| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,202,382 | T→G | T60P (ACC→CCC) | iclR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,202,382 | 0 | T | G | 98.3% | 239.4 / ‑4.3 | 58 | T60P (ACC→CCC) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (31/26); total (32/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.59e-01 | |||||||||||
TGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCC > REL606/4202311‑4202453 | tGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTg > 1:874446/1‑76 (MQ=255) gCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTgg < 1:1886789/76‑1 (MQ=255) cccAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGa > 2:883422/1‑76 (MQ=255) cccAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGa > 2:294532/1‑76 (MQ=255) cAATGTCCCAGTTCGCCGACCTGACGCAGGAAACCCTGCTGTTGCATTGCGGTTAGCAGGCGGTGGGGCggggggt > 2:1850345/1‑73 (MQ=255) cAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAAt < 2:1648290/76‑1 (MQ=255) cAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAAt > 2:1982030/1‑76 (MQ=255) cAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAAt < 1:140989/76‑1 (MQ=255) cAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAAt > 2:684325/1‑76 (MQ=255) tGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTgg < 1:1215704/76‑1 (MQ=255) tGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTgg < 2:354227/76‑1 (MQ=255) tCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGt > 2:1346154/1‑76 (MQ=255) cccAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTa > 1:1798899/1‑76 (MQ=255) ccAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTaa < 2:1951202/76‑1 (MQ=255) ccAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTaa < 2:462787/76‑1 (MQ=255) ccAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTaa < 2:63670/76‑1 (MQ=255) aGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAAcc < 2:261469/76‑1 (MQ=255) gTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGCGGGGCGGGGGATTGGGGAAccc > 1:936532/1‑76 (MQ=255) gCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCt < 2:937913/76‑1 (MQ=255) ccGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCtt < 1:490207/76‑1 (MQ=255) gccTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCttgtt < 2:1457093/75‑1 (MQ=255) aCCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCttgtt < 2:1288622/76‑1 (MQ=255) tGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTgcg > 1:446271/1‑76 (MQ=255) tGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTgcg > 1:730167/1‑76 (MQ=255) tGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTgcg < 2:913836/76‑1 (MQ=255) gACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTgcgc > 1:709708/1‑76 (MQ=255) cGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCa < 1:1118225/76‑1 (MQ=255) cGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCa < 1:130062/76‑1 (MQ=255) cACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGt > 2:844020/1‑76 (MQ=255) cGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTc > 1:767867/1‑76 (MQ=255) cGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTc < 2:1770632/76‑1 (MQ=255) aaaCCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCg > 2:209322/1‑76 (MQ=255) aaaCCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCg > 2:1206077/1‑76 (MQ=255) aaaCCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCg > 2:931208/1‑76 (MQ=255) aaaCCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACACGGCTTGTTGCGCCAGTTCCg > 2:1184102/1‑76 (MQ=255) aaaCCCTGCTGTTGCATTGAGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCg > 2:1114411/1‑76 (MQ=255) aaCCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGt < 1:1818976/76‑1 (MQ=255) tgTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCGGTTCCGTGAGTGCca > 1:1811769/1‑76 (MQ=255) tgTTGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCca > 1:1776391/1‑76 (MQ=255) ttGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCcaca > 1:1130366/1‑76 (MQ=255) gtGCATTGTGGTTAGCAGGCGGTGGGGCGTGGAAGTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCcaca < 2:204571/75‑1 (MQ=255) gCATTGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACt < 2:696482/76‑1 (MQ=255) tgtgGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCa < 2:284596/76‑1 (MQ=255) gtgGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCAt > 1:124192/1‑76 (MQ=255) tgGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCAtt < 2:1239560/76‑1 (MQ=255) tgGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCAtt > 1:1679845/1‑76 (MQ=255) gTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTgg > 1:1387386/1‑76 (MQ=255) gTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTgg < 2:75662/76‑1 (MQ=255) ttAGCAGGCGGTGGGGCGTGGAATTGGGTAGCCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGa < 1:1786632/76‑1 (MQ=255) ttAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGa > 1:577182/1‑76 (MQ=255) tgaGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGa < 1:1849894/74‑1 (MQ=255) tAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGAt > 2:1967076/1‑76 (MQ=255) tAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGAt > 1:120333/1‑76 (MQ=255) aGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGAtt > 1:710137/1‑76 (MQ=255) gCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTc < 2:1920705/76‑1 (MQ=255) cggCCGTGGGGCCTTGAATTAGGGTACCCGGCTTGTTGGGGCAGTTCCGGGGAGGGCGCACTGCCATTGGATTCgg < 2:450158/75‑1 (MQ=255) aGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCgg > 1:228143/1‑76 (MQ=255) aGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCgg > 2:620940/1‑76 (MQ=255) aGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCgg > 2:1176146/1‑76 (MQ=255) ggCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGc < 2:726216/76‑1 (MQ=255) cGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCaa > 1:1798183/1‑76 (MQ=255) ggTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAAt > 2:902335/1‑76 (MQ=255) gTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATc > 2:293215/1‑76 (MQ=255) tGGGGCGTGGAATTGGGTACCCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATcc < 1:685571/76‑1 (MQ=255) tGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATcc > 2:49230/1‑76 (MQ=255) | TGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCC > REL606/4202311‑4202453 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |