Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	120.1 / NA	40	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/18);  total (22/18)

GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC  >  REL606/2193706‑2193850                                                                          |                                                                        gCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcc                                                                       <  1:817305/76‑1 (MQ=255)   cGATGCATCCGACGTCCAGGCGCGGCTGGTCTGGCTGNCGGAAATCAGCCCCAATAACAGCAAAAAGCCAATcccc                                                                     >  1:1669201/1‑76 (MQ=255)   cGANGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcccc                                                                     >  1:1546647/1‑76 (MQ=255)    gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                                                                    <  1:1125879/76‑1 (MQ=255)    gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                                                                    >  1:852591/1‑76 (MQ=255)    gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGAAATCAGCCCCAATAACAACAAAACGCCAACCCcca                                                                    >  1:375608/1‑76 (MQ=255)      tGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacc                                                                  <  1:121566/76‑1 (MQ=255)       gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCACTAACAGCAAAACGCCAATCCccacca                                                                 >  1:939662/1‑76 (MQ=255)       gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGGAAAACGCCAATCCccacca                                                                 >  1:1251761/1‑76 (MQ=255)       gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacca                                                                 >  1:1442827/1‑76 (MQ=255)       gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacca                                                                 >  1:1558303/1‑76 (MQ=255)       gCATCCGGCGACCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacca                                                                 >  1:1613225/1‑76 (MQ=255)         aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGa                                                               <  2:858075/76‑1 (MQ=255)          tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCCGAt                                                              >  2:852765/1‑76 (MQ=255)          tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAAAAGGAAAACGCCAATCCCCACCAGAt                                                              >  2:313592/1‑76 (MQ=255)              gCGTCCAGGCGCGGATGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCTATACCCTCCAGATAAAg                                                          <  1:134587/76‑1 (MQ=255)               cGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCGGCCACAATAACAGCAAAACGCCAATCCCCGCCAGATAAAGc                                                         <  1:48376/76‑1 (MQ=255)                gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa                                                        >  2:1293980/1‑76 (MQ=255)                gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa                                                        >  1:592888/1‑76 (MQ=255)                  cgaGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                                                      <  1:556148/74‑1 (MQ=255)                  ccAGGCGCGGCTGGTCCGGCTGTCGGAAATCAGCCCCAAGAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                                                      <  1:40678/76‑1 (MQ=255)                  ccAGGCGCGGCTGGGCTGGCTGTCGGAAATCAGCGCCAATANCCGCAAAACGCCAATCCCNACNCGATAAAGCATg                                                      >  1:1404766/1‑76 (MQ=255)                   cAGNCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa                                                     >  1:1544064/1‑76 (MQ=255)                    aGGCGCGGCTGGTCTGGCTGTCGGGAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc                                                    >  2:455525/1‑76 (MQ=255)                    aGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc                                                    <  1:1292740/76‑1 (MQ=255)                             tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                           <  2:427353/76‑1 (MQ=255)                             tGGTCGGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCACGACGCCTGTAtt                                           <  2:59107/76‑1 (MQ=255)                              ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                          <  2:480978/76‑1 (MQ=255)                               gTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                         >  1:1377058/1‑76 (MQ=255)                                 cTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                       >  1:546009/1‑76 (MQ=255)                                  tGGCTGTCCGAAATCAGCCCCGATAACAGCAAAACGCCAATCCCCCGCAGATAAAGCATGATGCCTGTATTAAACa                                      >  2:24259/1‑76 (MQ=255)                                   ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                     >  2:324072/1‑76 (MQ=255)                                        tCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                >  2:422339/1‑76 (MQ=255)                                         cGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                               >  1:1553907/1‑76 (MQ=255)                                          ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                              <  2:1477818/76‑1 (MQ=255)                                            aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                            >  1:755183/1‑76 (MQ=255)                                            aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                            >  1:585269/1‑76 (MQ=255)                                             aaTCAGCCCCAATAACAGCAAAACGCCAATCCCCGCCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc                           <  1:561490/76‑1 (MQ=255)                                             aaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc                           <  1:1107820/76‑1 (MQ=255)                                              aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGTTAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                          <  1:422340/76‑1 (MQ=255)                                                   ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                     <  1:815941/76‑1 (MQ=255)                                                         tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg               >  2:847687/1‑76 (MQ=255)                                                               cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGTGCAGCGCGCAcc         >  2:292808/1‑76 (MQ=255)                                                                 aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg       <  1:1453944/76‑1 (MQ=255)                                                                  aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc      >  2:737883/1‑76 (MQ=255)                                                                      ccNATCCCCACCAGATAAAGCATGACGCCTGTATTAAACGGGCTTTTTACCGATGGCGCATCGCGCAGCAgctncc  <  2:1400400/76‑1 (MQ=255)                                                                      ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc  >  1:325932/1‑76 (MQ=255)                                                                          |                                                                        GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC  >  REL606/2193706‑2193850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑