| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 99,763 | C→A | R320S (CGC→AGC) | mraY → | phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 99,763 | 0 | C | A | 98.3% | 220.5 / ‑3.3 | 59 | R320S (CGC→AGC) | mraY | phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (30/28); minor base G (1/0); total (31/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01 | |||||||||||
CGTGTTCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGTTTCTGG > REL606/99690‑99834 | cGTGTTCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGc > 2:1552801/1‑76 (MQ=255) tgtTCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCAt > 2:12342/1‑76 (MQ=255) gtTCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATg > 2:1552002/1‑76 (MQ=255) ttCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATgg < 2:1503124/76‑1 (MQ=255) ttCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATgg > 1:1637053/1‑76 (MQ=255) gTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCAc > 2:299877/1‑76 (MQ=255) gTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCAc < 1:141159/76‑1 (MQ=255) ggTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCa < 2:1565310/68‑1 (MQ=255) ggTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCa > 1:1565310/1‑68 (MQ=255) ggTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCg > 1:617647/1‑76 (MQ=255) tAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGAt < 2:897854/76‑1 (MQ=255) tAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGAt > 1:281481/1‑76 (MQ=255) tAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGAt > 1:169480/1‑76 (MQ=255) aGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGAtt > 1:839301/1‑76 (MQ=255) gAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATtc < 1:1071360/76‑1 (MQ=255) aaaCGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATtca < 2:1485674/76‑1 (MQ=255) aaCGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATtcat < 1:329904/76‑1 (MQ=255) cGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATtcatca < 2:1087928/76‑1 (MQ=255) cTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATcacc > 2:250164/1‑76 (MQ=255) tttCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATcacca < 1:1395587/76‑1 (MQ=255) ttCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATcaccac > 1:9777/1‑76 (MQ=255) tCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACt < 1:1022397/76‑1 (MQ=255) cTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCGGCATGGCACCGATTCATCACCACTa > 1:110792/1‑76 (MQ=255) cTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTa > 1:1584183/1‑76 (MQ=255) tGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTAt < 2:436453/76‑1 (MQ=255) gTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATg < 2:1269236/76‑1 (MQ=255) gTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATg < 1:1400739/76‑1 (MQ=255) gTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATg > 1:455382/1‑76 (MQ=255) tCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGa > 1:1297335/1‑76 (MQ=255) tCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGa > 2:1163415/1‑76 (MQ=255) aTCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAAc > 1:1283304/1‑76 (MQ=255) aTCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAAc > 2:1398513/1‑76 (MQ=255) tCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACt < 1:110558/76‑1 (MQ=255) cTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGa < 2:1689045/76‑1 (MQ=255) cTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGa > 1:1145770/1‑76 (MQ=255) gCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGaaa > 1:740506/1‑76 (MQ=255) gCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGaaa > 1:1462150/1‑76 (MQ=255) gCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGaaa > 1:1224368/1‑76 (MQ=255) cAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGAAAg > 2:1453247/1‑76 (MQ=255) aGGTCGGCTCCTTTAAACTGTGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGAAAgg > 2:712620/1‑76 (MQ=255) tCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTg < 2:1183295/76‑1 (MQ=255) tCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTg < 2:389408/76‑1 (MQ=255) cGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTgg < 1:663613/76‑1 (MQ=255) cGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTgg > 1:1269420/1‑76 (MQ=255) cGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTgg < 2:7710/76‑1 (MQ=255) cGGCTCCTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTgg > 1:494778/1‑76 (MQ=255) cTTTAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGaa < 2:1005198/76‑1 (MQ=255) ttAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAAcc < 1:1734400/76‑1 (MQ=255) tAAACTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACcg < 2:682403/76‑1 (MQ=255) cTGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACcgcgcg < 2:1289461/76‑1 (MQ=255) tGCGCGGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGt > 1:1233578/1‑76 (MQ=255) cgcgGACAACGTATTTGCAGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCa < 1:370574/76‑1 (MQ=255) cgGACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCAtt > 1:347915/1‑76 (MQ=255) gACAACGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATtgt < 2:1347012/76‑1 (MQ=255) aaCGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCg > 2:1282279/1‑76 (MQ=255) cGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGtt < 2:393262/76‑1 (MQ=255) cGTATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGtt > 1:1181637/1‑76 (MQ=255) tATTTTCAGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGTTTc < 2:1494572/76‑1 (MQ=255) tttCAGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGTTTCTgg < 2:1359762/76‑1 (MQ=255) | CGTGTTCGTGGTAGAAACGCTTTCTGTCATCCTGCAGGTCGGCTCCTTTAAACTGCGCGGACAACGTATTTTCCGCATGGCACCGATTCATCACCACTATGAACTGAAAGGCTGGCCGGAACCGCGCGTCATTGTGCGTTTCTGG > REL606/99690‑99834 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |