New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	2040964 =	36 (0.630)	6 (0.110)	4/140	6.9	11.3%	coding (420/1113 nt)	vioA	VioA, involved in dTDP‑N‑acetylviosamine synthesis
	?	REL606	2041449 =	61 (1.140)					coding (1372/1428 nt)	wzx	O antigen translocase (Wzx)
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AATTTACACCGAATGCATGTGCTGCGTCGTAGATTACTTTCAATCCATAATTATCAGCTATTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTAAAATTGCTGAAGTTTTTGGTGTAATAGCCTGTTCTATT  >  REL606/2040891‑2041039                                                                          |                                                                            aaTTTACACCGAATGCATGTGCTGCGTCGTAGATTACTTTCAATCCATAATTATCAGCTATTTTTTGAATttcttc                                                                           <  2:293994/76‑1 (MQ=255)     tACACCGAATGCATGTGCTGCGTCGTAGATTACTTTCAATCCATAATTATCAGCTATTTTTTGAATTTCTTCGACt                                                                       >  2:791303/1‑76 (MQ=255)        acCGAATGCATGTGCTGCGTCGTAGATTACTTTCAATCCATAATTATCAGCTATTTTTTGAATTTCTTCGACTTca                                                                    <  1:810248/76‑1 (MQ=255)         ccGAATGCATGTGCTGCGTCGTAGATTACTTTCAATCCATAATTATCAGCTATTTTTTGAATTTCTTCGACTTcac                                                                   >  2:65463/1‑76 (MQ=255)            aaTGCATGTGCTGCGTCGTAGATTACTTTCAATCCATAATTATCAGCTATTTTTTGAATTTCTTCGACTTCACAgg                                                                >  1:342958/1‑76 (MQ=255)            aaTGCATGTGCTGCGTCGTAGATTACTTTCAATCCATAATTATCAGCTATTTTTTGAATTTCTTCGACTTCACAgg                                                                <  2:1774251/76‑1 (MQ=255)                  tgtgCTGCGTCGTAGATTACTTTCAATCCATAATTATCAGCTATTTTTTGAATTTCTTCGACTTCACAGGGTGTTg                                                          <  2:686396/76‑1 (MQ=255)                  tgtgCTGCGTCGTAGATTACTTTCAATCCATAATTATCAGCTATTTTTTGAATTTCTTCGACTTCACAGGGTGTTg                                                          <  2:61918/76‑1 (MQ=255)                      ctgcGTCGTAGATTACTTTCAATCCATAATTATCAGCTATTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTa                                                      <  2:1619808/76‑1 (MQ=255)                            cgtAGATTACTTTCAATCCATAATTATCAGCTATTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATg                                                >  2:16011/1‑76 (MQ=255)                               aGATTACTTTCAATCCATAATTATCAGCTATTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGAc                                             <  1:1382009/76‑1 (MQ=255)                                gATTACTTTCAATCCATAATTATCAGCTATTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACa                                            >  1:151187/1‑76 (MQ=255)                                gATTACTTTCAATCCATAATTATCAGCTATTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACa                                            >  1:151196/1‑76 (MQ=255)                                   tACTTTCAATCCATAATTATCAGCTATTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACAGGt                                         <  1:441022/76‑1 (MQ=255)                                     cTTTCAATCCATAATTATCAGCTATTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTaa                                       >  1:1729890/1‑76 (MQ=255)                                      tttCAATCCATAATTATCAGCTATTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTaaa                                      <  1:1118315/76‑1 (MQ=255)                                      tttCAATCCATAATTATCAGCTATTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTaaa                                      >  1:347463/1‑76 (MQ=255)                                        tCAATCCATAATTATCAGCTATTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTAAAAt                                    <  2:1008448/76‑1 (MQ=255)                                          aaTCCATAATTATCAGCTATTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTAAAATTg                                  <  2:928181/76‑1 (MQ=255)                                                tAATTATCAGCTATTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTAAAATTGCTGAAg                            >  2:963030/1‑76 (MQ=255)                                                tAATTATCAGCTATTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTAAAATTGCTGAAg                            >  1:707282/1‑76 (MQ=255)                                                tAATTATCAGCTATTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTAAAATTGCTGAAg                            >  1:1350440/1‑76 (MQ=255)                                                 aaTTATCAGCTATTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTAAAATTGCTGAAGt                           >  1:1203493/1‑76 (MQ=255)                                                       cAGCTATTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTAAAATTGCTGAAGTTTTTgg                     >  1:572648/1‑76 (MQ=255)                                                          cTATTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTAAAATTGCTGAAGTTTTTGgtgt                  <  1:1588829/76‑1 (MQ=255)                                                           tATTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTAAAATTGCTGAAGTTTTTGGTGTa                 <  2:726115/76‑1 (MQ=255)                                                           tATTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTAAAATTGCTGAAGTTTTTGGTGTa                 <  1:1494419/76‑1 (MQ=255)                                                            aTTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTATCAATGGACAGGTAAAATTGCTGAAGTTTTTGGTGTaa                <  2:220392/76‑1 (MQ=255)                                                             ttttttGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTAAAATTGCTGAAGTTTTTGGTGTAAt               >  2:1471226/1‑76 (MQ=255)                                                               ttttGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTAAAATTGCTGAAGTTTTTGGTGTAATAg             <  1:1172133/76‑1 (MQ=255)                                                                tttGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTAAAATTGCTGAAGTTTTTGGTGTAATAGc            >  2:262031/1‑76 (MQ=255)                                                                 ttGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTAAAATTGCTGAAGTTTTTGGTGTAATAGcc           <  1:1171847/76‑1 (MQ=255)                                                                   gAATTTCTTCGACTGCACAGGGTGTTGAGTAGCAATGGACAGGTAAAATTGCTGAAGTTTTTGGTGTAATAGCCTg         <  2:1263880/76‑1 (MQ=255)                                                                       ttcttcGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTAAAATTGCTGAAGTTTTTGGTGTAATAGCCTGTTCt     >  1:1695638/1‑76 (MQ=255)                                                                       ttcttcGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTAAAATTGCTGAAGTTTTTGGTGTAATAGCCTGTTCt     >  1:574564/1‑76 (MQ=255)                                                                        tcttcGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTAAAATTGCTGAAGTTTTTGGTGTAATAGCCTGTTCTa    <  2:1392964/76‑1 (MQ=255)                                                                          ttcGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTAAAATTGCTGAAGTTTTTGGTGTAATAGCCTGTTCTAtt  <  1:979071/76‑1 (MQ=255)                                                                          |                                                                            AATTTACACCGAATGCATGTGCTGCGTCGTAGATTACTTTCAATCCATAATTATCAGCTATTTTTTGAATTTCTTCGACTTCACAGGGTGTTGAGTAGCAATGGACAGGTAAAATTGCTGAAGTTTTTGGTGTAATAGCCTGTTCTATT  >  REL606/2040891‑2041039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.