| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 124,866 | A→G | intergenic (‑511/‑30) | aroP ← / → pdhR | aromatic amino acid transporter/transcriptional regulator of pyruvate dehydrogenase complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 124,866 | 0 | A | G | 98.5% | 271.3 / ‑5.5 | 67 | intergenic (‑511/‑30) | aroP/pdhR | aromatic amino acid transporter/transcriptional regulator of pyruvate dehydrogenase complex |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (37/29); total (37/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
AAAACCTGTATGGACATAAGGTGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGACTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGT > REL606/124793‑124936 | aaaaCCTGTATGGACATAAGGTGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCt > 1:1446096/1‑76 (MQ=255)aaaaCCTGTATGGACATAAGGTGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCt > 1:354076/1‑76 (MQ=255) aaCCTGTATGGACATAAGGTGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTc > 1:670715/1‑76 (MQ=255) ccTGTATGGACATAAGGTGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCgg < 1:1644377/76‑1 (MQ=255) tATGGACATAAGGTGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAg > 1:824119/1‑76 (MQ=255) aTGGACATAAGGTGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGt > 2:1232085/1‑76 (MQ=255) tGGACATAAGGTGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTg > 1:1543615/1‑76 (MQ=255) gACATAAGGTGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGc < 1:88867/76‑1 (MQ=255) cATAAGGTGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCtt > 1:1003551/1‑76 (MQ=255) aTAAGGTGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTa < 2:1084234/76‑1 (MQ=255) aTAAGGTGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTa > 2:911834/1‑76 (MQ=255) aaGGTGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAg > 1:215528/1‑76 (MQ=255) aaGGTGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAg > 1:270039/1‑76 (MQ=255) aaGGTGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAg > 1:800674/1‑76 (MQ=255) aaGGTGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTAAAg > 2:1018468/1‑76 (MQ=255) ggTGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGAc < 1:872493/76‑1 (MQ=255) tGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAg < 1:1800344/76‑1 (MQ=255) tGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAg < 1:460429/76‑1 (MQ=255) aaTACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGa > 1:884278/1‑76 (MQ=255) aTACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGaa > 2:353744/1‑76 (MQ=255) tACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAAc > 1:291865/1‑76 (MQ=255) aCTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACt < 1:441102/76‑1 (MQ=255) aCTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACt > 2:1377264/1‑76 (MQ=255) aCTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACt < 2:535195/76‑1 (MQ=255) aCTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACt < 2:726497/76‑1 (MQ=255) cTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTc > 1:1520529/1‑76 (MQ=255) tttGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCa > 1:1754836/1‑76 (MQ=255) ttGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCAt > 2:288047/1‑76 (MQ=255) ttGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCAt > 2:313995/1‑76 (MQ=255) ttGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCAt > 2:328544/1‑76 (MQ=255) ttGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCAt > 1:798931/1‑76 (MQ=255) ttGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCAt < 1:901842/76‑1 (MQ=255) tGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATg > 1:889882/1‑76 (MQ=255) gTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATgg < 1:1497042/76‑1 (MQ=255) ttACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGc > 2:248279/1‑76 (MQ=255) ttACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGc > 2:733889/1‑76 (MQ=255) aCTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCt > 2:941621/1‑76 (MQ=255) cTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTa > 2:75457/1‑76 (MQ=255) cTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTa < 1:677760/76‑1 (MQ=255) cTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTa < 1:549142/76‑1 (MQ=255) tttAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTAc < 1:933482/76‑1 (MQ=255) ttAGCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACa < 2:1828588/76‑1 (MQ=255) gCGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCa < 1:1613255/76‑1 (MQ=255) cGTCACAGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCaa < 1:1071653/76‑1 (MQ=255) cacaGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAAt > 2:890392/1‑76 (MQ=255) cacaGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAAt > 1:1801218/1‑76 (MQ=255) acaGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATc > 2:1865803/1‑76 (MQ=255) acaGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATc > 2:744782/1‑76 (MQ=255) caGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATcc > 2:1246235/1‑76 (MQ=255) aGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCg > 2:1655983/1‑76 (MQ=255) aGACATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCg < 2:1816802/76‑1 (MQ=255) aCATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGcc < 2:659571/76‑1 (MQ=255) cATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGcca < 1:865661/76‑1 (MQ=255) cATGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGcca < 1:668279/76‑1 (MQ=255) cATGAAAGTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGcca < 1:668258/76‑1 (MQ=255) aTGAAATTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGccaa > 1:36725/1‑76 (MQ=255) aaaTTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGccaacca < 2:1790747/76‑1 (MQ=255) aaTTGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGccaaccaa > 1:1577466/1‑76 (MQ=255) ttGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCaaaa < 1:818895/76‑1 (MQ=255) tGGTAAGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAAc > 2:1413336/1‑76 (MQ=255) tGGTAAGACCAATTGACTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAAc < 1:963246/76‑1 (MQ=255) aaGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACtctc < 1:512012/76‑1 (MQ=255) aaGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACtctc > 1:723619/1‑76 (MQ=255) aGACCAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTcc < 2:1110576/76‑1 (MQ=255) ccAATTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGAt < 1:287317/76‑1 (MQ=255) aaTTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGAtgt < 2:1487856/76‑1 (MQ=255) aaTTGGCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGAtgt < 2:1632077/76‑1 (MQ=255) | AAAACCTGTATGGACATAAGGTGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGACTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGT > REL606/124793‑124936 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |