| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,296,329 | 0 | A | C | 39.5% | 67.0 / 19.9 | 43 | intergenic (‑149/‑66) | inaA/yfaH | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (22/4); new base C (0/17); total (22/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.42e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCGTATTAATGAATTTCTTTTGCCACTACTAGCGTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAATTTAATCAGTCGGATACAGCGATGAAGTTT > REL606/2296255‑2296403 | tCGTATTAATGAATTTCTTTTGCCACTACTAGCGTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTcca > 2:365784/1‑75 (MQ=255) cGTATTAATGAATTTCTTTTGCCACTACTAGCGTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTccac > 2:88448/1‑75 (MQ=255) cGTATTAATGAATTTCTTTTGCCACTACTAGCGTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTccac < 1:1001447/75‑1 (MQ=255) gTATTAATGAATTTCTTTTGCCACTACTAGCGTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTccacc < 1:1291477/75‑1 (MQ=255) ttAATGAATTTCTTTTGCCACTACTAGCGTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGc > 1:874901/1‑75 (MQ=255) tAATGAATTTCTTTTGCCACTACTAGCGTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGcc < 2:724519/75‑1 (MQ=255) tAATGAATTTCTTTTGCCACTACTAGCGTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGcc > 2:1307580/1‑75 (MQ=255) gAATTTCTTTTGCCACTACTGGCGTGACACCGTTTTTCCCCTTCATTTGCAGCGCATAGATCCCACCAGccacta < 2:157685/75‑1 (MQ=255) gAATTTCTTTTGCCACTACTAGCGTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGccacta > 2:1002921/1‑75 (MQ=255) ttcccACTACTAGCGTGACCCCGCTTTTCCCCTTCATTTGCAGCGCATAGATCCCCCCAGCCACTACCACTAATa < 2:1005495/72‑1 (MQ=255) ttGCCACTACTAGCGTGACCCCGCTTTTCCCCTTCATTTGCAGCGCATAGATTCCCCCAGCCACTACCACTAATa < 2:876532/75‑1 (MQ=255) gCCACTACTAGCGTGACACCGCTTTTCCCCTTCATTGGCAGCGCATAGATTCCCCCAGCCACTACCACTAATAGt < 2:1531039/75‑1 (MQ=255) ccACTACTAGCGTGCCCCCGCTTTTCCCCTTCATTTGCAGCGCATAGATCCCCCCAGCCACTACCACTAATAGTg < 1:501304/75‑1 (MQ=255) cACTACTAGCGTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTgc > 2:700239/1‑75 (MQ=255) tactaGCGTGCCCCCGCTTTTCCCCTTCATTTGCAGCGCATAGATTCCCCCAGCCACTACCACTAATAGTGCGcc < 1:1307581/75‑1 (MQ=255) tactaGCGTGACCCCGCTTTTCCCCTTCATTGGCAGCGCATAGATCCCCCCAGCCACTACCACTAATAGTGCGcc < 1:993682/75‑1 (MQ=255) tactaGCGTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGcc > 2:718345/1‑75 (MQ=255) tactaGCGTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGcc > 1:899291/1‑75 (MQ=255) actaGCGTGACCCCTCTTTTCCCCTTCATTTGCAGCGCATAGATCCCCCCAGCCACTACCACTAATAGTGCGccc < 2:340476/75‑1 (MQ=255) actaGCGTGACCCCGCTTTTCCCCTTCATTGGCAGCGCATAGATTCCCCCAGCCACTACCACTAATAGTGCGccc < 1:1540628/75‑1 (MQ=255) actaGCGTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGccc > 2:1576991/1‑75 (MQ=255) ctaGCGTGCCCCCGTTTTTCCCCTTCATTTGCAGCGCATAGATTCCCCCAGCCACTACCACTAATAGTGCGCCCa < 1:998738/75‑1 (MQ=255) ctaGCGTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCa > 2:523318/1‑75 (MQ=255) ctaGCGTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCa > 2:1104765/1‑75 (MQ=255) taGCGTGACCCCGCTTTTCCCCTTCATTTGCAGCGCATAGATTCCCCCAGCCACTACCACTAATAGTGCGCCCAg < 2:1538083/75‑1 (MQ=255) taGCGTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAg > 1:489787/1‑75 (MQ=255) tcGTGACTCTGTTTTTCCCTTTAATTTGCAGGCCATATTTTCCCCCAGCCACTACCACTAATAGTGCGCCCAGTa < 1:356233/74‑1 (MQ=255) gCGTGACCCCGCTTTTCCCCTTCATTGGCAGCGCATAGATTCCCCCAGCCACTACCACTAATAGTGCGCCCAGTa < 2:1039047/75‑1 (MQ=255) cGTCACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGTAg > 1:290540/1‑75 (MQ=255) tGACCCCGCTTTTCCCCTTCATTTGCAGCGCATAGATCCCCCCAGCCACTACCACTAATAGTGCGCCCAGTAGAt < 1:769126/75‑1 (MQ=255) accccGCTTTTACCCTTCATTTGCAGCGCATAGATTCCCCCAGCCACTACCACTAATAGTGCGCCCAGTAGATaa < 1:1276425/72‑1 (MQ=255) acacCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGTAGATaa > 1:118498/1‑75 (MQ=255) cacCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGTAGATaaa > 2:1454071/1‑75 (MQ=255) tccccTTCTTTTGCACCGAATAGATCCCCCCAGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAAtt < 2:108597/73‑1 (MQ=255) tccccTTCATTAGCGGCGCACAGCTTCCACCAGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAAtt < 1:8146/73‑1 (MQ=255) cccTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAATTTa > 1:408243/1‑75 (MQ=255) cccTTCATTTGCAGCGCATAGATCCCCCCAGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAATTTa < 2:1048203/75‑1 (MQ=255) ccTTCATTTGCAGCGCATAGATTCCCCCAGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAATTTaa < 1:1507939/75‑1 (MQ=255) ccTTCATTTGCAGCGCATAGATCCCACCAGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAATTTaa < 2:1164352/75‑1 (MQ=255) cATTGGCAGCGCATAGATTCCCCCAGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAATTTAATCAg < 1:640634/75‑1 (MQ=255) aTTTGCAGCGCATAGTTCCCCCCAGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAATTTAATCAGt < 1:126756/75‑1 (MQ=255) tttGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAATTTAATCAGTc > 2:1176689/1‑75 (MQ=255) ttGCAGCGCATAGATTCCACCAGCCCCTACCACTCATAGTGCGCCCAGTAGATCAAATGTGCATTTAATCAGTCg > 2:1048222/1‑75 (MQ=255) ttGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAATTTAATCAGTCg > 2:1048213/1‑75 (MQ=255) gCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAATTTAATCAGTCGGa > 1:644305/1‑75 (MQ=255) cAGCGCATAATTCCCCCCAGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAATTTAATCAGTCGGAt < 2:671564/75‑1 (MQ=255) gcgcATAGATCCCCCCAGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAATTTAATCAGTCGGATAc < 2:727497/75‑1 (MQ=255) ccATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAATTTAATCAGTCGGATACAg < 1:1395411/74‑1 (MQ=255) aGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAATTTAATCAGTCGGATACAGCGAt > 2:328353/1‑75 (MQ=255) aGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAATTTAATCAGTCGGATACAGCGAt > 1:377775/1‑75 (MQ=255) gATTCCACCAGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAATTTAATCAGTCGGATACAGCGATg > 1:352657/1‑75 (MQ=255) ttCCACCAGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAATTTAATCAGTCGGATACAGCGATGaa > 1:361076/1‑75 (MQ=255) ccactaGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAATTTAATCAGTCGGATACAGCGATGAAGt < 2:1545547/75‑1 (MQ=255) caccaGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAATTTAATCAGTCGGCTACAGCGATGCAGtt > 2:13759/1‑75 (MQ=255) accaGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAATTTAATCAGTCGGATACAGCGATGAAGttt > 1:988587/1‑75 (MQ=255) | TCGTATTAATGAATTTCTTTTGCCACTACTAGCGTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGTAGATAAAATGTGAATTTAATCAGTCGGATACAGCGATGAAGTTT > REL606/2296255‑2296403 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |