Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,163,749	A→C	Q965P (CAG→CCG)	rpoB →	DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,163,749	0	A	C	100.0%	179.5 / NA	43	Q965P (CAG→CCG)	rpoB	DNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (27/16);  total (27/16)

CAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCT  >  REL606/4163681‑4163821                                                                     |                                                                         cAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTc                                                                   >  2:455094/1‑76 (MQ=255) cAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTc                                                                   >  1:391373/1‑76 (MQ=255)  aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCg                                                                  <  1:1475333/76‑1 (MQ=255)  aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCg                                                                  <  1:997734/76‑1 (MQ=255)  aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCg                                                                  >  1:1675662/1‑76 (MQ=255)  aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCg                                                                  >  1:6374/1‑76 (MQ=255)  aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCg                                                                  <  2:20876/76‑1 (MQ=255)   aaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGa                                                                 >  1:715231/1‑76 (MQ=255)    aCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGaa                                                                >  2:882680/1‑76 (MQ=255)    aCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGaa                                                                <  2:1290579/76‑1 (MQ=255)      gTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGc                                                              >  1:306708/1‑76 (MQ=255)      gTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGc                                                              >  2:650845/1‑76 (MQ=255)         cgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCggg                                                           >  1:895306/1‑76 (MQ=255)          gcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGt                                                          <  1:1506022/76‑1 (MQ=255)            tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCt                                                        <  2:768905/76‑1 (MQ=255)              gAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGt                                                      >  2:427062/1‑76 (MQ=255)               aaaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGACCTGCCGATCCTCGAAGCGGGTCTGtt                                                     <  1:614546/76‑1 (MQ=255)                aaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTc                                                    <  2:954226/76‑1 (MQ=255)                       gaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTa                                             >  2:1496450/1‑76 (MQ=255)                               gCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgct                                     >  1:1634720/1‑76 (MQ=255)                                  cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtg                                  >  2:1013660/1‑76 (MQ=255)                                  cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtg                                  >  2:1685515/1‑76 (MQ=255)                                   aaaCAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgc                                 >  2:1283847/1‑76 (MQ=255)                                     aCAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGtgctgtgctg                               <  1:1716465/76‑1 (MQ=255)                                     aCAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGtgctgtgctg                               <  1:427062/76‑1 (MQ=255)                                      cAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTgg                              <  2:1544402/76‑1 (MQ=255)                                       aGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGt                             >  2:1584801/1‑76 (MQ=255)                                         gCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAg                           <  2:1453612/76‑1 (MQ=255)                                           gaagaaAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGcc                         <  1:1762646/76‑1 (MQ=255)                                            aagaaagcCCGGTCTGAAGAACTCCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCg                        <  2:1384174/76‑1 (MQ=255)                                            aagaaagaCCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCg                        >  1:1104836/1‑76 (MQ=255)                                            aagaaagaCCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCg                        >  1:250336/1‑76 (MQ=255)                                            aagaaagaCCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGGTCAGCCGTATCCGTGCTGTGCTGGTAGCCg                        >  1:844498/1‑76 (MQ=255)                                               aaagaCCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTg                     >  2:1210927/1‑76 (MQ=255)                                                aagaCCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg                    >  2:1052710/1‑76 (MQ=255)                                                 agaCCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGc                   <  1:594637/76‑1 (MQ=255)                                                     cTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTg               >  2:95354/1‑76 (MQ=255)                                                       gTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGaa             >  2:1177969/1‑76 (MQ=255)                                                       gTCTGAAAACCGGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGaa             <  2:929443/76‑1 (MQ=255)                                                            aagaaCTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTga        >  2:1611538/1‑76 (MQ=255)                                                            aagaaCTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTga        >  2:462608/1‑76 (MQ=255)                                                              gatcTGCGGATCCGCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgaga      <  1:680733/73‑1 (MQ=255)                                                               atcTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGaa     <  2:755379/74‑1 (MQ=255)                                                               aaCTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGaa     <  2:188643/76‑1 (MQ=255)                                                                aCTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAg    >  1:835620/1‑76 (MQ=255)                                                                  tGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGGAGCTGAGAAGCt  >  1:1476199/1‑76 (MQ=255)                                                                  tGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCt  <  2:1780994/76‑1 (MQ=255)                                                                     |                                                                         CAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCT  >  REL606/4163681‑4163821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑