| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,400,364 | T→C | D21G (GAT→GGT) | abgB ← | predicted peptidase, aminobenzoyl‑glutamate utilization protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,400,364 | 0 | T | C | 98.0% | 197.6 / ‑3.9 | 50 | D21G (GAT→GGT) | abgB | predicted peptidase, aminobenzoyl‑glutamate utilization protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (23/26); total (24/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
CGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATATCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCATCAGATACCTC > REL606/1400291‑1400435 | cGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCg < 1:835655/76‑1 (MQ=255) aGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGta > 2:431304/1‑76 (MQ=255) cAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATaa > 2:619310/1‑76 (MQ=255) cAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATaa < 1:638728/76‑1 (MQ=255) ataTGCTCCGCTGACCACAACTCATGAAAACGTGTTTCTNGATGATCCCAGATTTNATCGGCAATACCGGTATAAc > 2:1693519/3‑76 (MQ=255) gCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTg > 2:1200627/1‑76 (MQ=255) ccGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCg < 2:912239/76‑1 (MQ=255) ccGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCg < 2:818646/76‑1 (MQ=255) tGACCAGAACTCTTCGAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCg < 1:1415187/76‑1 (MQ=255) gACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCgg > 2:1280939/1‑76 (MQ=255) gACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCgg > 2:733394/1‑76 (MQ=255) gACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCgg > 2:383268/1‑76 (MQ=255) aGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTc < 1:447651/76‑1 (MQ=255) aGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTc < 2:617317/76‑1 (MQ=255) aGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTc < 2:162293/76‑1 (MQ=255) gAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCa < 1:617807/76‑1 (MQ=255) ctctTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATc > 1:196758/1‑76 (MQ=255) tctTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCg > 1:380517/1‑76 (MQ=255) ctTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGc > 2:638284/1‑76 (MQ=255) ttCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCa < 2:933851/76‑1 (MQ=255) tCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCAt > 2:666215/1‑76 (MQ=255) aaaCGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCAtcgt < 1:422511/76‑1 (MQ=255) aaaCGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCAtcgt > 1:286747/1‑76 (MQ=255) aaaCGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCAtcgt < 1:1297006/76‑1 (MQ=255) aCGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCAtcgtcg < 2:646328/76‑1 (MQ=255) gtTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATaa < 1:296464/76‑1 (MQ=255) ttCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATaaaa < 1:765210/76‑1 (MQ=255) ttCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATaaaa > 2:1143450/1‑76 (MQ=255) ttCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATaaaa > 1:1151050/1‑76 (MQ=255) ntGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACg > 1:473979/2‑76 (MQ=255) cTGGATGATCCCAGATTTGATCGGGAATACCGGTATAACGTTGGCGATCGGCTGTACTGGCGTCGTCGATaaaaag > 1:76753/1‑74 (MQ=255) cTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACg > 1:1354511/1‑76 (MQ=255) ggATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGAt < 1:579535/76‑1 (MQ=255) gatgatCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATa < 1:1362448/76‑1 (MQ=255) atgatCCCAGATTTGATCGGCAATATCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAg > 2:747042/1‑76 (MQ=255) atgatCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAg < 1:1353303/76‑1 (MQ=255) tgatCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGa < 1:496756/76‑1 (MQ=255) gatCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGAt > 1:1188898/1‑76 (MQ=255) cccAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTc < 1:1143452/76‑1 (MQ=255) cAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCt < 1:1200629/76‑1 (MQ=255) aTTTGATCGGCAATACCGGTATAACGTTGGCGATCAGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCa < 2:1370849/76‑1 (MQ=255) tttGATCGGCAATACCGGCATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCAt > 1:1003658/1‑76 (MQ=255) ttGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCATc < 2:1421959/76‑1 (MQ=255) tGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCNTGCATCa < 2:1375690/76‑1 (MQ=255) tGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCATCa < 1:1423155/76‑1 (MQ=255) tGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCATCa < 1:765274/76‑1 (MQ=255) tCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCATCAGAt > 1:1205495/1‑76 (MQ=255) cGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCATCAGATa > 2:787513/1‑76 (MQ=255) gCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCATCAGATAcc > 2:1314661/1‑76 (MQ=255) aaTACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCATCAGATACCTc > 2:161582/1‑76 (MQ=255) | CGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATATCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCATCAGATACCTC > REL606/1400291‑1400435 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |