| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,104,916 | A→T | D409E (GAT→GAA) | ycdR ← | predicted enzyme associated with biofilm formation |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,104,916 | 0 | A | T | 94.6% | 213.1 / 2.1 | 56 | D409E (GAT→GAA) | ycdR | predicted enzyme associated with biofilm formation |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base T (21/32); total (23/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCA > REL606/1104843‑1104988 | ggTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTc < 2:1626383/76‑1 (MQ=255) gTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCt < 2:1811430/76‑1 (MQ=255) gTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCt > 1:1233809/1‑76 (MQ=255) gTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCt < 1:505406/76‑1 (MQ=255) ttGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATcc < 1:280871/76‑1 (MQ=255) ttCAGGATGAATTTGTGCTTTTTTCTCCTCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAg < 2:96485/76‑1 (MQ=255) ttCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAg > 2:1528637/1‑76 (MQ=255) ttCAGGATGAATTTGTGCTTTTTTCTCCCCTGCTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAg > 2:661794/1‑76 (MQ=255) gATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTaa < 2:544040/76‑1 (MQ=255) aTGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAAt > 2:139803/1‑76 (MQ=255) tttGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCgg < 2:486584/76‑1 (MQ=255) tgtgCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCa < 1:1022875/76‑1 (MQ=255) tgtgCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCa > 1:1701597/1‑76 (MQ=255) tgtgCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCa < 1:119830/76‑1 (MQ=255) cTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCa > 2:160750/1‑76 (MQ=255) ttttttCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCAc < 2:630357/76‑1 (MQ=255) ttttCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGc > 2:1135943/1‑76 (MQ=255) tttCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCa > 2:346827/1‑76 (MQ=255) tctcCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATa < 1:994130/76‑1 (MQ=255) ctcCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAg < 1:123670/76‑1 (MQ=255) ctcCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAg < 2:1362334/76‑1 (MQ=255) ccccTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGAt > 2:766108/1‑76 (MQ=255) ccccTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGAt < 1:1322019/76‑1 (MQ=255) ccTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGt < 1:1042108/76‑1 (MQ=255) cTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGtt > 1:1265623/1‑76 (MQ=255) cTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGtt > 2:1699857/1‑76 (MQ=255) gTTGGTGAGTATTTTACTCGCGTTAATGTGGGTGCTAAATCCCAGCTTAAGACCGGCATCCACGCATAGATGTTTa < 1:1204130/76‑1 (MQ=255) gTTGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTa < 1:1204147/76‑1 (MQ=255) ttGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTac < 2:1082480/76‑1 (MQ=255) tGGTAGGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTaca < 2:689678/76‑1 (MQ=255) tGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCGACGCATAGATGTTTaca < 2:108869/76‑1 (MQ=255) tGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTacc > 1:1640384/1‑75 (MQ=255) tGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTaca > 1:1573750/1‑76 (MQ=255) tGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTaca > 1:1254753/1‑76 (MQ=255) tGGTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTaca > 1:1640370/1‑76 (MQ=255) gTAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACAcc > 2:1475372/1‑76 (MQ=255) gTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACAcc > 1:398236/1‑76 (MQ=255) tAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCt > 2:1035070/1‑76 (MQ=255) tAAGTATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCt > 2:455171/1‑76 (MQ=255) tATTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGc < 1:1449550/76‑1 (MQ=255) aTTTTACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCg < 1:595955/76‑1 (MQ=255) ttttACTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCgg < 2:669495/76‑1 (MQ=255) aCTCGCGTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTAc > 2:850203/1‑76 (MQ=255) tCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGt < 1:1353706/76‑1 (MQ=255) cgcgTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTa < 2:563580/76‑1 (MQ=255) cgTTAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAAt < 1:214485/76‑1 (MQ=255) tAATGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGc > 1:1019367/1‑76 (MQ=255) aTGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCa < 2:1502061/76‑1 (MQ=255) aTGTGGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCa < 2:425882/76‑1 (MQ=255) tgtgGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAg < 1:1153931/76‑1 (MQ=255) tgtgGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAg < 2:253726/76‑1 (MQ=255) tgtgGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAg < 2:1711840/76‑1 (MQ=255) tgtgGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAg < 1:753832/76‑1 (MQ=255) tgtgGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGCACGTAATTGCCAg > 2:948546/1‑76 (MQ=255) tgGGTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGc > 2:584537/1‑76 (MQ=255) gggTTCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCa < 1:469852/76‑1 (MQ=255) | GGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCA > REL606/1104843‑1104988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |