| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,145,932 | C→T | T23T (ACC→ACT) | rpoD → | RNA polymerase sigma factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,145,932 | 0 | C | T | 97.3% | 281.2 / ‑0.5 | 73 | T23T (ACC→ACT) | rpoD | RNA polymerase sigma factor |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (36/35); total (36/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01 | |||||||||||
TCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATG > REL606/3145860‑3146004 | tCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTAt < 2:1110560/76‑1 (MQ=255)tCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTAt < 1:739947/76‑1 (MQ=255) cTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATg > 1:1277148/1‑76 (MQ=255) ggAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAg > 2:571385/1‑76 (MQ=255) ggAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAg < 1:405677/76‑1 (MQ=255) gAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAgg < 2:1505796/76‑1 (MQ=255) cAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCa < 1:573079/76‑1 (MQ=255) aaaaCCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCaa > 2:104271/1‑76 (MQ=255) aCCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGa < 2:1174033/76‑1 (MQ=255) cccGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGAc > 1:1821564/1‑76 (MQ=255) ccGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGAcc > 1:674083/1‑76 (MQ=255) cGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCa < 2:940704/76‑1 (MQ=255) cAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATc > 2:59577/1‑76 (MQ=255) cAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATc > 2:1441903/1‑76 (MQ=255) gTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTg < 2:49559/76‑1 (MQ=255) gTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTg < 2:363302/76‑1 (MQ=255) gTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTg < 2:25338/76‑1 (MQ=255) gTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTg > 1:1547704/1‑76 (MQ=255) gTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTg < 1:545753/76‑1 (MQ=255) gTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTg < 2:1375540/76‑1 (MQ=255) gTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTg < 1:723695/76‑1 (MQ=255) cacaGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGcc > 1:717373/1‑76 (MQ=255) cTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAg > 2:1774941/1‑76 (MQ=255) tGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGa > 1:1739920/1‑76 (MQ=255) tGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGa > 2:1045662/1‑76 (MQ=255) gAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATCCCTAGCTCAATGACCATCTGCCGGAAGat > 1:1606635/1‑76 (MQ=255) aaaCTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGata > 2:643080/1‑76 (MQ=255) aaaCTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGata < 1:1031934/76‑1 (MQ=255) cttcttGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATAtcg < 1:330972/76‑1 (MQ=255) cttcttGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATAtcg > 1:1777776/1‑76 (MQ=255) cttcttGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATAtcg > 2:1663935/1‑76 (MQ=255) cttGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATAtcgtcg > 2:894337/1‑76 (MQ=255) cttGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATAtcgtcg < 1:1454766/76‑1 (MQ=255) cttGTCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATAtcgtcg > 2:868805/1‑76 (MQ=255) tCACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtc > 1:339858/1‑76 (MQ=255) cACCCGTGGTAAGGAGCAAGGCTATCTGGCTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtca < 2:808277/76‑1 (MQ=255) cACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtca < 1:702483/76‑1 (MQ=255) cACCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtca < 1:948424/76‑1 (MQ=255) aCCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtcag < 1:825276/76‑1 (MQ=255) aCCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtcag < 1:825297/76‑1 (MQ=255) aCCCGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtcag < 1:114444/76‑1 (MQ=255) ccGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtcagat < 2:1234059/76‑1 (MQ=255) ccGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtcagat < 2:1234070/76‑1 (MQ=255) cGTGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtcagatc < 2:1679623/76‑1 (MQ=255) tGGTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtcagatcag > 1:1277483/1‑76 (MQ=255) ggTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtcagatcaga > 1:1471538/1‑76 (MQ=255) gTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTcagatcagat < 2:1230601/76‑1 (MQ=255) gTAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTcagatcagat > 2:1525011/1‑76 (MQ=255) tAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCagatcagatc < 1:1454492/76‑1 (MQ=255) tAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCagatcagatc < 1:244249/76‑1 (MQ=255) tAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCagatcagatc < 2:128818/76‑1 (MQ=255) tAAGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCagatcagatc < 2:905223/76‑1 (MQ=255) aaGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGCTTCAGATCAGATCg > 2:842506/1‑76 (MQ=255) aaGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCg > 2:842485/1‑76 (MQ=255) aaGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCg < 1:522958/76‑1 (MQ=255) aGGAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGa > 2:498730/1‑76 (MQ=255) gAGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAg > 2:437656/1‑76 (MQ=255) aGCAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGa < 1:168720/76‑1 (MQ=255) cAAGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGAca > 2:483531/1‑76 (MQ=255) cAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGAca < 2:1584708/76‑1 (MQ=255) aaGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGAcat > 2:592959/1‑76 (MQ=255) aaGGCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGAcat < 1:1186192/76‑1 (MQ=255) gCTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGAcatcat < 1:1110436/76‑1 (MQ=255) cTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACatcatc > 2:1470304/1‑76 (MQ=255) cTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACatcatc > 2:1116806/1‑76 (MQ=255) cTATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACatcatc > 1:779261/1‑76 (MQ=255) tATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATcc > 2:414022/1‑76 (MQ=255) tATCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATcc > 1:1432556/1‑76 (MQ=255) aTCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGACATCGTCGATTCAGATCAGATCGAAGACATCATCCa > 1:979692/1‑76 (MQ=255) tCTGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCaa > 1:886695/1‑76 (MQ=255) tGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAAt > 2:1271291/1‑76 (MQ=255) tGACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAAt < 1:882581/76‑1 (MQ=255) gACTTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATg < 1:206836/76‑1 (MQ=255) | TCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATG > REL606/3145860‑3146004 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |