| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,175,232 | (T)6→7 | coding (877/933 nt) | pbpG ← | D‑alanyl‑D‑alanine endopeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,175,226 | 1 | . | T | 97.1% | 130.5 / ‑3.4 | 35 | coding (883/933 nt) | pbpG | D‑alanyl‑D‑alanine endopeptidase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (13/21); total (14/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCG‑CCGCCATTT‑GGGCGG‑C‑TTTTT‑G‑TTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCG > REL606/2175152‑2175295 | cAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCG‑CCGCCCTTT‑TGGCGG‑G‑TTTTT‑G‑t > 2:980414/1‑76 (MQ=255)cAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCGGCCCCGCCG‑CCGCCATTT‑GGGCGG‑C‑TTTTT‑G‑t < 1:558840/76‑1 (MQ=255) gTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCG‑CCGCCATTT‑GGGCGG‑C‑TTTTT‑G‑ttttt < 2:1728889/76‑1 (MQ=255) aTCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCG‑CCGCCATTT‑GGGCGG‑C‑TTTTT‑G‑ttttttt < 2:206701/76‑2 (MQ=255) gCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCCCCCCTTT‑GGGGGG‑G‑ttttt‑t‑ttttttttt > 1:1646963/1‑73 (MQ=255) cATCCGATTTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCG‑CCGCCATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGc < 1:1434882/76‑1 (MQ=255) ccGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCG‑CCGCCATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCa < 2:520747/76‑1 (MQ=255) ccGAATTCACTTAATCGTTCTGTGCCGCCTGCCCCGCCG‑CCGCCATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCa < 1:782233/76‑1 (MQ=255) aatttCCCTTATCCGTGCTGTGCCGTCGGCCCCGCCG‑CCGCCATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAAc < 1:575651/73‑1 (MQ=255) aaTTCACTTAGTCGTTCTGTGCCTTCGCCCCCGCCG‑CCGCCATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACg < 2:1685760/76‑1 (MQ=255) tCACTTAATCTTTCTGTGCCGTCTGCCCCGCCG‑CCGCCATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACgctg < 2:629987/76‑1 (MQ=255) tCACTTAATCGTTCTGTGCCGTCTGCCCCGCCG‑CCGCCCTTT‑GGGGGG‑CTTTTTT‑T‑TTTTTTTTTGCTCAACgctg > 1:1894933/1‑76 (MQ=255) cTTAATCGTTCTGTGCCGTCTGCCCCGCCG‑CCGCCATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACGctgctg < 2:2055809/76‑1 (MQ=255) aaTCTTTCTGTGCCGTCTGCCCCGCCG‑CCGCCATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCg < 1:733655/76‑1 (MQ=255) tgtgCCGTCTGCCCCGCCGCCCCCCCTTT‑GGGGGC‑T‑TTTTT‑T‑TTTTTTTTTGCTCAACGCTGCTGCCGGCCCAGGc > 2:1611257/1‑76 (MQ=255) tgtgCCGTCTGCCCCGCCG‑CCGCCATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGc > 2:385965/1‑76 (MQ=255) gggCCGTCGGCCCCGCCG‑CCGCCATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCa < 2:1609173/74‑1 (MQ=255) ccGTCTGCCCCGCCG‑CCGCCATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGGATCa > 2:1805663/1‑76 (MQ=255) ccGTCTGCCCCGCCG‑CCCCCCTTTGGGGGGG‑T‑TTTTT‑T‑TTTTTTTTAGCTCAACGCTGCTGCCGGCACAGGCATCa > 2:1871884/1‑76 (MQ=255) ccGTCTGCCCCGCCG‑CCCCCCCTT‑TGGCGGCC‑TTTTT‑T‑TTTTTTTTTTTTCAACCCTTCTGCCCGCCCACGCCTCa > 2:317158/1‑76 (MQ=255) ccGTCTGCCCCGCCC‑CCCCCATTT‑TGGCGG‑G‑TTTTTGG‑TTTTTTTTAGCTCAAAGCTGCTGCCTGCACAGGCATCa > 2:183861/1‑76 (MQ=255) ccgCCCCGCCG‑CCGCCATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACttt < 1:454349/74‑1 (MQ=255) tcccccGCCG‑CCGCCATTG‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTa < 1:940951/74‑1 (MQ=255) ccccGCCG‑CCGCCCTTT‑TGGCGG‑C‑TTTTT‑GTTTTTTTATAATTCTACCAGGCTGCAGGCACAGGGAACACCTTAcc > 2:768045/1‑76 (MQ=255) cccGCCG‑CCGCCATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCg > 2:500802/1‑76 (MQ=255) cccccg‑ccgccATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCgg < 1:1322688/73‑1 (MQ=255) cgccg‑ccgcCATTT‑TGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAATGCTGCAGCCGGCCCAGGCATCACTTTACCGGt > 2:664863/1‑76 (MQ=255) cgccg‑ccgcCATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGt > 2:746078/1‑76 (MQ=255) cgccg‑ccgcCATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGAATAACTTTACCGGt > 2:32293/1‑76 (MQ=255) gccg‑ccgccATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGtt > 2:1191074/1‑76 (MQ=255) gccg‑ccgccATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGtt > 2:1236041/1‑76 (MQ=255) ccg‑ccgccATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCCCAGGCATCACTTTACCGGttt > 2:1307950/1‑76 (MQ=255) cg‑ccgccATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTc < 2:1289028/76‑1 (MQ=255) g‑ccgccATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCa < 2:274567/76‑1 (MQ=255) ccATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATcc > 2:1379177/1‑76 (MQ=255) ccATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATcc < 1:2004917/76‑1 (MQ=255) cATTT‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCa < 2:1093466/76‑1 (MQ=255) ttt‑GGGCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAgg < 1:1436315/76‑1 (MQ=255) gggCGG‑C‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTAc > 2:1075354/1‑76 (MQ=255) ggcgg‑c‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACg < 1:3422/76‑1 (MQ=255) ggcgg‑c‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACg < 2:461207/76‑1 (MQ=255) cgg‑c‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCa > 2:654474/1‑76 (MQ=255) cgg‑c‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCa > 1:1489303/1‑76 (MQ=255) gg‑c‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAg < 1:742522/76‑1 (MQ=255) g‑c‑TTTTT‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAgg < 1:565863/76‑1 (MQ=255) ttttt‑GTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAggcg > 2:779480/1‑76 (MQ=255) | CAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCG‑CCGCCATTT‑GGGCGG‑C‑TTTTT‑G‑TTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCG > REL606/2175152‑2175295 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |