| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 474,455 | A→G | L284P (CTG→CCG) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 474,455 | 0 | A | G | 95.8% | 167.4 / ‑2.8 | 48 | L284P (CTG→CCG) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (31/15); total (31/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
GTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAG > REL606/474383‑474525 | gTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACGGc > 2:528313/1‑75 (MQ=255) tCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACGGCa < 2:1093633/75‑1 (MQ=255) cAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACGGCACCGc > 1:1590312/1‑75 (MQ=255) gCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACGGCACCGCaa < 2:1043431/75‑1 (MQ=255) gCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACGGCACCGCaa > 1:1507796/1‑75 (MQ=255) cACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACGGCACCGCaaa < 2:315468/75‑1 (MQ=255) cACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACGGCACCGCaaa < 1:425842/75‑1 (MQ=255) cTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACGGCACCGCAAAcg > 2:740159/1‑75 (MQ=255) gCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACGGCACCGCAAAcgcg < 2:529902/75‑1 (MQ=255) ccAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACGGCACCGCAAACGCGt > 1:1526503/1‑75 (MQ=255) aGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACGGCACCGCAAACGCGTcg > 2:1449439/1‑75 (MQ=255) gTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCa < 1:894417/75‑1 (MQ=255) tGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAAt > 1:771673/1‑75 (MQ=255) gCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATg < 2:415966/75‑1 (MQ=255) gAATCAGAATTAACGGATCAAACAACAGCCCGACGGAGACAAAAAACGGCGCCGCAAACGCGTCGCGCAATGGCa < 2:317142/75‑1 (MQ=255) aaTCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAg > 1:120344/1‑75 (MQ=255) aTCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGc < 2:1150584/75‑1 (MQ=255) aTCAGAATTAACCGATAAAACAACATCCCGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGc < 2:726225/75‑1 (MQ=255) aGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTa > 1:857304/1‑75 (MQ=255) aGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTa > 2:361319/1‑75 (MQ=255) gAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTAt > 1:1448442/1‑75 (MQ=255) tAACGGATCAAACAACATCCCGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTg > 1:56206/1‑75 (MQ=255) aaCGGATCAAACAACATCCCGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTgg > 1:1424875/1‑75 (MQ=255) aCGGATCAAACTACATCCCGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTggg > 2:497203/1‑75 (MQ=255) aCGGATCAAACAACATCCCGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTggg > 1:445325/1‑75 (MQ=255) cAAACAACATCCCGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGggcggcgg > 1:37574/1‑73 (MQ=255) cAAACAACATCCCGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCAc < 2:64943/75‑1 (MQ=255) aaaCAACATCCCGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACg > 1:1450402/1‑75 (MQ=255) aacaacATCCCGACGGAGACAAAACACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACgg > 2:803940/1‑75 (MQ=255) caTCCCGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCCCAGGGACt > 1:1603160/1‑75 (MQ=255) aTCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTc < 1:217050/75‑1 (MQ=255) cccGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAg < 1:672435/75‑1 (MQ=255) cccGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAg < 2:1228629/75‑1 (MQ=255) ccGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGt < 1:765589/75‑1 (MQ=255) ccGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGt < 2:1058731/75‑1 (MQ=255) ccGACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGGATCGTGGGCGGCACGGGGACTCAgg > 1:399389/1‑74 (MQ=255) cGACGGAGACAAAAAACGGTCCCGCAAACGCGGCGCGCAAAGGCTGCGTATCGTGGGCGGCACGGTGACTCAGtt < 2:826698/75‑1 (MQ=255) gACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTc > 2:270291/1‑75 (MQ=255) gACGGAGACAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGCGACTCAGTTc > 1:1584496/1‑75 (MQ=255) agaCAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGTGCGGCACGGGGACTCAGTTCAGACt > 1:771552/1‑75 (MQ=255) agaCAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACt > 1:1481054/1‑75 (MQ=255) gaCAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTc > 1:676411/1‑75 (MQ=255) aCAAAAAACGGCTCCGCAAGCGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCg < 1:1535035/75‑1 (MQ=255) cAAAAAACGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGt > 1:1092714/1‑75 (MQ=255) aaaaaaCAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGtt < 1:59914/75‑1 (MQ=255) aaaaaCGGCGCCGCAACCGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGGCTCAGTTCAGACTCGTTc < 2:643600/75‑1 (MQ=255) aaaaaCGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTc < 1:733834/75‑1 (MQ=255) aaaaaCGGCACCGCAAACGCGGCGCGCAATGGCAGCGTATCGGGGGCGGCACGGTGACTCAGTTCAGACTCGTTc > 1:955728/1‑75 (MQ=255) aaaaCGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCa > 1:1598968/1‑75 (MQ=255) aaaaCGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCa > 1:715214/1‑75 (MQ=255) aaaaCGGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCa > 1:1246688/1‑75 (MQ=255) aaaCGGCACCGCAAGCGCGTCGAGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAg < 1:1392127/75‑1 (MQ=255) aaaCGGCACCGCAAACGCGTCGCGCAATGGCAGGGTATCGTGGGCGGCACGGGGACTCAGTTCAGAGTCGTTCAg > 2:1607444/1‑75 (MQ=255) | GTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAG > REL606/474383‑474525 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |