Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	REL606	474,391	(GTCGCCAGC)1→2	coding (915/1677 nt)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	474375 =	2 (0.030)	52 (0.990)	37/134	0.4	96.5%	coding (931/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
	?	REL606	= 474383	2 (0.040)					coding (923/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

TGTTTTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/474455‑474375 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gctggcgaCGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCAACGTA  <  REL606/474383‑474309                                                                                                                                                              TGTTTTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCT                                                                                  >  1:1039874/1‑76  GTTTTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCATCAACCGCTGGCAGTGCTGGCGACGCTG                                                                                 <  2:26150/76‑1    TTTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGC                                                                               >  1:1613101/1‑76     TTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCG                                                                              <  1:25375/76‑1         GTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGC                                                                          >  2:955508/1‑76          TCTCCGTCGGGATTTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCT                                                                         <  1:177689/76‑1          TCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCT                                                                         <  1:627162/76‑1           CTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTG                                                                        <  2:361425/76‑1            TCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCGGG                                                                       >  1:555578/1‑76             CCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGC                                                                      >  1:1424211/1‑76              CGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCG                                                                     <  2:1535691/76‑1              CGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCG                                                                     >  2:1366233/1‑76              CGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCG                                                                     <  2:925168/76‑1              CGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCG                                                                     <  1:612843/76‑1               GTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGA                                                                    >  2:1623784/1‑76                  GGGCTGTTGTTTTATCCGTTCATTCTGATCCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTA                                                                 <  2:1382204/76‑1                  GGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTA                                                                 >  2:1637563/1‑76                  GGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTA                                                                 <  2:806910/76‑1                  GGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTA                                                                 >  1:935894/1‑76                  GGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTCGCGGTTA                                                                 >  2:479401/1‑76                   GGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTAT                                                                <  2:1027683/76‑1                    GATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATT                                                               >  2:901804/1‑76                      aGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCT                                                             <  2:386567/75‑1                          GTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTT                                                         <  2:242348/76‑1                               ATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAA                                                    >  2:1785779/1‑76                               ATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAA                                                    >  2:1592482/1‑76                               ATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAA                                                    <  2:1507998/76‑1                                  CGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTC                                                 <  1:1141670/76‑1                                     TAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTT                                              <  2:610386/76‑1                                       ATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAG                                            <  2:1092603/76‑1                                       ATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAG                                            <  2:867858/76‑1                                       ATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAG                                            >  1:1472745/1‑76                                           TGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGC                                        >  1:1463493/1‑76                                            GATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCA                                       >  1:623224/1‑76                                            GATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCA                                       <  1:1368611/76‑1                                             ATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTTTTCTGTTTGGTAAGTCGTTAGCCGCct                                      >  1:1015223/1‑74                                              TTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATT                                     <  1:1425572/76‑1                                              TTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATT                                     <  2:1511361/76‑1                                              TTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATT                                     >  1:412627/1‑76                                              TTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATT                                     <  2:620706/76‑1                                                tAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTT                                   >  2:466432/2‑76                                                CAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGCTTTTTCTGTTTGGTGAGTCGGTGGCCGCGTTTT                                   >  2:259355/1‑76                                                CAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTT                                   >  2:490025/1‑76                                                 AGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTT                                  <  1:992091/76‑1                                                   CAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGCATATTCTGGTTGGGAAGGCGTTAGCCGCATTTTTCC                                >  1:1242447/1‑76                                                        gcgGGCAGTGCTGGCGGCGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTG                           <  1:335052/73‑1                                                          TGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCG                         <  1:123501/76‑1                                                            GCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGAC                       <  2:1660564/76‑1                                                              AGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTG                     >  2:163387/1‑76                                                              AGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTG                     >  2:1251663/1‑76                                                               GTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACCGT                    >  2:1002461/1‑76                                                                TGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTT                   >  2:1199835/1‑76                                                                 GCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTT                  >  2:1084379/1‑76                                                                  CTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTG                 >  1:12731/1‑76                                                                  CTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTG                 >  2:989856/1‑76                                                                   TGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGAATGTTTGG                >  2:1113575/1‑76                                                                          GCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCC         >  1:1434265/1‑76                                                                           CTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCC        >  2:1729862/1‑76                                                                             GGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCAA      <  2:1335317/76‑1                                                                               CGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCAACG    <  1:1093981/76‑1                                                                                GACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCAACGT   <  1:1149096/76‑1                                                                                 ACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCAACGTA  >  2:627627/1‑76                                                                                 ACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCAACGTA  <  1:355471/76‑1                                                                                                                                                              TGTTTTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/474455‑474375 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gctggcgaCGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCAACGTA  <  REL606/474383‑474309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.