New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	2041277 =	22 (0.380)	8 (0.150)	7/136	5.3	16.2%	coding (107/1113 nt)	vioA	VioA, involved in dTDP‑N‑acetylviosamine synthesis
	?	REL606	2041470 =	63 (1.220)					coding (1351/1428 nt)	wzx	O antigen translocase (Wzx)
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTTTTTTATAAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCATTTATAATATAGTT  >  REL606/2041396‑2041536                                                                           |                                                                   tttttttATAAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCa                                                                   <  1:740785/76‑1 (MQ=255) tttttttATAAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCa                                                                   <  1:740775/76‑1 (MQ=255)   tttttATAAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCAtt                                                                 <  1:1434195/76‑1 (MQ=255)    ttttATAAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCAttt                                                                <  1:1286783/76‑1 (MQ=255)     tttATAAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGTTGTATATAATGGCATTta                                                               <  1:943985/76‑1 (MQ=255)      ttATAAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATAGCATTtaa                                                              <  1:1776901/76‑1 (MQ=255)       tataAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTtaat                                                             >  1:653900/1‑76 (MQ=255)       tataAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTtaat                                                             <  1:201538/76‑1 (MQ=255)       tataAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTtaat                                                             >  1:653890/1‑76 (MQ=255)        ataAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTtaata                                                            <  1:1208741/76‑1 (MQ=255)        ataAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTtaata                                                            >  1:1769547/1‑76 (MQ=255)         taAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTtaataa                                                           <  1:695390/76‑1 (MQ=255)           agagAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATaaaa                                                         <  1:1464879/76‑1 (MQ=255)             agaAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATa                                                       <  1:1340644/76‑1 (MQ=255)             agaAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATa                                                       <  1:1340624/76‑1 (MQ=255)             agaAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATa                                                       <  1:1340614/76‑1 (MQ=255)             agaAATAATAGTCATAAATATTTCTTTGTACATAATTCAAATTAGTGTGATGTATATAATGGCATTTAATAAAATa                                                       <  1:542247/76‑1 (MQ=255)                aataatAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTa                                                    <  1:39943/76‑1 (MQ=255)                aataatAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTa                                                    >  2:247095/1‑76 (MQ=255)                 ataataGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTaa                                                   <  1:590185/76‑1 (MQ=255)                 ataataGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTaa                                                   >  2:1513994/1‑76 (MQ=255)                 ataataGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTaa                                                   >  2:1513999/1‑76 (MQ=255)                   aataGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAat                                                 <  1:1299334/76‑1 (MQ=255)                   aataGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAat                                                 <  1:1299314/76‑1 (MQ=255)                    ataGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAata                                                <  1:987316/76‑1 (MQ=255)                    ataGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAata                                                <  1:485865/76‑1 (MQ=255)                    ataGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAata                                                <  1:1126910/76‑1 (MQ=255)                     taGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAACGGCATTTAATAAAATAGTAAatat                                               <  1:190081/76‑1 (MQ=255)                      aGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAatatt                                              <  1:10290/76‑1 (MQ=255)                      aGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAatatt                                              <  1:250318/76‑1 (MQ=255)                       gTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAatatta                                             <  1:632647/76‑1 (MQ=255)                         cATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAatattata                                           <  1:518680/76‑1 (MQ=255)                         cATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAatattata                                           <  1:518698/76‑1 (MQ=255)                         cATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAatattata                                           <  1:624116/76‑1 (MQ=255)                         cATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAatattata                                           <  1:624130/76‑1 (MQ=255)                          aTAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAatattatat                                          <  2:1398896/76‑1 (MQ=255)                             aaTATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTta                                       <  1:209848/76‑1 (MQ=255)                              atatTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTtaa                                      <  1:895317/76‑1 (MQ=255)                              atatTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTtaa                                      <  1:1797186/76‑1 (MQ=255)                                   tCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAg                                 <  1:1334681/76‑1 (MQ=255)                                    cTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAgg                                >  2:5661/1‑76 (MQ=255)                                    cTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAgg                                <  1:1149468/76‑1 (MQ=255)                                      ttGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGtt                              <  1:393558/76‑1 (MQ=255)                                         tACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCa                           <  1:26041/76‑1 (MQ=255)                                           cATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATa                         >  2:1169831/1‑76 (MQ=255)                                             tAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACt                       <  1:1464676/76‑1 (MQ=255)                                             tAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACt                       >  2:1238824/1‑76 (MQ=255)                                              aaTTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACtt                      <  1:1428858/76‑1 (MQ=255)                                                  cGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTAttt                  <  1:1237699/76‑1 (MQ=255)                                                  cGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTAttt                  <  2:1237699/76‑1 (MQ=255)                                                     aTTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCAt               <  1:684342/76‑1 (MQ=255)                                                     aTTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCAt               >  2:10883/1‑76 (MQ=255)                                                             gATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCATTTataata       <  2:1085207/76‑1 (MQ=255)                                                             gATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCATTTataata       <  2:1085213/76‑1 (MQ=255)                                                             gATGTATATAAGGGCATTAAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCATTTataata       <  1:1085207/76‑1 (MQ=255)                                                             gATGTATAAAATGGCATTTAATAAAATAGTAAATATTATGTTTAATAAAGGTTCCATACTTATTTCATTTataata       <  1:1085213/76‑1 (MQ=255)                                                               tGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCATTTATAAtata     <  1:480385/76‑1 (MQ=255)                                                               tGTATATAATGGCATTTAATAAAATAGGAAATATTATATTTAATAAAGGTTCCATACTTATTTCATTTATAAtata     <  2:480385/76‑1 (MQ=255)                                                               tGTATATAATAGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCATTTATAAtata     <  1:480373/76‑1 (MQ=255)                                                               ggTATTTAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCATTTATAAtata     <  2:480373/75‑1 (MQ=255)                                                                gTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCATTTATAATATAg    <  2:53425/76‑1 (MQ=255)                                                                  atataATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCATTTATAATATAGtt  <  1:202436/76‑1 (MQ=255)                                                                  atataATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCATTTATAATATAGtt  <  2:202436/76‑1 (MQ=255)                                                                           |                                                                   TTTTTTTATAAGAGAAATAATAGTCATAAATATTTCTTTGTACATAATTCGAATTAGTGTGATGTATATAATGGCATTTAATAAAATAGTAAATATTATATTTAATAAAGGTTCCATACTTATTTCATTTATAATATAGTT  >  REL606/2041396‑2041536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.