| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,202,158 | A→C | I134M (ATT→ATG) | iclR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,202,158 | 0 | A | C | 97.6% | 168.1 / NA | 42 | I134M (ATT→ATG) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (18/23); minor base T (0/1); total (18/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATT > REL606/4202085‑4202229 | aaGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGatcat > 1:513047/1‑76 (MQ=255)aaGCGGGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGatcat < 1:195020/76‑1 (MQ=255) ggggCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATcataat < 1:59335/73‑1 (MQ=255) cGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATcataat > 2:1043790/1‑76 (MQ=255) tGCATCGGCAATTTCCCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCg < 1:87489/76‑1 (MQ=255) cATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCt > 1:566822/1‑76 (MQ=255) cATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCt > 2:446110/1‑76 (MQ=255) cGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCg < 2:1727763/76‑1 (MQ=255) ttttACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATc < 1:97834/75‑1 (MQ=255) tttACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCg > 2:1107766/1‑76 (MQ=255) tttACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCg > 2:13935/1‑76 (MQ=255) ccgccgATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCttt < 1:1578507/76‑1 (MQ=255) cgccgATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTg < 2:307607/76‑1 (MQ=255) cgccgATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTg < 1:137205/76‑1 (MQ=255) ccgATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGAt < 2:1338436/76‑1 (MQ=255) ccgATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGAt < 2:1322749/76‑1 (MQ=255) cgATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATc > 2:371234/1‑76 (MQ=255) gATAGGGGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCa < 1:1424889/76‑1 (MQ=255) gATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCa < 1:1049518/76‑1 (MQ=255) gATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCa < 2:996305/76‑1 (MQ=255) aTAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCaa < 1:90492/76‑1 (MQ=255) ggCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCa < 2:442047/76‑1 (MQ=255) ggCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCa > 1:989856/1‑76 (MQ=255) gcgcGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCAc < 2:1435255/76‑1 (MQ=255) cgcgGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCAcc < 2:806808/76‑1 (MQ=255) cgGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGc < 2:1345783/76‑1 (MQ=255) ggACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGcc < 2:595540/76‑1 (MQ=255) gACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa > 2:454145/1‑76 (MQ=255) gACATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCa < 1:758001/76‑1 (MQ=255) aCATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCat < 1:426552/76‑1 (MQ=255) cATTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCata > 1:1352591/1‑76 (MQ=255) aTTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCatat < 1:1692992/76‑1 (MQ=255) aTTCGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCatat > 1:655420/1‑76 (MQ=255) cGCATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGa < 2:133057/76‑1 (MQ=255) cATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGAcc > 1:1555680/1‑76 (MQ=255) cATCAGATGCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGAcc > 2:1342474/1‑76 (MQ=255) gCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGcc < 1:989368/76‑1 (MQ=255) gCGTACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGcc < 1:687698/76‑1 (MQ=255) gTACGCTGTCCCGGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAg < 1:776471/76‑1 (MQ=255) gTACACTGTCCCGGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAg < 1:731149/76‑1 (MQ=255) tACACTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGa > 1:1307585/1‑76 (MQ=255) acacTGTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGAc > 1:432548/1‑76 (MQ=255) cacTTTCCCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGAct < 2:1748000/76‑1 (MQ=255) cacTTTACTTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGAct < 1:1160722/76‑1 (MQ=255) cTGTCCCTGGTCGATCATATTCGCTTCGTGATCGTTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGActct < 1:1700267/76‑1 (MQ=255) gTACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTc > 1:589403/1‑76 (MQ=255) tACCTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTcc > 2:1229478/1‑76 (MQ=255) cccTGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCCCCAGACTCTTCCa < 1:1606553/75‑1 (MQ=255) ccTGGTCGATTATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCAt < 2:510029/76‑1 (MQ=255) tGGTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTa < 2:432548/76‑1 (MQ=255) tGGTCGATCATAACCGCTTCGTAATCGCTTTGATAAAGCCCCGCCATATTGACCGTTTCGCCAGACTCTTCCATTa < 1:416933/76‑1 (MQ=255) ggTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTaa > 2:1123314/1‑76 (MQ=255) ggTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTaa < 2:1273582/76‑1 (MQ=255) gTCGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTaaa > 1:925537/1‑76 (MQ=255) cGATCATAATCGCTTCGTTATCCCTTTGAACAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAAAtt < 2:1765064/76‑1 (MQ=255) cGATCATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAAAtt > 2:1410642/1‑76 (MQ=255) | AAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATT > REL606/4202085‑4202229 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |