| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 474,837 | C→T | E157K (GAA→AAA) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 474,837 | 0 | C | T | 95.9% | 174.5 / ‑2.5 | 49 | E157K (GAA→AAA) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (28/19); total (30/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
CCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAG > REL606/474764‑474909 | cccACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTca > 1:498411/1‑76 (MQ=255) cacaTCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTcaca > 2:709147/1‑76 (MQ=255) cacaTCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTcaca > 1:1692201/1‑76 (MQ=255) acaTCTCCCTTTTCCATCTTCCCTGCCACTCCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACaa < 2:605202/76‑1 (MQ=255) acaTCGCCCTTTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACaa < 2:1273839/76‑1 (MQ=255) caTCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACAAt > 2:551396/1‑76 (MQ=255) cccTGTCCCACCATCCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATcaacc < 1:661762/76‑1 (MQ=255) ccTGTTCCATCATTCTTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATcaacca < 1:432141/76‑1 (MQ=255) ccTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATcaacca > 2:1503972/1‑76 (MQ=255) cTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATcaaccaa > 2:1698272/1‑76 (MQ=255) tGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATcaaccaac > 1:1800032/1‑76 (MQ=255) ttCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCg > 1:1537480/1‑76 (MQ=255) ccATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGAt > 2:1039837/1‑76 (MQ=255) catcatTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATg > 2:372507/1‑76 (MQ=255) tgatTCCTGCCACTGGGGGCAGCAACACCAGCGTCAGAGCCATTGCCAGGTCTTTCACAATCAGCCAACCGATGGc < 1:23043/74‑1 (MQ=255) tcatTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGc > 1:1610303/1‑76 (MQ=255) catTCCTGCCACTGCGGGCAGCAACACCAGCGTCGGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCg > 1:1773223/1‑76 (MQ=255) catTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTGCCAGGTCTTTCACAATCAACCAACCGATGGCg < 1:228115/76‑1 (MQ=255) catTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCg > 2:1210859/1‑76 (MQ=255) atTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGa > 1:1877038/1‑76 (MQ=255) atTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGa > 2:524974/1‑76 (MQ=255) cccTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGAtt < 1:763079/75‑1 (MQ=255) cccTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGAtt < 1:937878/75‑1 (MQ=255) ccTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGAttt < 2:1492597/76‑1 (MQ=255) ctcccACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGATTTg < 2:877638/73‑1 (MQ=255) tGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGc > 2:232641/1‑76 (MQ=255) gCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGATTTGcc > 2:1597885/1‑76 (MQ=255) gCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGATTTGcc > 2:376645/1‑76 (MQ=255) gCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGATTTGcc > 2:668628/1‑76 (MQ=255) gggCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGATTTGCCCACGCTGa > 1:367746/1‑76 (MQ=255) cagcaACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTg > 1:197320/1‑76 (MQ=255) caACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCa > 2:183233/1‑76 (MQ=255) caACACCAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCa > 1:843823/1‑76 (MQ=255) acacCAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCaat < 1:182064/76‑1 (MQ=255) cccAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCaatta < 1:1148009/75‑1 (MQ=255) acCAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCaatta < 2:1087875/76‑1 (MQ=255) ccAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCaattaa < 2:942495/76‑1 (MQ=255) cAGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCaattaat < 1:1643037/76‑1 (MQ=255) aGCGTCAGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCaattaatt < 2:860980/76‑1 (MQ=255) aGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGtt > 2:1517054/1‑76 (MQ=255) aGAACCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGtt > 1:1599146/1‑76 (MQ=255) aCCATTACCAGGTCTTTCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGttctt < 2:1507046/76‑1 (MQ=255) aTTACCAGGTCTTTCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCaa > 1:1462790/1‑76 (MQ=255) aTTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCaa > 1:279742/1‑76 (MQ=255) ttACCAGGTCTTTCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAg > 2:280565/1‑76 (MQ=255) tACCAGGTCTTTCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGt > 1:1850437/1‑76 (MQ=255) ccAGGTCTTTCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTgc > 1:987213/1‑76 (MQ=255) ccAGGTCTTTCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTgc < 1:709147/76‑1 (MQ=255) cAGGTCTTTCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTgcg < 2:340892/76‑1 (MQ=255) cTTTCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAg < 1:1125347/76‑1 (MQ=255) | CCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAG > REL606/474764‑474909 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |