Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,625,089	G→T	P156Q (CCG→CAG)	rncS ←	ribonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,625,089	0	G	T	100.0%	138.0 / NA	33	P156Q (CCG→CAG)	rncS	ribonuclease III
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/19);  total (14/19)

CGGACCTGAACTACCAGATAAGTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAGGATTAATTTCTCG  >  REL606/2625017‑2625160                                                                         |                                                                        cGGACCTGAACTACCAGATAAGTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGa                                                                       <  2:798197/75‑1 (MQ=255)       tGAACTACCAGATAAGTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCtttt                                                                 >  2:894493/1‑75 (MQ=255)           cTACCAGATAAGTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGtt                                                             <  1:674970/75‑1 (MQ=255)           cTACCAGATAAGTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGtt                                                             >  2:1582155/1‑75 (MQ=255)            tACCAGATAAGTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGttt                                                            <  1:1086770/75‑1 (MQ=255)                   tAAGTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCt                                                     <  2:1379778/75‑1 (MQ=255)                   tAAGTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCt                                                     >  2:1321849/1‑75 (MQ=255)                     aGTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTgg                                                   <  2:751576/75‑1 (MQ=255)                      gTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTggg                                                  <  1:732009/75‑1 (MQ=255)                       tCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGc                                                 <  1:1216283/75‑1 (MQ=255)                             gcggcagATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAAttt                                           <  1:643102/75‑1 (MQ=255)                               ggcagATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCg                                         >  1:89785/1‑75 (MQ=255)                                  agATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTcc                                      >  2:296517/1‑75 (MQ=255)                                    aTGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAg                                    >  1:1348162/1‑75 (MQ=255)                                       acgacCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACg                                 >  2:101097/1‑75 (MQ=255)                                            ccTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGttt                            <  1:1523515/75‑1 (MQ=255)                                               gCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGAt                         <  1:857176/75‑1 (MQ=255)                                                  aaTATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATAcc                      >  1:458247/1‑75 (MQ=255)                                                  aaTATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATAcc                      >  2:383150/1‑75 (MQ=255)                                                  aaTATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATAcc                      <  2:886928/75‑1 (MQ=255)                                                   atatTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCa                     >  2:1140694/1‑75 (MQ=255)                                                      ttCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGtt                  <  1:1170705/75‑1 (MQ=255)                                                       tCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTg                 <  2:1224040/75‑1 (MQ=255)                                                       tCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTg                 >  2:1592548/1‑75 (MQ=255)                                                          tGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAgg              <  1:750287/75‑1 (MQ=255)                                                              aGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAGGATTa          >  1:239985/1‑75 (MQ=255)                                                                cgcgTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAGGATTAAt        <  1:232938/75‑1 (MQ=255)                                                                 gcgTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAGGACTAAtt       <  2:342752/75‑1 (MQ=255)                                                                  cgTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAGGATTAAttt      <  1:524987/75‑1 (MQ=255)                                                                  cgTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAGGATTAAttt      <  1:524983/75‑1 (MQ=255)                                                                     tttCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAGGATTAATTtctc   <  1:1158834/75‑1 (MQ=255)                                                                     tttCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAGGATTAATTtctc   >  2:1130985/1‑75 (MQ=255)                                                                      ttCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAGGATTAATTTCTCg  >  2:907588/1‑75 (MQ=255)                                                                         |                                                                        CGGACCTGAACTACCAGATAAGTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAGGATTAATTTCTCG  >  REL606/2625017‑2625160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑