Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,665,436	(A)7→6	coding (405/483 nt)	yfiB →	predicted outer membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,665,430	0	A	.	100.0%	157.1 / NA	32	coding (399/483 nt)	yfiB	predicted outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (20/12);  total (20/12)

GGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGAT  >  REL606/2665355‑2665501                                                                            |                                                                        ggCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGa                                                                         >  1:1801455/1‑76 (MQ=255)  gCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaa                                                                        <  2:1059198/76‑1 (MQ=255)   cGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaa                                                                       >  2:1317861/1‑76 (MQ=255)   cGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaa                                                                       <  1:935393/76‑1 (MQ=255)    gAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaaa                                                                      >  1:1235467/1‑76 (MQ=255)    gAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaaa                                                                      >  1:488550/1‑76 (MQ=255)     aaTGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGTaaaaa                                                                     >  2:55546/1‑76 (MQ=255)     aaTGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaaaa                                                                     >  1:817232/1‑76 (MQ=255)     aaTGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaaaa                                                                     >  2:1769140/1‑76 (MQ=255)     aaTGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaaaa                                                                     >  2:90610/1‑76 (MQ=255)       tGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaaaaat                                                                   <  1:1911796/76‑2 (MQ=255)        gTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaaaanta                                                                  <  2:1395422/76‑4 (MQ=255)        gTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaaaaata                                                                  <  1:1696882/76‑3 (MQ=255)         tCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaaaaatat                                                                 <  1:1809333/76‑4 (MQ=255)             ggCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCa                                                            <  1:1648128/76‑1 (MQ=255)                cGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAg                                                         >  2:1834681/1‑76 (MQ=255)                 gATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTGACCACACAGGGTTTAGG‑AAAAAATATCCCATAGc                                                        <  1:525455/76‑1 (MQ=255)                 gATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGc                                                        >  1:759985/1‑76 (MQ=255)                  aTGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGcc                                                       >  1:1900441/1‑76 (MQ=255)                      aTGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTa                                                   <  2:10014/76‑1 (MQ=255)                      aTGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTa                                                   >  1:72237/1‑76 (MQ=255)                           cTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAg                                              <  2:1021173/76‑1 (MQ=255)                                     aCAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAgg                                    >  1:310235/1‑76 (MQ=255)                                      cAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAggg                                   <  1:1670621/76‑1 (MQ=255)                                       aaaTTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGc                                  >  2:925252/1‑76 (MQ=255)                                         aTTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCcg                                >  2:458493/1‑76 (MQ=255)                                          ttCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCcgc                               >  1:1086328/1‑76 (MQ=255)                                              aCGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCg                           <  1:1669832/76‑1 (MQ=255)                                                gcagcaATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCgag                         >  2:1056938/1‑76 (MQ=255)                                                 cagcaATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCgaga                        <  2:1097654/76‑1 (MQ=255)                                                  agcaATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGaa                       >  2:678824/1‑76 (MQ=255)                                                  agcaATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGaa                       <  1:583571/76‑1 (MQ=255)                                                   gcaATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAAc                      <  1:1558537/76‑1 (MQ=255)                                                    caATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAAcc                     >  1:1481481/1‑76 (MQ=255)                                                     aaTCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAAccg                    >  1:892677/1‑76 (MQ=255)                                                      aTCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAAccgc                   >  1:1267323/1‑76 (MQ=255)                                                       tctcACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAAccgcc                  >  1:271618/1‑76 (MQ=255)                                                       tctcACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAAccgcc                  >  2:1575272/1‑76 (MQ=255)                                                          caccacACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGCCCGCCCAGGGCCGCGCCGAGAACCGCcgcg               <  2:1631859/76‑1 (MQ=255)                                                          caccacACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCcgcg               <  1:114630/76‑1 (MQ=255)                                                          caccacACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCcgcg               >  1:848001/1‑76 (MQ=255)                                                            ccacACGGGGTTAAGG‑AAAAAATACCCCATAGCCAGTAACAAGACCGCCCGGGGCCGCGCCGAGAACCGCCGCGTc             <  1:649400/76‑1 (MQ=255)                                                                  aGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAgtg       >  1:18673/1‑76 (MQ=255)                                                                  aGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAgcg       >  1:1532803/1‑74 (MQ=255)                                                                     gTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAgtggtg    <  1:730049/76‑1 (MQ=255)                                                                      tttAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGa   >  2:1898825/1‑76 (MQ=255)                                                                       ttAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGAt  >  2:788347/1‑76 (MQ=255)                                                                            |                                                                        GGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGAT  >  REL606/2665355‑2665501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑