| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,202,378 | T→C | H61R (CAC→CGC) | iclR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,202,378 | 0 | T | C | 97.7% | 160.8 / NA | 43 | H61R (CAC→CGC) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (21/21); minor base A (1/0); total (22/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA > REL606/4202308‑4202450 | gATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGCGGGTc > 1:1310028/1‑76 (MQ=255)gATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGCGGGTc < 2:1295213/76‑1 (MQ=255) tGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGCGGGTCGTg < 2:226154/76‑1 (MQ=255) tGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGCGGGTCGTg > 1:1622955/1‑76 (MQ=255) aTGTCCCANTTCGCCGACCTGACGCACTAAACCGCGCTGTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTg < 2:281597/76‑1 (MQ=255) tGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTgg > 2:1551260/1‑76 (MQ=255) tCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGt < 2:639841/76‑1 (MQ=255) cccAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTa < 2:49202/76‑1 (MQ=255) cAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAAc > 1:1357880/1‑76 (MQ=255) cAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAANTGGGTAAc < 1:1639505/76‑1 (MQ=255) aGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAAcc > 2:215187/1‑76 (MQ=255) ttCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCg > 1:493361/1‑76 (MQ=255) tCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCgg > 2:1491511/1‑76 (MQ=255) cTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTgc > 1:1596549/1‑76 (MQ=255) cTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTgc < 2:1246093/76‑1 (MQ=255) cTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTgc > 2:1272033/1‑76 (MQ=255) tGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTgcg < 2:236300/76‑1 (MQ=255) aCGAAACCCTGCTGTTGCCTTNTGNTTAGCAGACGGCGGGTCGTGGNATTGGTTNANCCGGCTTGTTGCGCCAGtt > 2:242988/1‑76 (MQ=255) aCGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGtt > 2:760806/1‑76 (MQ=255) cGAACCCCTGCGGTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTc < 2:1609145/76‑1 (MQ=255) aaaCCCTGCTGTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCg > 2:581847/1‑76 (MQ=255) aaaCCCTGCTGTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCg > 2:823290/1‑76 (MQ=255) aaaCCCTGCTGTTGCATTGTGGGTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCAGGCTTGTTGCGCCAGTTCCg < 2:143662/76‑1 (MQ=255) ccTGCTGTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAg < 2:1527695/76‑1 (MQ=255) gctgTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGc < 1:482276/76‑1 (MQ=255) ctgTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGcc > 2:514990/1‑76 (MQ=255) ctgTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGcc > 2:819510/1‑76 (MQ=255) ctgTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGcc > 2:881088/1‑76 (MQ=255) tgTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCca < 1:1348388/76‑1 (MQ=255) tgTTGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCca < 1:1318122/76‑1 (MQ=255) ttGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCcaca < 2:871218/76‑1 (MQ=255) ttGCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCcaca < 2:352137/76‑1 (MQ=255) gCATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACt < 1:1033212/76‑1 (MQ=255) cATTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTg > 1:653375/1‑76 (MQ=255) aTTGTGGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGc > 1:1147081/1‑76 (MQ=255) gtgGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCAt < 2:1536617/76‑1 (MQ=255) tgGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCAtt > 2:184019/1‑76 (MQ=255) tgGTTAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCAtt < 2:877827/76‑1 (MQ=255) tttgCAAGCGGAGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGa > 1:1572320/4‑76 (MQ=255) ttAGCAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGa > 2:656392/1‑76 (MQ=255) cAGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCg < 2:361021/76‑1 (MQ=255) aGGCGGCGGGTCGTGGAATTGGTTCACCCGGCTTGTTGCGCCAGTTCCGTGAGAGCGATACTGCCATTGGATTCgg > 1:85314/1‑76 (MQ=255) aGGCGGCGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCgg < 1:227944/76‑1 (MQ=255) cggcggGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCaa > 1:769599/1‑76 (MQ=255) | GATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA > REL606/4202308‑4202450 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |