAATTACTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCg > 2:1029384/1‑76 (MQ=255) aCCGAATTACTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCg > 1:938966/1‑76 (MQ=255) aCCGAATTACTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCg > 1:813241/1‑76 (MQ=255) ccGAATTACTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCgg < 1:277771/76‑1 (MQ=255) cGAATTACTCTCTTTAGATGAAGTGTG‑G‑GTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCGga < 1:173649/76‑1 (MQ=255) aaTTACTCTCTTTANATGAA‑‑GTGTGTGTTACCTCCGGGTCATACGCTGGCGGTAAAAAAGTTAGTAACGCCGgatg < 1:1466549/76‑1 (MQ=255) tACTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCGGATGAtt > 2:721882/1‑76 (MQ=255) aCTCTCTTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGAATTAACGCCGGATGAttt > 2:52671/1‑76 (MQ=255) tctctTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCGGATGATTTTc < 2:1145979/76‑1 (MQ=255) ctTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCGGATGATTTTCAgg < 1:1095913/76‑1 (MQ=255) ctTTAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCGGATGATTTTCAgg < 1:1518208/76‑1 (MQ=255) tttAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCGGATGATTTTCAggg > 2:1012426/1‑76 (MQ=255) tttAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCGGATGATTTTCAggg > 2:641908/1‑76 (MQ=255) ttAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCGGATGATTTTCAGGGt < 1:156901/76‑1 (MQ=255) tAGATGAA‑‑GTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCGGATGATTTTCAGGGTg > 2:735510/1‑76 (MQ=255) || CATGATTTAGGACTCACTGAAACGCTCCATACGCCTGCGGGAACAGAACGTACCGAATTACTCTCTTTAGATGAAGTGTGTGTGTTACCTCCGGGTCATCCGCTGGCGGTAAAAAAGGTATTAACGCCGGATGATTTTCAGGGTG > REL606/2874071‑2874215 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |