| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,484,105 | 0 | A | G | 22.7% | 102.0 / 16.0 | 44 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (6/28); new base G (10/0); total (16/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.23e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TACTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAAT > REL606/1484032‑1484180 | tACTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAtt < 1:1019890/76‑1 (MQ=255) aCTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGGCACTGGTATGGCAGGGAgtt > 1:1120673/1‑76 (MQ=255) ctgctgCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAtttt < 1:1063793/76‑1 (MQ=255) gctTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGGCTCTGTTATGGCAGGGATTTTATACt < 1:1389012/76‑1 (MQ=255) gctTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACt < 1:380435/76‑1 (MQ=255) ctTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACtt > 1:1066818/1‑76 (MQ=255) ctTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACtt < 1:78097/76‑1 (MQ=255) ttGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGATCGCTGCTGCCACTGTTATGGCAGGGGGATTTTACttt > 2:1782662/1‑76 (MQ=255) ggCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCttttt > 1:77224/1‑75 (MQ=255) ggCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTTCttttt > 2:1745572/1‑75 (MQ=255) ggCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTg < 2:1281480/76‑1 (MQ=255) ggCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTg < 1:333463/76‑1 (MQ=255) gCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTACtttttt > 1:1240664/1‑74 (MQ=255) ccACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACg < 1:1152984/76‑1 (MQ=255) ccACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACg < 2:616026/76‑1 (MQ=255) tAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGAttt < 1:681079/76‑1 (MQ=255) aaTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTc < 2:1484780/76‑1 (MQ=255) aTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCa < 2:1063545/76‑1 (MQ=255) gtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCAcc < 1:1852642/76‑1 (MQ=255) gtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCAcc < 1:1980106/76‑1 (MQ=255) ggTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCAt < 1:1298352/76‑1 (MQ=255) gTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATg < 1:1137417/76‑1 (MQ=255) tGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTTCGATTTTACCATGTCg > 1:1471330/1‑76 (MQ=255) ttGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAAc < 2:260977/76‑1 (MQ=255) gTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTTTTTTTTTGATTTTCCCATGTCGGGAACGc > 1:739164/1‑76 (MQ=255) cTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCTTTTTTCGATTTTACCCTGTCGGGAAAGCCg > 1:849417/1‑76 (MQ=255) aTTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTTCGATTTTACCATTTCGGGAACGcccgg > 2:178284/1‑73 (MQ=255) aTTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTCACCATGTCGGGAACGcccgg > 2:1253050/1‑73 (MQ=255) aTTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGa < 1:730810/76‑1 (MQ=255) tCGGCTCGCTGCTGACACTGTTATGGCAGGGGTCTTTTACTTTTGACGATTTCACCATGTCGGTAACGCCGGGACt > 2:73662/1‑76 (MQ=255) cTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGTCg < 1:339823/76‑1 (MQ=255) cTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACg < 2:1879700/76‑1 (MQ=255) cTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACg < 2:1791632/76‑1 (MQ=255) cGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCt < 1:903338/76‑1 (MQ=255) gctgctGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGccg > 1:1714209/1‑74 (MQ=255) tgctgACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGc < 1:77953/76‑1 (MQ=255) tgctgACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGc < 1:1464109/76‑1 (MQ=255) tgACACTGTTATGGCAGGGGTTTTTTACTTTTGACGATTTTACCATGTCGGGAACGCCGGAACTGACGCTGGCGaa > 1:308887/1‑76 (MQ=255) acacTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAata < 1:28253/76‑1 (MQ=255) cacTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAatat < 2:123953/76‑1 (MQ=255) acTGTTATGGCAGGGATTTTATACTTTTGACGATTCCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATc < 1:105610/76‑1 (MQ=255) tGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCg < 2:249914/76‑1 (MQ=255) ggCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCt > 1:360577/1‑76 (MQ=255) cAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGt > 2:478902/1‑76 (MQ=255) aGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGtt > 2:1858970/1‑76 (MQ=255) aGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGtt > 1:1463278/1‑76 (MQ=255) gATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTaa > 2:703143/1‑76 (MQ=255) aTTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAAt < 1:1909569/76‑1 (MQ=255) aTTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAAt > 1:1748001/1‑76 (MQ=255) aTTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAAt > 2:527811/1‑76 (MQ=255) | TACTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAAT > REL606/1484032‑1484180 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |