New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 2040253	59 (0.880)	4 (0.070)	4/136	8.3	8.9%	intergenic (‑125/+18)	vioB/vioA	VioB, involved in dTDP‑N‑acetylviosamine synthesis/VioA, involved in dTDP‑N‑acetylviosamine synthesis
	?	REL606	= 2040442	28 (0.460)					coding (942/1113 nt)	vioA	VioA, involved in dTDP‑N‑acetylviosamine synthesis
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTGGCTATTGGTAGATTATCAACGCTAGCTGAAATCAATCCTCGATACATAGGCATATTACTGATTAATGGATAAAAATACTTTCGAGATAGGATATTATTTTTCTTAAGTAATTCATATGCCTGATCACGACTCATATGAA  >  REL606/2040368‑2040509                                                                           |                                                                    ttggctattggTAGATTATCAACGCTAGCTGAAATCAATCCTCGATACATAGGCATATTACTGATTAATGGATaa                                                                     <  1:532762/75‑1 (MQ=255)  tggctattggTAGATTATCAACGCTAGCTGAAATCAATCCTCGATACATAGGCATATTACTGATTAATGGATaaa                                                                    <  2:76236/75‑1 (MQ=255)   ggctattggTAGATTATCAACGCTAGCTGAAATCAATCCTCGATACATAGGCATATTACTGATTAATGGATaaaa                                                                   >  1:629931/1‑75 (MQ=255)      tattggTCGATTATCAACGCTAGCTGAAATCACTCCTCGATACAAAGGCATATTACTGATTAATGGATAAAAATa                                                                >  2:1115626/1‑75 (MQ=255)      tattggTAGATTATCAACGCTAGCTGAAATCAATCCTCGATACATAGGCATATTACTGATTAATGGATAAAAATa                                                                >  2:1115598/1‑75 (MQ=255)      tattggTAGATTATCAACGCTAGCTGAAATCAATCCTCGATACATAGGCATATTACTGATTAATGGATAAAAATa                                                                >  2:648970/1‑75 (MQ=255)        ttggTAGATTATCAACGCTAGCTGAAATCAATCCTCGATACATAGGCATATTACTGATTAATGGATAAAAATACt                                                              <  1:49578/75‑1 (MQ=255)           gTAGATTATCAACGCTAGCTGAAATCAATCCTCGATACATAGGCATATTACTGATTAATGGATAAAAATACTTTc                                                           <  1:989231/75‑1 (MQ=255)             aGATTATCAACGCTAGCTGAAATCAATCCTCGATACATAGGCATATTACTGATTAATGGATAAAAATACTTTCga                                                         <  1:1492950/75‑1 (MQ=255)             aGATTATCAACGCTAGCTGAAATCAATCCTCGATACATAGGCATATTACTGATTAATGGATAAAAATACTTTCga                                                         <  1:1535102/75‑1 (MQ=255)             aGATTATCAACGCTAGCTGAAATCAATCCTCGATACATAGGCATATTACTGATTAATGGATAAAAATACTTTCga                                                         <  2:64305/75‑1 (MQ=255)              gATTATCAACGCTAGCTGAAATCAATCCTCGATACATAGGCATATTACTGATTAATGGATAAAAATACTTTCgag                                                        >  1:1085697/1‑75 (MQ=255)                ttATCAACGCTAGCTGAAATCAATCCTCGATACATAGGCATATTACTGATTAATGGATAAAAATACTTTCGAGAt                                                      <  1:1789177/75‑1 (MQ=255)                        gCTAGCTGAAATCAATCCTCGATACATAGGCATATTACTGATTAATGGATAAAAATACTTTCGAGATAGGAtatt                                              <  1:343490/75‑1 (MQ=255)                         cTAGCTGAAATCAATCCTCGATACATAGGCATATTACTGATTAATGGATAAAAATACTTTCGAGATAGGAtatta                                             <  2:543045/75‑1 (MQ=255)                         cTAGCTGAAATCAATCCTCGATACATAGGCATATTACTGATTAATGGATAAAAATACTTTCGAGATAGGAtatta                                             <  2:1307730/75‑1 (MQ=255)                           aGCTGAAATCAATCCTCGATACATAGGCATATTACTGATTAATGGATAAAAATACTTTCGAGATAGGATattatt                                           >  2:435725/1‑75 (MQ=255)                            gCTGAAATCAATCCTCGATACATAGGCATATTACTGATTAATGGATAAAAATACTTTCGAGATAGGATATTAttt                                          >  2:1949372/1‑75 (MQ=255)                             cTGAAATCAATCCTCGATACATAGGCATATTACTGATTAATGGATAAAAATACTTTCGAGATAGGATATTAtttt                                         <  1:1137423/75‑1 (MQ=255)                                  atcaatcCTCGATACATAGGCATATTACTGATTAATGGATAAAAATACTTTCGAGATAGGATATTATTTTTCTTa                                    <  1:947735/75‑1 (MQ=255)                                  atcaatcCTCGATACATAGGCATATTACTGATTAATGGATAAAAATACTTTCGAGATAGGATATTATTTTTCTTa                                    <  1:1304339/75‑1 (MQ=255)                                   tcaatcCTCGATACATAGGCATATTACTGATTAATGGATAAAAATACTTTCGAGATAGGATATTATTTTTCTTaa                                   >  1:52395/1‑75 (MQ=255)                                     aatcCTCGATACATAGGCATATTACTGATTAATGGATAAAAATACTTTCGAGATAGGATATTATTTTTCTTAAGt                                 <  2:482968/75‑1 (MQ=255)                                            gATACATAGGCATATTACTGATTAATGGATAAAAATACTTTCGAGATAGGATATTATTTTTCTTAAGTAATTCat                          <  1:448673/75‑1 (MQ=255)                                               aCATAGGCATATTACTGATTAATGGATAAAAATACTTTCGAGATAGGATATTATTTTTCTTAAGTAATTCATATg                       >  2:1646273/1‑75 (MQ=255)                                                 aTAGGCATATTACTGATTAATGGATAAAAATACTTTCGAGATAGGATATTATTTTTCTTAAGTAATTCATATGcc                     >  1:621890/1‑75 (MQ=255)                                                 aTAGGCATATTACTGATTAATGGATAAAAATACTTTCGAGATAGGATATTATTTTTCTTAAGTAATTCATATGcc                     >  2:767658/1‑75 (MQ=255)                                                   aGGCATATTACTGATTAATGGATAAAAATACTTTCGAGATAGGATATTATTTTTCTTAAGTAATTCATATGCCTg                   <  1:881008/75‑1 (MQ=255)                                                     gCATATTACTGATTAATGGATAAAAATACTTTCGAGATAGGATATTATTTTTCTTAAGTAATTCATATGCCTGAt                 <  2:90638/75‑1 (MQ=255)                                                         atTACTGATTAATGGATAAAAATACTTTCGAGATAGGATATTATTTTTCTTAAGTAATTCATATGCCTGATCACg             >  2:397145/1‑75 (MQ=255)                                                          ttACTGATTAATGGATAAAAATACTTTCGAGATAGGATATTATTTTTCTTAAGTAATTCATATGCCTGATCACGa            >  2:145124/1‑75 (MQ=255)                                                               gATTAATGGATAAAAATACTTTCGAGATAGGATATTATTTTTCTTAAGTAATTCATATGCCTGATCACGACTCat       >  2:1827338/1‑75 (MQ=255)                                                                 ttAATGGATAAAAATACTTTCGAGATAGGATATTATTTTTCTTAAGTAATTCATATGCCTGATCACGACTCatat     >  1:1882332/1‑75 (MQ=255)                                                                    aTGGATAAAAATACTTTCGAGATAGGATATTATTTTTCTTAAGTAATTCATATGCCTGATCACGACTCATATGaa  >  1:292412/1‑75 (MQ=255)                                                                           |                                                                    TTGGCTATTGGTAGATTATCAACGCTAGCTGAAATCAATCCTCGATACATAGGCATATTACTGATTAATGGATAAAAATACTTTCGAGATAGGATATTATTTTTCTTAAGTAATTCATATGCCTGATCACGACTCATATGAA  >  REL606/2040368‑2040509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.