| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,464,461 | T→G | Y31S (TAT→TCT) | envZ ← | osmolarity sensor protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,464,461 | 0 | T | G | 98.5% | 258.1 / NA | 66 | Y31S (TAT→TCT) | envZ | osmolarity sensor protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); major base G (27/37); minor base C (0/1); total (27/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGA > REL606/3464389‑3464534 | cGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGaa < 1:742478/75‑1 (MQ=255) gCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAg < 2:1345413/75‑1 (MQ=255) cACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAgt > 1:428329/1‑75 (MQ=255) aCTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAgtc > 1:1380891/1‑75 (MQ=255) gTACGCGAGGACTTTATTAAACTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCAc < 1:1236668/75‑1 (MQ=255) tACGCGAGGACTTTATTAAACTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTGGGCAcc > 1:860707/1‑75 (MQ=255) tACGCGAGGACTTTATTAAACTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCAcc > 2:1294138/1‑75 (MQ=255) tACGCGAGGACTTTATTAAACTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCAcc > 1:2114262/1‑75 (MQ=255) aCGCGAGGACTTTATTAAACTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCa > 1:1716855/1‑75 (MQ=255) cgAGGACTTTATTAAACTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGc < 1:1440943/75‑1 (MQ=255) gAGGACTTTATTAAACTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCt > 1:1426845/1‑75 (MQ=255) gAGGACTTTATTAAACTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCt < 1:1944956/75‑1 (MQ=255) tttATTAAACTGCTGGCGGCTCAGCAAACTCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCggc > 1:1166013/1‑73 (MQ=255) tttATTAAACTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGaa > 1:1166009/1‑75 (MQ=255) tttATTAAACTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGaa > 2:1850491/1‑75 (MQ=255) ttattaAACTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAAc < 2:1651309/75‑1 (MQ=255) tattaAACTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACa < 1:1826102/75‑1 (MQ=255) attaAACTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGACGTCGCCACCAGGCTGGCGAACAg > 2:1563241/1‑75 (MQ=255) attaAACTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAg < 2:1102034/75‑1 (MQ=255) ttaAACTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCAACATGATGGCGAAAAGc > 1:443531/1‑75 (MQ=255) aaaCTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCaa < 1:206630/75‑1 (MQ=255) aCTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAgg < 2:1590132/75‑1 (MQ=255) tgctgGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCCGGCTGGGGAACAGCCAgggg > 1:584792/1‑73 (MQ=255) tgctgGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAgggg > 2:209906/1‑73 (MQ=255) tgctgGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTg < 2:1953865/75‑1 (MQ=255) gctgGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGa < 1:1869823/75‑1 (MQ=255) tgGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACg > 2:1834328/1‑75 (MQ=255) tgGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAAAAGCAAGGGGACg > 2:862988/1‑75 (MQ=255) tgGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCACGCTGGGGAAAAACAAGGTGACg > 2:1701718/1‑75 (MQ=255) ggAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGcgacga > 2:1523134/1‑75 (MQ=255) ggAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGa < 2:1348556/75‑1 (MQ=255) gAGGCTCAGCAAAATCGCGAGGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGAt < 2:1837770/75‑1 (MQ=255) gAGGCTAAGCAACATCGCGGCCCTCCGCAACCCGAGAGCAGTCGTCACCCGGCGGTCGAACGGCAAGGTGACGAt < 2:565741/75‑1 (MQ=255) aGGCTCAGCAAAATCGCGAAGTTCAGCACCACCGGAGAAGTCGTCTCCAGGCTGGCGAACAGCAAGGTGACGATg < 2:1967288/75‑1 (MQ=255) aGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATg < 2:1206198/75‑1 (MQ=255) aGGCTCAGCAAAATCGCAAAGTTCAGCACCACCAGACAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATg < 2:839112/75‑1 (MQ=255) ggCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGa < 2:2052304/75‑1 (MQ=255) gCTCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAg < 2:1114987/75‑1 (MQ=255) tCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCCAGGTGACGCTGAGCa > 2:1819069/1‑75 (MQ=255) tCAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCa > 1:2093861/1‑75 (MQ=255) tCAGCAAAAACGCGAAGTTCAGCCCCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCa < 2:1584585/75‑1 (MQ=255) cAGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCCGGCGGAAAGCAAGGGGGCGATGAGCaa > 1:53414/1‑75 (MQ=255) aGCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAAt < 2:814943/75‑1 (MQ=255) gCAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCaaga < 2:95482/75‑3 (MQ=255) cAAAATCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATaa < 2:1802547/75‑1 (MQ=255) aaaTCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATaaag > 1:130425/1‑73 (MQ=255) aaaTCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACg < 2:1866574/75‑1 (MQ=255) aaaTCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACg < 2:709136/75‑1 (MQ=255) aaaTCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACg < 2:1597903/75‑1 (MQ=255) aaaTCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACg < 2:1032128/75‑1 (MQ=255) aaTCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGt < 2:218198/75‑1 (MQ=255) aTCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTa < 1:1910751/75‑1 (MQ=255) aTCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTa < 1:1882570/75‑1 (MQ=255) tCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTAc > 2:1827494/1‑75 (MQ=255) tCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTAc < 2:1771157/75‑1 (MQ=255) tCGCGAAGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTAc < 2:517311/75‑1 (MQ=255) gcgAACTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAAAAGCAAGGTGACGATGAGCAATAACGTACgg < 2:1229504/75‑1 (MQ=255) aaGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCa > 2:552497/1‑75 (MQ=255) aGTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAAAAAGAAGGGGACGGTGAGCAATAACGTACGGGCaa > 2:1730570/1‑75 (MQ=255) gTTCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCaaa < 1:1064665/75‑1 (MQ=255) tccAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAAt < 2:329574/73‑1 (MQ=255) tCAGCACCACCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGAGACGATGAGCAATAACGTACGGGCAAATg > 2:55726/1‑75 (MQ=255) caccacCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACt < 1:2127935/75‑1 (MQ=255) caccacCAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACt < 2:1379177/75‑1 (MQ=255) accaccAGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACtt > 2:242343/1‑75 (MQ=255) accaGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGt < 2:432631/75‑1 (MQ=255) accaGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGt < 2:186476/75‑1 (MQ=255) ccaGAGAAGTCGTCACCAGGCTGGCGAACAGCAAGGGGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTg > 1:1392011/1‑75 (MQ=255) aTAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCga < 2:538948/75‑1 (MQ=255) | CGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCAGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGA > REL606/3464389‑3464534 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |