| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,279,263 | G→A | G29R (GGG→AGG) | yhbG → | predicted transporter subunit: ATP‑binding component of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,279,263 | 0 | G | A | 96.3% | 198.4 / ‑2.1 | 54 | G29R (GGG→AGG) | yhbG | predicted transporter subunit: ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (26/26); total (27/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
ACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCGGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTG > REL606/3279191‑3279335 | aCTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCaggg < 2:329671/76‑1 (MQ=255) cTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGa > 1:527285/1‑76 (MQ=255) cAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAAt < 1:508424/76‑1 (MQ=255) aaaGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAAtc > 2:84394/1‑76 (MQ=255) aaGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAAtcg > 1:129351/1‑76 (MQ=255) aaGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAAtcg > 2:1406017/1‑76 (MQ=255) aaGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAAtcg > 2:1353634/1‑76 (MQ=255) gAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCGGGGAAAtcgtc < 1:1339222/76‑1 (MQ=255) gAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAAtcgtc > 1:1608381/1‑76 (MQ=255) aaCCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAAtcgtcg > 1:873144/1‑76 (MQ=255) aCCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCgg < 1:1516884/76‑1 (MQ=255) ccTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGt > 1:1444663/1‑76 (MQ=255) ttGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTct < 1:1795622/76‑1 (MQ=255) tGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTctg < 2:194957/76‑1 (MQ=255) gCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTctgc > 2:732130/1‑76 (MQ=255) aaaaGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTctgctg > 1:1219352/1‑76 (MQ=255) aaGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTggg < 2:543586/76‑1 (MQ=255) aGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTgggg > 1:352807/1‑76 (MQ=255) ccTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGcc > 2:951713/1‑76 (MQ=255) ataAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCaaa > 1:309095/1‑76 (MQ=255) aaaGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACg < 2:118352/76‑1 (MQ=255) aaGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACgg > 2:404940/1‑76 (MQ=255) aaGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACgg > 2:1542700/1‑76 (MQ=255) aaGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACgg > 2:1510191/1‑76 (MQ=255) aaGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACgg > 1:590736/1‑76 (MQ=255) aaGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACgg > 2:1002953/1‑76 (MQ=255) tCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGt < 1:1028032/76‑1 (MQ=255) tCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGt < 2:1138650/76‑1 (MQ=255) tCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGt < 1:1811610/76‑1 (MQ=255) gcgTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTaa < 1:1401017/76‑1 (MQ=255) cgTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAg < 1:162147/76‑1 (MQ=255) cgTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAg > 2:138050/1‑76 (MQ=255) gTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGTCGGTAAGa < 1:1338779/76‑1 (MQ=255) gTGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGa < 1:152949/76‑1 (MQ=255) tGGTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGac < 1:1308184/76‑1 (MQ=255) gTAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGacca > 2:1842463/1‑76 (MQ=255) tAGAAGACGTCAGCCTGACCGTCAACTCCGGGGAAATCGTCGGTCTGTTGGGGCCAAACGGTGCCGGTAAGaccac > 1:1776491/1‑76 (MQ=255) tAGAAGACGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGATGCCGGTAAGaccac < 1:1412532/76‑1 (MQ=255) agaagaCGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGaccacc > 1:1513349/1‑76 (MQ=255) aagaCGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACcaccac > 2:1264481/1‑76 (MQ=255) aagaCGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACcaccac > 2:1214777/1‑76 (MQ=255) gaCGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACtt > 1:437162/1‑76 (MQ=255) gaCGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACtt > 1:401496/1‑76 (MQ=255) gaCGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACtt < 1:368167/76‑1 (MQ=255) aCGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACttt < 2:810207/76‑1 (MQ=255) cGTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTc < 2:1440654/76‑1 (MQ=255) gTCAGCCTGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCt < 1:951713/76‑1 (MQ=255) tGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGt < 1:1115850/76‑1 (MQ=255) tGACCGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGt > 2:1460441/1‑76 (MQ=255) ccGTCAACTCCAGGAAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGt < 1:93429/76‑1 (MQ=255) cGTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTa > 1:1267696/1‑76 (MQ=255) gTCAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAg < 2:691058/76‑1 (MQ=255) cAACTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGc < 2:1771357/76‑1 (MQ=255) cTCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCAtt < 2:869788/76‑1 (MQ=255) tCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATtg < 1:604390/76‑1 (MQ=255) tCCAGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATtg < 1:594057/76‑1 (MQ=255) | ACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGACCGTCAACTCCGGGGAAATCGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTG > REL606/3279191‑3279335 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |