Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,165,450	C→A	R131L (CGC→CTC)	yehU ←	predicted sensory kinase in two‑component system with YehT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,165,450	0	C	A	100.0%	138.9 / NA	38	R131L (CGC→CTC)	yehU	predicted sensory kinase in two‑component system with YehT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (13/25);  total (13/25)

ATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGCGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGCAACTTAG  >  REL606/2165378‑2165520                                                                         |                                                                       aTTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGAc                                                                     >  2:1494322/1‑76 (MQ=255)  tttGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACg                                                                    >  1:1355546/1‑76 (MQ=255)  tttGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACg                                                                    <  1:1280607/76‑1 (MQ=255)   ttGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACgg                                                                   <  2:836977/76‑1 (MQ=255)    tGCACCATTTCAGCGACGAACGTGACTGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGa                                                                  <  2:174202/76‑1 (MQ=255)     gCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGAt                                                                 <  2:1201793/76‑1 (MQ=255)      cACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATc                                                                <  1:1562837/76‑1 (MQ=255)       aCCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCa                                                               >  1:232091/1‑76 (MQ=255)           tttCAGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGATACGGATCAGGAt                                                           <  2:602305/76‑1 (MQ=255)               aGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCtg                                                       >  2:1406510/1‑76 (MQ=255)                  gacgaACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTgtgt                                                    >  1:754377/1‑76 (MQ=255)                  gacgaACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTgtgt                                                    <  2:1019960/76‑1 (MQ=255)                   acgaACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGGTCAGGATGCTGTGTa                                                   >  2:943656/1‑76 (MQ=255)                          ggACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGgcc                                            <  1:870152/75‑1 (MQ=255)                                 aCCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGc                                     <  2:1724022/76‑1 (MQ=255)                                   cggcggTAATGGAGTTAAAGACTTTATCAGTGCGCTCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCaa                                   >  1:449662/1‑76 (MQ=255)                                     gcggTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATc                                 <  1:4740/76‑1 (MQ=255)                                      cggTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATcc                                <  2:1622981/76‑1 (MQ=255)                                        gTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCtt                              <  2:751492/76‑1 (MQ=255)                                        gTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCtt                              <  1:286496/76‑1 (MQ=255)                                         tAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTc                             <  1:688361/76‑1 (MQ=255)                                          aaTGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCa                            <  1:469894/76‑1 (MQ=255)                                           aTGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCaa                           >  2:1130501/1‑76 (MQ=255)                                            gggAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCTAGCAATCCTTCAAc                          <  1:64829/75‑1 (MQ=255)                                             ggAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACg                         >  2:958923/1‑76 (MQ=255)                                               aGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGAt                       <  1:872782/76‑1 (MQ=255)                                               aGTTAAAGACTTTATCAGCGCGCCAGAGACGGATCAGGATGCTGTGTACCAGGCCGCCCAGCAATCATTCAACGAt                       >  2:717824/1‑76 (MQ=255)                                                gTTAAAGCCTCTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATg                      <  2:496049/76‑1 (MQ=255)                                                gTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATg                      >  2:1668646/1‑76 (MQ=255)                                                 ttAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATgg                     >  2:1542045/1‑76 (MQ=255)                                                   aaaGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGtt                   >  2:176911/1‑76 (MQ=255)                                                   aaaGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGtt                   <  2:834777/76‑1 (MQ=255)                                                        cTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGAt              <  2:1691517/76‑1 (MQ=255)                                                             tCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGc         <  1:904781/76‑1 (MQ=255)                                                              cAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGCa        >  1:1499093/1‑76 (MQ=255)                                                                gTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGCAAc      >  1:801539/1‑76 (MQ=255)                                                                  gcgcCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGCAACtt    <  1:309301/76‑1 (MQ=255)                                                                   cgcCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGCAACTTa   <  2:1645635/76‑1 (MQ=255)                                                                   cgcCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGCAACTTa   <  2:92307/76‑1 (MQ=255)                                                                    ggccGTGACGGATCGGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGCAACTTAg  <  2:338888/74‑1 (MQ=255)                                                                    gcCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGCAACTTAg  <  1:888514/76‑1 (MQ=255)                                                                    gcCCGAGACGGATCAGGATGCTGAGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGCAACTTAg  >  2:67909/1‑76 (MQ=255)                                                                         |                                                                       ATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGCGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGCAACTTAG  >  REL606/2165378‑2165520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑