| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 854,225 | C→A | A312S (GCC→TCC) | ybiW ← | predicted pyruvate formate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 854,225 | 0 | C | A | 97.5% | 122.9 / ‑2.2 | 40 | A312S (GCC→TCC) | ybiW | predicted pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (22/17); total (23/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.15e-01 | |||||||||||
TTGAGTGTGAGCCGGAGCGGATCTTGTTCACTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATAC > REL606/854153‑854296 | ttGAGTGTGAGCCGGAGCGGATCTTGTTCACTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGAgtg < 2:596079/76‑1 (MQ=255) gAGTGTGAGCCGGAGCGGATCTTGTTCACTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGAGTGtt > 1:956328/1‑76 (MQ=255) tgtgAGCCGGAGCGGATCTTGTTCACTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGAGTGTTctc < 2:844403/76‑3 (MQ=255) aGCCGGAGGAGATCTAGTACACTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGCTGGAGTGTTCGCGATc < 1:17731/76‑1 (MQ=255) aGCCGGAGCGGATCTTGTTCACTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGAGTGTTCGCGATc > 1:1351998/1‑76 (MQ=255) gCCGGAGCGGATCTTGTTCACTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGCGTGTTCGCGATcc > 1:1120763/1‑76 (MQ=255) cGGAGCGGATCTTGTTCACTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGAGTGTTCGCGATCCAg > 1:912029/1‑76 (MQ=255) ggAGCGGATCTTGTTCACTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGAGTGTTCGCGATCCAGc > 1:1004962/1‑76 (MQ=255) cGGATCTTGTTCACTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGAGTGTTCGCGATCCAGCGTCt > 2:567855/1‑76 (MQ=255) ggATCTTGTTCACTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGAGTGTTCGCGATCCAGCGTCTg < 2:546146/76‑1 (MQ=255) ggATCTTGTTCACTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGAGTGTTCGCGATCCAGCGTCTg < 1:1225556/76‑1 (MQ=255) gATCTTGTTCACTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGAGTGTTCGCGATCCAGCGTCTgg > 2:1481695/1‑76 (MQ=255) tCTTGTTCACTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGAGTGTTCGCGATCCAGCGTCTGGtt < 1:1159024/76‑1 (MQ=255) ttGTTCACTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGAGTGTTCGCGATCCAGCGTCTGGTTGa > 2:378732/1‑76 (MQ=255) ttGTTCACTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGAGTGTTCGCGATCCAGCGTCTGGTTGa > 2:210078/1‑76 (MQ=255) tGTTCACTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGAGTGTTCGCGATCCAGCGTCTGGTTGAg > 1:1215736/1‑76 (MQ=255) tGTTCACTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGAGTGTTCGCGATCCAGCGTCTGGTTGAg > 2:377755/1‑76 (MQ=255) tGTTCACTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGACGGAGTGTTAGCGATCCAGCGTCTGGTCGAg > 2:105023/1‑76 (MQ=255) ttCACTTCCAGCAGNTTCAGCCAGCAGCTATGCAGCATCTCGGTGGAGTGTTCGCGATCCTGCCTCTGGTTGAGtt < 1:1477488/76‑1 (MQ=255) ccAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGAGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTc < 1:678133/76‑1 (MQ=255) tCAGCCAGCAGCTATGCAGCATCTCGATGGAGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATa > 1:670903/1‑76 (MQ=255) cagccagcAGCTATGCAGCATCTCGATGGAGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAg > 2:1223501/1‑76 (MQ=255) gccagcAGCTATGCAGCATCTCGATGGAGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:1091509/76‑1 (MQ=255) cagcagCTATGCAGCATCTCGATGGAGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACg < 2:1303800/76‑1 (MQ=255) cagcTATGCAGCATCTCGATGGAGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGt > 1:361897/1‑76 (MQ=255) gcTATGCAGCATCTCGATGNANTGTTCGCNATCCAGCGTCTGGGTGAGTTCNACNTCACGGCGATAGTACGGGTag < 2:248447/76‑1 (MQ=255) cTATGCAGCATCTCGATGGAGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTaga > 1:1025747/1‑76 (MQ=255) aTGCAGCATCTCGATGGAGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGgggaggga > 2:479775/1‑76 (MQ=255) aTGCAGCATCTCGATGGAGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGgagaga > 2:479754/1‑76 (MQ=255) aTGCAGCATCTCGATGGAGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAgaga > 2:515232/1‑76 (MQ=255) aTGCAGCATCTCGATGGAGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAgaga > 2:961931/1‑76 (MQ=255) cagcaTCTCGATGGAGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGCAGAGATAc > 2:79089/1‑76 (MQ=255) agcaTCTCGATGGAGTGTTCGCGATTCACCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACt < 1:260449/76‑1 (MQ=255) gcaTCTCTGTGGTGCGTTCTCGATCTAGCTTCTGGTTGAATTCAACGTCGCGGCGATAGTACGGGTATAGATATTg < 2:34703/76‑1 (MQ=255) caTCTCGATGGAGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTgg > 2:338375/1‑76 (MQ=255) tctcGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTc < 1:1159133/76‑1 (MQ=255) tcGATGGAGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCa < 1:222565/76‑1 (MQ=255) cGATGGAGTGTTCGCTATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCAt < 1:973130/76‑1 (MQ=255) cGATGGAGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCAt > 2:1350299/1‑76 (MQ=255) cGATGGAGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCAt < 1:953783/76‑1 (MQ=255) cGATGGAGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCAt < 2:769406/76‑1 (MQ=255) gATGGAGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATa < 2:1423040/76‑1 (MQ=255) aTGGAGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATAc < 1:1623194/76‑1 (MQ=255) | TTGAGTGTGAGCCGGAGCGGATCTTGTTCACTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATAC > REL606/854153‑854296 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |