| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,465,147 | T→A | D41V (GAT→GTT) | ompR ← | osmolarity response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,465,147 | 0 | T | A | 96.8% | 245.8 / ‑3.8 | 62 | D41V (GAT→GTT) | ompR | osmolarity response regulator |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base A (31/29); total (32/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCG > REL606/3465078‑3465216 | aaGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATc < 2:1521750/75‑1 (MQ=255) ccATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGt < 1:798867/75‑1 (MQ=255) aTCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTc < 2:623493/75‑1 (MQ=255) tCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCt > 1:1148648/1‑75 (MQ=255) tCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCt < 1:768622/75‑1 (MQ=255) tCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCt > 1:1397297/1‑75 (MQ=255) ttCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGc > 2:1078926/1‑75 (MQ=255) aCCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCAtt < 1:8771/75‑1 (MQ=255) ccAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTa > 2:1369246/1‑75 (MQ=255) ccAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTa > 2:264637/1‑75 (MQ=255) cAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAg < 1:891834/75‑1 (MQ=255) aGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGc > 2:1538166/1‑75 (MQ=255) aGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGc > 1:520684/1‑75 (MQ=255) aGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGc > 2:703547/1‑75 (MQ=255) tAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGAc < 1:819755/75‑1 (MQ=255) aCATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGc > 1:512467/1‑75 (MQ=255) aTTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCtt > 2:333652/1‑75 (MQ=255) aTTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCtt > 2:333663/1‑75 (MQ=255) ttAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTc < 2:1148648/75‑1 (MQ=255) ttAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTc > 1:498381/1‑75 (MQ=255) aaaTCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGa < 1:134213/75‑1 (MQ=255) aaaTCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGa < 2:876765/75‑1 (MQ=255) aaTCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGaa > 1:971047/1‑75 (MQ=255) aaTCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGaa < 1:670148/75‑1 (MQ=255) aaTCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGaa < 1:1086256/75‑1 (MQ=255) aTCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAAc > 1:1096123/1‑75 (MQ=255) tCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAAcc < 1:1185947/75‑1 (MQ=255) ccACTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCt < 2:1461423/75‑1 (MQ=255) aGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTgg < 2:1379714/75‑1 (MQ=255) gTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGa > 1:1500382/1‑75 (MQ=255) tcccAGAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGaa < 2:614412/73‑1 (MQ=255) tACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGaa < 1:1371517/75‑1 (MQ=255) ccATAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGc > 2:485601/1‑75 (MQ=255) aTAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCt > 1:794727/1‑75 (MQ=255) aTAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCt > 2:597891/1‑75 (MQ=255) tAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCtt < 2:819016/75‑1 (MQ=255) tAAGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCtt > 1:645430/1‑75 (MQ=255) aGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGt < 1:840668/75‑1 (MQ=255) aGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGt < 2:222817/75‑1 (MQ=255) aGATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGt > 2:1510542/1‑75 (MQ=255) gATGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGtt < 1:600698/75‑1 (MQ=255) tGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCg < 1:1403882/75‑1 (MQ=255) tGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCg > 1:571069/1‑75 (MQ=255) tGGAAAGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCg > 2:1599918/1‑75 (MQ=255) aGATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTgag > 1:1297155/1‑75 (MQ=255) gATTCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTgaga > 1:992437/1‑75 (MQ=255) ttCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATa < 2:964481/75‑1 (MQ=255) ttCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATa > 1:788385/1‑75 (MQ=255) ttCACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATa < 2:1186051/75‑1 (MQ=255) tgcACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATa < 1:166679/73‑1 (MQ=255) cACGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAAc > 1:39804/1‑75 (MQ=255) cGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGt > 1:1587630/1‑75 (MQ=255) cGAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGt > 2:1311927/1‑75 (MQ=255) gAGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGtt > 1:536027/1‑75 (MQ=255) aGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTc > 2:1501705/1‑75 (MQ=255) aGTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTc < 1:964751/75‑1 (MQ=255) gTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTcc > 1:171397/1‑75 (MQ=255) gTCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTcc < 2:1110584/75‑1 (MQ=255) tCAGCAGGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCca < 2:498487/75‑1 (MQ=255) gcagGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCcagca > 1:43462/1‑75 (MQ=255) cagGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCcagcag < 1:1458672/75‑1 (MQ=255) cagGCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCcagcag < 1:787070/75‑1 (MQ=255) ggCGAACCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAgcg < 2:1285978/75‑1 (MQ=255) | AAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCG > REL606/3465078‑3465216 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |