| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,619,387 | C→A | intergenic (‑5/+20) | tadA ← / ← yfhB | tRNA‑specific adenosine deaminase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,619,387 | 0 | C | A | 97.1% | 129.2 / ‑3.0 | 35 | intergenic (‑5/+20) | tadA/yfhB | tRNA‑specific adenosine deaminase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (16/18); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01 | |||||||||||
TCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGA > REL606/2619316‑2619458 | tCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATAAACg < 2:1314857/75‑1 (MQ=255)tCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATAAACg > 1:78452/1‑75 (MQ=255) ccAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATAAACgg < 2:14135/75‑1 (MQ=255) cAGTATTCGTAGCTAAACTCGACATCAGACAAAAAGAACACTCCGGAAATAAAAGCGCGGCGCATTATAAACGGa < 2:175581/75‑1 (MQ=255) ttCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATAAACGGACGCTa > 1:1048924/1‑75 (MQ=255) ttCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATAAACGGACGCTa > 1:1196985/1‑75 (MQ=255) ttCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATAAACGGACGCTa < 1:852141/75‑1 (MQ=255) ttCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATAAACGGACGCTa > 1:299107/1‑75 (MQ=255) cTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATAAACGGACGCTATGCTTTa > 2:513431/1‑75 (MQ=255) cTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATAAACGGACGCTATGCTTTa < 1:733702/75‑1 (MQ=255) tCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATAAACGGACGCTATGCTTTACTCTAt > 1:795666/1‑75 (MQ=255) cGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATAAACGGACGCTATGCTTTACTCTAtt < 2:480444/75‑1 (MQ=255) aCTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATAAACGGACGCTATGCTTTACTCTATTcc > 2:430176/1‑75 (MQ=255) aCTTCAGACAAAAAGAAAACTCCGGTTAT‑AAAGCGCGGCGCATTATAAACGGACGCTATGCTTTACTCTATTCCa < 1:922418/75‑1 (MQ=255) cAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATAAACGGACGCTATGCTTTACTCGATTCCAGtt < 2:635569/75‑1 (MQ=255) aCAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATAAACGGACGCTATGCTTTACTCTATTCCAGTTGCt < 1:244106/75‑1 (MQ=255) cAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATAAACGGACGCTATGCTTTACTCTATTCCAGTTGCTg < 1:1171277/75‑1 (MQ=255) gAAAACTCCGGTTATAAAAGCGCGGCGCATTATAAACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTc < 1:179533/75‑1 (MQ=255) gAAAACTCCGGTTATAAAAGCGCGGCGCATTATAAACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTc > 2:990158/1‑75 (MQ=255) cGGTTATAAAAGCGCGGCGCATTATAAACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACcgcgcg > 1:1288666/1‑75 (MQ=255) ggTTATAAAAGCGCGGCGCATTATAAACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCgg < 1:1305715/75‑1 (MQ=255) ttATAAAAGCGCGGCGCATTATAAACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCgggg > 1:624100/1‑75 (MQ=255) tataAAAGCGCGGCGCATTATAAACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGt > 1:978493/1‑75 (MQ=255) tataAAAGCGCGGCGCATTATAAACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGt > 2:1276240/1‑75 (MQ=255) taAAAGCGCGGCGCATTATAAACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTa > 1:68524/1‑75 (MQ=255) gcggcgCATTATAAACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCa > 1:193482/1‑75 (MQ=255) cggcgCATTATAAACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCaa < 2:1114281/75‑1 (MQ=255) cggcgCATTATAAACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCaa < 2:229222/75‑1 (MQ=255) ggcgCATTATAAACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAAc > 1:540374/1‑75 (MQ=255) gcgcATTATAAACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACg > 1:218031/1‑75 (MQ=255) cgcATTATACACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGa > 1:1104816/1‑75 (MQ=255) cgcATTATAAACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGa < 2:109230/75‑1 (MQ=255) cgcATTATAAACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGa < 1:845831/75‑1 (MQ=255) ataAACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGa < 1:1037935/75‑1 (MQ=255) ataAACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGa < 2:768613/75‑1 (MQ=255) | TCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGA > REL606/2619316‑2619458 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |