| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,043,426 | 0 | T | A | 24.7% | 183.7 / 21.7 | 74 | I200F (ATT→TTT) | yihO | predited transporter |
| Reads supporting (aligned to +/- strand): ref base T (38/17); new base A (0/18); total (38/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.25e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TACTGGCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAAC > REL606/4043351‑4043499 | tACTGGCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAAt > 2:1042086/1‑76 (MQ=255) aCTGGCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATa > 1:264951/1‑76 (MQ=255) tGGCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATaaa < 2:536214/76‑1 (MQ=255) tGGCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATaaa > 2:1265976/1‑76 (MQ=255) tGGCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATaaa < 1:1566735/76‑1 (MQ=255) gCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAgc > 1:1253847/1‑76 (MQ=255) gCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAgc < 1:951438/76‑1 (MQ=255) gCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAgc < 1:461842/76‑1 (MQ=255) gCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAgc > 1:170369/1‑76 (MQ=255) tATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAgccgcc < 2:1148610/76‑1 (MQ=255) aTGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCa > 2:979184/1‑76 (MQ=255) aTGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCa < 2:31957/76‑1 (MQ=255) gTCGGGTTGGTGTTGTGCCCAAATAATGCTCTTTGCCCCTTTTGAAGCACACCATAAAAAAAAAAAGGCCGCCAAt < 2:1428575/76‑1 (MQ=255) gTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAAt > 2:1352689/1‑76 (MQ=255) gTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAAt > 1:1459609/1‑76 (MQ=255) tCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATg > 2:223242/1‑76 (MQ=255) cgggTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATgg < 2:671498/74‑1 (MQ=255) cgggTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATAATAAAAAAAAAGCCGCCAATgg < 2:976921/74‑1 (MQ=255) ggTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGgag > 1:4407/1‑76 (MQ=255) gTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGgaga > 2:1724240/1‑76 (MQ=255) ttGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGaa < 2:1482212/76‑1 (MQ=255) tGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAAc > 2:1333471/1‑76 (MQ=255) ggTGCTGTGTCCACATAATGCTCTTTGCCGCCTCTGTAGCACAGCATAAAAAAAAAAAAGCCGCCAATGGAGAACa < 1:498264/76‑1 (MQ=255) gTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACAt > 1:1652168/1‑76 (MQ=255) gCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCACTGGAGAACATaa > 2:767599/1‑76 (MQ=255) gCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATaa < 2:450894/76‑1 (MQ=255) cTGTGCCCAAATATTCTTCTTTGCCCCCTCTGTAGCACAGCATAAAAAAAAAAAAGCCGCCAATGGAGAACATAAg < 1:1225625/76‑1 (MQ=255) tgtgTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGt > 1:1862985/1‑76 (MQ=255) tgtgTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGt > 1:1182600/1‑76 (MQ=255) tgtgTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGt > 2:1120511/1‑76 (MQ=255) gtgtCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTg > 2:294936/1‑76 (MQ=255) tgtCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGc > 1:1069567/1‑76 (MQ=255) gtCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGcc > 2:1038024/1‑76 (MQ=255) acaTAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGcac > 1:739809/1‑76 (MQ=255) caTAATGCTCTTTGCCGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGcaca < 2:458988/76‑1 (MQ=255) caTAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGcaca < 2:274167/76‑1 (MQ=255) aTAATGCTCTTTGCCGCCTCTGTAGCACAGCATCAAAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAg < 1:1451806/76‑1 (MQ=255) aTAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAg > 1:332824/1‑76 (MQ=255) tAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAgg > 2:1098039/1‑76 (MQ=255) tAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAgg > 1:957192/1‑76 (MQ=255) aaTGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGc > 2:1937620/1‑76 (MQ=255) aaTGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGc > 2:1431886/1‑76 (MQ=255) aaTCCTCTTTGCCGCCTCTGTAGCACAGCATAAAAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGc < 1:691611/76‑1 (MQ=255) tGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCAt < 2:1918713/76‑1 (MQ=255) tGCTCGTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCAt < 2:1918737/76‑1 (MQ=255) gCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATa > 2:1913175/1‑76 (MQ=255) gCTCTTTGACGCCTCTGTAGCACAGCATCAAAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATa < 1:284858/76‑1 (MQ=255) tctTTGACGCCTCTGTAGCACAGCATCAAAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAAc < 1:1067844/76‑1 (MQ=255) ctTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAAcc < 2:589664/76‑1 (MQ=255) tttGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCg > 1:1302175/1‑76 (MQ=255) tttGACGCCTCTGAAGCACAGCATAAAAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCg < 1:645897/76‑1 (MQ=255) ttGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGa > 2:1103006/1‑76 (MQ=255) ttGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGa > 2:1602010/1‑76 (MQ=255) tGACGCCTCTGTAGCACAGCATAATAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGAc < 2:1565165/76‑1 (MQ=255) gACGCCTCTGTAGCACAGCATAAAAAAAAGAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACg < 2:585011/76‑1 (MQ=255) aCGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACgg > 1:24930/1‑76 (MQ=255) aCGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACgg > 2:600392/1‑76 (MQ=255) ccTCTGTAGCACAGCATCAAAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTg < 2:1551565/76‑1 (MQ=255) ctctGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGa < 2:79047/76‑1 (MQ=255) ctctGTAGCACAGCATCAAAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGa < 2:343311/76‑1 (MQ=255) ctctGTAGCACAGCATAAAAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGa < 1:1662128/76‑1 (MQ=255) tctGTAGCACAGCATCAAAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAg < 2:1417759/76‑1 (MQ=255) ctGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGt > 1:1589184/1‑76 (MQ=255) gaaGCACAGCATCAAAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCa < 2:376157/74‑1 (MQ=255) gTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCa > 1:1693006/1‑76 (MQ=255) tAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAg > 2:1966291/1‑76 (MQ=255) aGCACAGCATAAAAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGa < 1:104235/76‑1 (MQ=255) caGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGaaaaa > 2:907789/1‑76 (MQ=255) aGCATAATAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGaaaaaa < 2:1540678/76‑1 (MQ=255) tcatAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGa > 2:890249/1‑76 (MQ=255) atAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACt < 2:679104/76‑1 (MQ=255) tAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTg > 1:795889/1‑76 (MQ=255) aaaaaaaaaAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTg < 1:355461/75‑1 (MQ=255) aaaaaaTAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTg < 1:403781/75‑1 (MQ=255) aaaaaaaaGCCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGc < 2:1733018/76‑1 (MQ=255) aaaaaaaaAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTgn < 1:361623/76‑2 (MQ=255) aaaaaaaaAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGc < 1:527505/76‑1 (MQ=255) aaaaaTAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGc < 2:1851577/76‑1 (MQ=255) aaaaaTAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGc > 1:187919/1‑76 (MQ=255) aaaTAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCaa > 2:1479385/1‑76 (MQ=255) aaTAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAAc > 2:914032/1‑76 (MQ=255) | TACTGGCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAAC > REL606/4043351‑4043499 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |