Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,348,313	C→A	L117L (CTG→CTT)	nuoA ←	NADH dehydrogenase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,348,313	0	C	A	100.0%	112.3 / NA	30	L117L (CTG→CTT)	nuoA	NADH dehydrogenase subunit A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/6);  total (24/6)

TGTTCGTTTCCGGGTTCATACGCTCGCGGCGTGAACGCGCGGGCGTCCAGTCCAGCGCGCCAATACGCACCAGATAAACCAGACCTGCCAGTAACACAAAAATAAAAATTGCAGCTTCCACAAAGCCTACCCAGCCGCT  >  REL606/2348243‑2348381                                                                       |                                                                     tGTTCGTTTCCGGGTTCATACGCTCGCGGCGTGAACGCGCGGGCGTCCAGTCCAGCGCGCCAATACGCACAAGATa                                                                 <  1:1416062/76‑1 (MQ=255) tGTTAGTCTCCGGGTTCATACGCTCGCGGCGTGAACGGGCGGGCGTCCAGTCCAGCGCGCCAATACGCACAAGATa                                                                 <  2:1678661/76‑1 (MQ=255)   ttCGTTTCCGGGTTCATACGCTCGCGGCGTGAACGCACGGTCGTCCAGTCCAGCGCGCCAATACGCACAAGATaaa                                                               >  2:1361200/1‑76 (MQ=255)    tCGTTTCCGGGTTCATCCGCTCGCGGCGTGAACGCGCGGGCGTCCAGTCCAGCGCGCCAATACGCACAAGATAAAc                                                              <  1:987334/76‑1 (MQ=255)     cGTTTCCGGGTTCATACGCTCGCGGCGTGAACGCGCGGGCGTCCAGTCCAGCGCGCCAATACGCACAAGATAAAcc                                                             <  2:757859/76‑1 (MQ=255)      gTTTCCGGGTTCATACGCTCGCGGCGTGAACGCGCGGGCGTCCAGTCCAGCGCGCCAATACGCACAAGATAAACCa                                                            >  1:888430/1‑76 (MQ=255)        ttCCGGGTTCATACGCTCGCGGCGTGAACGCGCGGGCGTCCAGTCCAGCGCGCCAATACGCACAAGATAAACCAGa                                                          <  1:933136/76‑1 (MQ=255)         tCCGGGTTCATACGCTCGCGGCGTGAACGCGCGGGCGTCCAGGCCCGCGCGCCAACACGCACAAGAAAAACCAGAc                                                         >  1:307976/1‑76 (MQ=255)          ccGGGTTCATACGCTCGCGGCGTGAACGCGCGGGCGTCCAGTCCAGCGCGCCAATACGCACAAGATAAACCAGAcc                                                        >  2:1256447/1‑76 (MQ=255)            gggTTCATACGCTCGCGGCGTGAACGCGCGGGCGTCCAGTCCAGCGCGCCAATACGCACAAGATAAACCAGACCTg                                                      >  2:1479052/1‑76 (MQ=255)              gTTCATCCGCCCGCGGCGTGAACGCGCGGGCGTCCAGTCCAGCGCGCCAATACGCACAAGATAAACCAGACCTGcc                                                    <  2:1528575/76‑1 (MQ=255)              gTTCATACGCTCGCGGCGTGAACGCGCGGGCGTCCAGTCCAGCGCGCCAATACGCACAAGATAAACCAGACCTGcc                                                    >  2:776569/1‑76 (MQ=255)                 cATACGCTCGCGGCGTGAACGCGCGGGCGTCCAGTCCAGCGCGCCAATACGCACAAGATAAACCAGACCTGCCAGt                                                 >  2:695174/1‑76 (MQ=255)                         cgcgGCGTGAACGCGCGGGCGTCCAGTCCAGCGCGCCAATACGCACAAGATAAACCAGACCTGACAGTAACACaaa                                         >  2:1567183/1‑76 (MQ=255)                         cgcgGCGCGACCGCGCGGGCGCCCACCCCAGCGCGCAAATACGCAAAAAAAAACCCAGCCCTGCCAGTAACACaaa                                         <  2:804690/76‑1 (MQ=255)                           cggcgTGAACGCGCGGGCGTCCAGTCCAGCGCGCCAATACGCACAAGATAAACCAGACCTGCCAGTAACACaaaaa                                       >  1:429088/1‑76 (MQ=255)                              cgTGAACGCGCGGGCGTCCATTCCAGCGCGCCAATACGCACAAGATAAACCAGACCTGCCAGTAACACaaaaataa                                    >  2:137723/1‑76 (MQ=255)                              cgTGAACGCGCGGGCGTCCAGTCCAGCGCGCCAATACGCACAAGATAAACCAGACCTGCCAGTAACACaaaaataa                                    >  1:1409886/1‑76 (MQ=255)                                 gAACGCGCGGGCGTCCAGTCCAGCGCGCCAATACGCACAAGATAAACCAGACCTGCCAGTAACACaaaaataaaaa                                 >  2:459950/1‑76 (MQ=255)                                  agcgcgcgGGCGTCCAGCCCAGCGCGCCAATACGCAAAAGAAAACCCAGCCCTGCCAGTAACACaaaaataaaaat                                <  1:1228482/74‑1 (MQ=255)                                        ggggCGCCCACACCAGCGCGCAAATCCACATAAAAAAANCCAGACCTGCCAGTAACACAAAAATAAAAATTGCAGc                          <  1:1675036/75‑1 (MQ=255)                                        cgGGCGTCCAGTCCAGCGCGCCAATACGCACAAGATAAACCAGACCTGCCAGTAACACAAAAATAAAAATTGCAGc                          >  1:807128/1‑76 (MQ=255)                                              cccagtccagCGCCCCAATACCCAAAAAAAAACCCAGACCTGCCAGTAACACAAAAATAAAAATTGCAGCTTCcac                    <  1:1445745/75‑1 (MQ=255)                                              cccagcccagcCCCCAAATCCGCAAAAAAAAAACCAGCCCTGCCAGTAACACAAAAATAAAAATTGCAGCTTCcac                    <  1:1601265/75‑1 (MQ=255)                                               ccagtccagCGCGCCAATACGCACAAGATAAACCAGACCTGCCAGTAACACAAAATTAAAAATTGCAGCTTCcaca                   >  1:1694491/1‑76 (MQ=255)                                                   tccagCGCGCCAATACGCACAAGATAAACCAGACCTGCCAGTAACACAAAAATAAAAATTGCAGCTTCCACAAAGc               >  2:1114521/1‑76 (MQ=255)                                                    ccagCGCGCCAATACGCACAAGATAAACCAGACCTGCCAGTAACACAAAAATAAAAATTGCAGCTTCCACAAAGcc              >  1:522555/1‑76 (MQ=255)                                                    ccagCGCGCCAATACGCACAAGATAAACCAGACCTGCCAGTAACACAAAAATAAAAATTGCAGCTTCCACAAAGcc              >  1:410309/1‑76 (MQ=255)                                                    ccagCGCGCCAATACGCACAAGATAAACCAGACCTGCCAGTAACACAAAAATAAAAATTGCAGCTTCCACAAAGcc              >  1:1634859/1‑76 (MQ=255)                                                    ccagCGCGCCAATACGCACAAGATAAACCAGACCTGCCAGTAACACAAAAATAAAAATTGCAGCTTCCACAAAGcc              >  1:1325385/1‑76 (MQ=255)                                                     cagCGCGCCAATACGCACAAGATAAACCAGACCTGCCAGTAACACAAAAATAAAAATTGCAGCTTCCACAAAGCCt             >  1:1440767/1‑76 (MQ=255)                                                      agCGCGCCAATACGCACAAGATAAACCAGACCTGCCAGTAACACAAAAATAAAAATTGCAGCTTCCACAAAGCCTa            >  1:647128/1‑76 (MQ=255)                                                         gcgcCAACACGCAAAAAAAAACCCAGCCCTGCCAGTAACACAAAAATAAAAATTGCAGCTTCCACAAAGCCTAccc         <  2:1314411/76‑1 (MQ=255)                                                           gcCAATACGCACAAGATAAACCAGACCTGCCAGTAACACAAAAATAAAAATTGCAGCTTCCACAAAGCCTACCCAg       >  1:1339896/1‑76 (MQ=255)                                                              aaTACGCACAAGATAAACCAGACCTGCCAGTAACACAAAAATAAAAATTGCAGCTTCCACAAAGCCTACCCAGCCg    >  2:1217061/1‑76 (MQ=255)                                                                tACGCACAAGATAAACCAGACCTGCCAGTAACACAAAAATAAAAATTGCAGCTTCCACAAAGCCTACCCAGCCGCt  >  1:752035/1‑76 (MQ=255)                                                                tACGCACAAGATAAACCAGACCTGCCAGTAACACAAAAATAAAAATTGCAGCTTCCACAAAGCCTACCCAGCCGCt  >  1:1559465/1‑76 (MQ=255)                                                                       |                                                                     TGTTCGTTTCCGGGTTCATACGCTCGCGGCGTGAACGCGCGGGCGTCCAGTCCAGCGCGCCAATACGCACCAGATAAACCAGACCTGCCAGTAACACAAAAATAAAAATTGCAGCTTCCACAAAGCCTACCCAGCCGCT  >  REL606/2348243‑2348381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑