| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,928,833 | T→C | V206A (GTT→GCT) | rho → | transcription termination factor Rho |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,928,833 | 0 | T | C | 98.0% | 193.5 / ‑4.1 | 49 | V206A (GTT→GCT) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (29/19); total (30/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01 | |||||||||||
AAGCCGGTAAAACCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTC > REL606/3928758‑3928905 | aaGCCGGTAAAACCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGt > 2:1686747/1‑76 (MQ=255) ccGGTAAAACCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGctct > 1:46537/1‑76 (MQ=255) gTAAAACCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTctgct < 1:596285/76‑1 (MQ=255) gTAAAACCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTctgct < 1:596297/76‑1 (MQ=255) tAAAACCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTctgctg > 1:632138/1‑76 (MQ=255) aaaaCCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGa > 1:913870/1‑76 (MQ=255) aaCCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATc > 1:651202/1‑76 (MQ=255) aCCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATcg < 1:1441294/76‑1 (MQ=255) aTGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATcgacg < 1:1735032/76‑1 (MQ=255) aTGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATcgacg > 2:1430147/1‑76 (MQ=255) gctgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGaa < 1:1500281/76‑1 (MQ=255) gctgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGaa < 2:1693156/76‑1 (MQ=255) gctgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGaa < 1:279205/76‑1 (MQ=255) ctgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAAc > 1:1413205/1‑76 (MQ=255) ctgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGAGTGTGTGCTGATGGCTCTGCTGATCGACGAAc < 1:1476436/76‑1 (MQ=255) tgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACg > 2:1528170/1‑76 (MQ=255) gctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGt > 2:1802546/1‑76 (MQ=255) ctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTc > 2:633475/1‑76 (MQ=255) cAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCgg > 2:441691/1‑76 (MQ=255) aaCATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGgaag > 2:1698610/1‑76 (MQ=255) aaCATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGgaag < 2:562922/76‑1 (MQ=255) tGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTa < 2:1413340/76‑1 (MQ=255) gCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTaa > 1:685072/1‑76 (MQ=255) cAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCg > 1:808938/1‑76 (MQ=255) agagCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCga > 1:1621264/1‑76 (MQ=255) gagCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCgag < 2:318107/76‑1 (MQ=255) gagCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCgag > 2:1158489/1‑76 (MQ=255) cATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATg < 2:1821536/76‑1 (MQ=255) ttGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCa > 1:1825252/1‑76 (MQ=255) ttGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCa > 1:1734511/1‑76 (MQ=255) tGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAg > 1:897780/1‑76 (MQ=255) cTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCg > 1:678736/1‑76 (MQ=255) aCAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCt < 1:1131924/76‑1 (MQ=255) aaCCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTgg < 1:1078129/76‑1 (MQ=255) accaccCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGt < 1:609709/76‑1 (MQ=255) accaccCAGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGt < 1:609708/76‑1 (MQ=255) caccCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTaa < 1:684341/76‑1 (MQ=255) caccCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTaa > 1:515705/1‑76 (MQ=255) accCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGGaaa > 2:1012464/1‑76 (MQ=255) cgtaTTGTGTGCTGATGACTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAgg < 2:431916/73‑1 (MQ=255) ggATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGt < 2:1471344/76‑1 (MQ=255) ggATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGt < 1:136073/76‑1 (MQ=255) aTTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGa > 2:853267/1‑76 (MQ=255) tgtgCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAgtt > 1:446922/1‑76 (MQ=255) gtgCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAgttg > 1:1406841/1‑76 (MQ=255) tgCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAgttgt > 2:978070/1‑76 (MQ=255) tgCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAgttgt > 2:1723289/1‑76 (MQ=255) gCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAgttgtt > 2:463379/1‑76 (MQ=255) cTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGGGAAGttgttg > 1:386551/1‑76 (MQ=255) tGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGc > 2:90718/1‑76 (MQ=255) gATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCt > 1:149894/1‑76 (MQ=255) tGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTc > 2:1244189/1‑76 (MQ=255) | AAGCCGGTAAAACCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTC > REL606/3928758‑3928905 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |