| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,145,987 | G→A | E42K (GAA→AAA) | rpoD → | RNA polymerase sigma factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,145,987 | 0 | G | A | 97.0% | 256.1 / ‑1.1 | 66 | E42K (GAA→AAA) | rpoD | RNA polymerase sigma factor |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (33/31); total (33/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
AAGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGA > REL606/3145912‑3146059 | aaGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCg > 2:949734/1‑76 (MQ=255) gAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAg < 2:875139/76‑1 (MQ=255) cAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGAca < 1:480944/76‑1 (MQ=255) aaGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGAcat > 1:905227/1‑76 (MQ=255) aGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGAcatc < 1:1500112/76‑1 (MQ=255) gCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGAcatcat < 1:1504057/76‑1 (MQ=255) gCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGAcatcat < 1:1607997/76‑1 (MQ=255) cTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACatcatc > 2:1023120/1‑76 (MQ=255) cTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACatcatc > 2:1327991/1‑76 (MQ=255) tATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATcc > 1:859833/1‑76 (MQ=255) tATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATcc > 2:568789/1‑76 (MQ=255) aTCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCa > 1:162997/1‑76 (MQ=255) aTCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCa > 1:1441197/1‑76 (MQ=255) cTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCaaa < 2:1102337/76‑1 (MQ=255) tGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAAt > 2:528636/1‑76 (MQ=255) aCCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGa < 1:667919/76‑1 (MQ=255) aCCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGa > 2:1109925/1‑76 (MQ=255) cTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATc < 1:225213/76‑1 (MQ=255) tATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCa < 2:1243280/76‑1 (MQ=255) tATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCa < 1:377150/76‑1 (MQ=255) tATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCa > 2:1408508/1‑76 (MQ=255) tATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCa < 2:1763183/76‑1 (MQ=255) aTGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCaa < 1:485645/76‑1 (MQ=255) aTGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCaa > 2:222424/1‑76 (MQ=255) ccGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGa < 2:411745/76‑1 (MQ=255) cGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGAc < 1:138653/76‑1 (MQ=255) gAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACa < 2:1209997/76‑1 (MQ=255) aGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACAt > 1:1349235/1‑76 (MQ=255) ggTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATg < 2:485143/76‑1 (MQ=255) gTCAATGACCATCTGCCGGAAGATATCGTCGACTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATgg > 1:97023/1‑76 (MQ=255) tCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATggg > 2:1435927/1‑76 (MQ=255) tCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATggg > 2:892090/1‑76 (MQ=255) aTGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCAt < 2:683663/76‑1 (MQ=255) gACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCATTc > 1:1454429/1‑76 (MQ=255) ccATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAg > 2:542054/1‑76 (MQ=255) cATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAgg < 2:1084948/76‑1 (MQ=255) cATCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAgg > 2:775252/1‑76 (MQ=255) aTCTGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGt > 1:813621/1‑76 (MQ=255) tGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGAt < 2:551257/76‑1 (MQ=255) tGCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGAt < 2:578319/76‑1 (MQ=255) gCCGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATg < 1:1162065/76‑1 (MQ=255) ccGGAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATgg > 1:1694323/1‑76 (MQ=255) ggAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGgaa > 1:1725646/1‑76 (MQ=255) ggAAGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGgaa < 1:1708416/76‑1 (MQ=255) aaGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGgaaga > 2:636010/1‑76 (MQ=255) aaGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGgaaga > 1:401797/1‑76 (MQ=255) aGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGgaagaa < 2:885058/76‑1 (MQ=255) aGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGgaagaa < 1:383887/76‑1 (MQ=255) aGATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGAGGgaagaa < 2:715163/76‑1 (MQ=255) gATATCGTCGATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGaagaag > 1:1278150/1‑76 (MQ=255) tcgATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGa < 1:1604122/76‑1 (MQ=255) cgATTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGAt > 1:1806723/1‑76 (MQ=255) aTTCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGc > 1:533501/1‑76 (MQ=255) ttCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGcc > 2:111327/1‑76 (MQ=255) ttCAGATCAGATCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGcc > 1:494349/1‑76 (MQ=255) tcagatcagaTCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCg > 2:1856214/1‑76 (MQ=255) tcagatcagaTCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCg < 2:607320/76‑1 (MQ=255) cagatcagatCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCga > 1:728716/1‑76 (MQ=255) cagatcagatCAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCga > 2:930721/1‑76 (MQ=255) agatcagatcAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCgat > 1:42449/1‑76 (MQ=255) atcagatcAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCgatga < 2:1686072/76‑1 (MQ=255) gatcAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTg < 2:1124508/76‑1 (MQ=255) gatcAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTg < 2:884067/76‑1 (MQ=255) gatcAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTg < 1:717130/76‑1 (MQ=255) atcAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGa < 2:875363/76‑1 (MQ=255) atcAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGa < 1:995667/76‑1 (MQ=255) atcAAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGa > 1:1584565/1‑76 (MQ=255) | AAGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGA > REL606/3145912‑3146059 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |