Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,358,409	T→A	I24N (ATC→AAC)	pta →	phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,358,409	0	T	A	100.0%	332.7 / NA	87	I24N (ATC→AAC)	pta	phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (47/40);  total (47/40)

GTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGA  >  REL606/2358339‑2358481                                                                       |                                                                         gtgtCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTg                                                                     <  2:674969/76‑1 (MQ=255) gtgtCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTg                                                                     <  1:96697/76‑1 (MQ=255)  tgtCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGc                                                                    >  1:1804943/1‑76 (MQ=255)  tgtCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGCCGGACCAGCGCCAGCCTTGGCGTGAACCGGGc                                                                    >  1:1251720/1‑76 (MQ=255)  tgtCCCGTATTATTATGCTGATCCCTACCCGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGc                                                                    >  1:1691360/1‑76 (MQ=255)   gtCCCGTATTATTATGCTGATCCCTGCCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCa                                                                   <  1:945008/76‑1 (MQ=255)   gtCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCa                                                                   >  1:1672833/1‑76 (MQ=255)   gtCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCa                                                                   <  1:365984/76‑1 (MQ=255)       cGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATgg                                                               <  2:1324262/76‑1 (MQ=255)         tattattatGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGaa                                                             >  2:1230688/1‑76 (MQ=255)          attattatGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAAc                                                            >  1:1214444/1‑76 (MQ=255)           ttattatGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACg                                                           <  1:110987/76‑1 (MQ=255)           ttattatGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACg                                                           >  1:739847/1‑76 (MQ=255)            tattatGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGc                                                          >  1:361461/1‑76 (MQ=255)              ttatGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCaa                                                        <  1:1034513/76‑1 (MQ=255)              ttatGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCaa                                                        >  2:1347505/1‑76 (MQ=255)                atGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAg                                                      >  1:1705370/1‑76 (MQ=255)                atGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAg                                                      >  1:494246/1‑76 (MQ=255)                     gATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGtt                                                 >  2:671815/1‑76 (MQ=255)                     gATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGtt                                                 <  1:679355/76‑1 (MQ=255)                     gATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGtt                                                 >  2:1642141/1‑76 (MQ=255)                      aTCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTc                                                >  1:571911/1‑76 (MQ=255)                      aTCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTc                                                <  1:41851/76‑1 (MQ=255)                       tCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCg                                               >  2:55879/1‑76 (MQ=255)                         ccTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTc                                             >  2:874002/1‑76 (MQ=255)                          cTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCt                                            >  2:1359617/1‑76 (MQ=255)                          cTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCt                                            <  2:661684/76‑1 (MQ=255)                           tACCGGAACCCGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTg                                           >  2:818010/1‑76 (MQ=255)                           tACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTg                                           >  2:1194071/1‑76 (MQ=255)                           tACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTg                                           <  2:196980/76‑1 (MQ=255)                           tACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTg                                           <  2:1010921/76‑1 (MQ=255)                           acccGTAACCAGCGTCGGTCTGACCGGCGTCAGCCTTGGCGTTAACCGTGCAATGGAACGCAAAGGCGTTCGTCTg                                           <  2:1054327/74‑1 (MQ=255)                            aCCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGa                                          >  2:6033/1‑76 (MQ=255)                             cctgAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAg                                         <  2:1256458/73‑1 (MQ=255)                             ccGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAg                                         >  2:1748984/1‑76 (MQ=255)                             ccGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAg                                         <  1:64471/76‑1 (MQ=255)                                gAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGt                                      >  2:249229/1‑76 (MQ=255)                                 agccAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGtt                                     <  2:781390/74‑1 (MQ=255)                                 aaCCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGtt                                     <  1:1775436/76‑1 (MQ=255)                                 aaCCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGtt                                     >  2:1211439/1‑76 (MQ=255)                                 aaCCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGtt                                     >  2:416917/1‑76 (MQ=255)                                  aCCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGttt                                    >  2:1512421/1‑76 (MQ=255)                                  aCCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGttt                                    <  2:949648/76‑1 (MQ=255)                                    cAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTc                                  >  2:470427/1‑76 (MQ=255)                                    cAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTc                                  <  1:1777899/76‑1 (MQ=255)                                    cAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTc                                  >  2:357416/1‑76 (MQ=255)                                    cAGCGTCGGTCTGACCAGCGTCAGCCGTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTc                                  <  2:1608121/76‑1 (MQ=255)                                     aGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCa                                 <  1:1288889/76‑1 (MQ=255)                                     aGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCa                                 <  1:1305305/76‑1 (MQ=255)                                     aGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCa                                 <  2:1088558/76‑1 (MQ=255)                                       cGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCaaa                               >  2:1522976/1‑76 (MQ=255)                                       cGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCaaa                               >  2:1583333/1‑76 (MQ=255)                                        gTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAAc                              <  1:1765094/76‑1 (MQ=255)                                          cGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAAccc                            >  2:565758/1‑75 (MQ=255)                                           ggTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTa                           >  1:1645858/1‑76 (MQ=255)                                           ggTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTa                           <  1:525659/76‑1 (MQ=255)                                           ggTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTa                           >  1:1939081/1‑76 (MQ=255)                                           cgTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTa                           <  2:1645226/75‑1 (MQ=255)                                            gTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTAt                          >  1:344366/1‑76 (MQ=255)                                               tGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGc                       <  1:1377420/76‑1 (MQ=255)                                                gACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCt                      >  2:1377446/1‑76 (MQ=255)                                                gACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCt                      >  2:1029011/1‑76 (MQ=255)                                                gACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCt                      >  2:823553/1‑76 (MQ=255)                                                gACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCt                      >  2:269005/1‑76 (MQ=255)                                                 aCCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTc                     >  2:827133/1‑76 (MQ=255)                                                  ccAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCa                    <  1:1800064/76‑1 (MQ=255)                                                  ccAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCa                    <  2:191063/76‑1 (MQ=255)                                                     gCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGcc                 <  1:1032165/76‑1 (MQ=255)                                                     gCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGcc                 <  1:1388688/76‑1 (MQ=255)                                                      cGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCaaa                               <  2:297299/61‑1 (MQ=255)                                                      cGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCaaa                               >  1:297299/1‑61 (MQ=255)                                                      cGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGcca                >  1:1435088/1‑75 (MQ=255)                                                      cGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCcg                <  2:1804942/76‑1 (MQ=255)                                                      cGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCcg                <  2:1691360/76‑1 (MQ=255)                                                      cGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCcg                <  2:1251720/76‑1 (MQ=255)                                                       gTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCcgc               <  2:213323/76‑1 (MQ=255)                                                        tCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCcgcn              <  1:590547/76‑2 (MQ=255)                                                        tCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCcgcg              <  2:534588/76‑1 (MQ=255)                                                        tCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCcgcg              >  2:1850303/1‑76 (MQ=255)                                                          aGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTa            >  2:1465733/1‑76 (MQ=255)                                                            ccTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTAcc          >  2:713552/1‑76 (MQ=255)                                                             cTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCg         >  2:327744/1‑76 (MQ=255)                                                               tGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGt       >  2:721727/1‑76 (MQ=255)                                                                ggCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTAGCGCTCAGCCGCGTACCGGTg      <  1:588911/76‑1 (MQ=255)                                                                  cGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGc    <  2:162092/76‑1 (MQ=255)                                                                   gTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCg   <  2:988741/76‑1 (MQ=255)                                                                    tGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAACCCTATCGCTCAGCCGCGTACCGGTGGCGa  >  2:66903/1‑76 (MQ=255)                                                                    tGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGa  >  2:86035/1‑76 (MQ=255)                                                                    tGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGAGCCGa  >  2:1880270/1‑76 (MQ=255)                                                                       |                                                                         GTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGA  >  REL606/2358339‑2358481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑