Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	REL606	2,103,918	(CCAG)7→9	coding (185/216 nt)	ECB_01992 →	hypothetical protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	2103888 =	9 (0.140)	38 (0.850)	30/104	0.4	92.5%	coding (155/216 nt)	ECB_01992	hypothetical protein
	?	REL606	= 2103895	0 (0.000)					coding (162/216 nt)	ECB_01992	hypothetical protein

TTTTTAGTGTTGCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/2103985‑2103888 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctggctggctggctggctggctgGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTCATTGTATA  <  REL606/2103895‑2103822                                                                                                                                                                              TTTTTAGTGTTGCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACGCCTGGCTGGC                                                                                                  >  1:34695/1‑76  TTTTAGTGTTGCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCT                                                                                                 >  2:959062/1‑76   nTTAGTGTTGCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTCGCTG                                                                                                >  1:558820/2‑76   TTTAGTGTTGCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTG                                                                                                <  1:1093877/76‑1   TTTAGTGTTGCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTG                                                                                                >  1:1982543/1‑76    TTAGTGTTGCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGG                                                                                               >  2:340227/1‑76      AGTGTTGCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCT                                                                                             >  1:660317/1‑76       GTGTTGCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTG                                                                                            <  1:1563745/76‑1          TTGCCTTTTATAGTATAATAAGGGAAATTATTTCGTAGTACAAAATCCACATACACACCTGGCTGGCTGGATGGCT                                                                                         >  2:235972/1‑76            GCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACTTGGCTGGCTGGCTGGCTGG                                                                                       >  1:1841314/1‑76            GCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGG                                                                                       <  1:1073763/76‑1            GCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGG                                                                                       >  1:680818/1‑76             CCTTTTATAGTATAATAGGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGC                                                                                      >  1:672095/1‑76                TTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGG                                                                                   <  1:1743240/76‑1                 TTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGC                                                                                  <  1:1860491/76‑1                  TATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCT                                                                                 >  1:626423/1‑76                    TAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGG                                                                               <  2:1099596/76‑1                       TATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTG                                                                            >  1:744388/1‑76                        ATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                                                                           <  2:1469154/76‑1                        ATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                                                                           >  1:1284101/1‑76                         TAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGC                                                                          >  2:47938/1‑76                          AATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCT                                                                         >  1:12085/1‑76                          AATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCT                                                                         >  1:1531474/1‑76                           ATAAGGGCAACTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTG                                                                        <  1:106945/76‑1                             AAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGg                                                                      >  1:860811/1‑75                                GGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGT                                                                   >  2:252407/1‑76                                 GCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTG                                                                  <  1:493916/76‑1                                   AATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAA                                                                <  2:1137500/76‑1                                       ATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGTTGTGGAATCa                                                            >  2:141159/1‑75                                       ATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCT                                                            >  1:326386/1‑76                                        TTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCAGGCTGGCTGGCTGTGAAATCTA                                                           >  1:925166/1‑76                                           CGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGGAATCTAATG                                                        >  1:813246/1‑76                                           CGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATG                                                        >  2:1250636/1‑76                                           CGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATG                                                        >  2:1967509/1‑76                                           CGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATG                                                        <  1:1701724/76‑1                                            GTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGC                                                       <  1:801834/76‑1                                              AGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAA                                                     >  2:1141773/1‑76                                                TACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAAT                                                   <  2:1003290/76‑1                                                TACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAAT                                                   <  1:1927500/76‑1                                                TACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAAT                                                   >  1:1930990/1‑76                                                    AAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCA                                               <  1:132645/76‑1                                                    AAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCA                                               >  2:1047339/1‑76                                                     AATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCAC                                              <  2:1457373/76‑1                                                         CACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCT                                          >  2:920669/1‑76                                                         CACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCT                                          >  2:1567978/1‑76                                                         CACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCT                                          >  2:2102823/1‑76                                                           CATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTT                                        <  2:1518178/76‑1                                                            ATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTA                                       <  1:470364/76‑1                                                            ATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTA                                       >  2:1719373/1‑76                                                              TCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATA                                     <  2:803859/76‑1                                                               CACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCCGGGCAATCTAATGCAAATTTCCCCCCCTTCTAT                                    >  2:1671031/1‑76                                                                   CCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGA                                <  2:984003/76‑1                                                                     TGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAG                              >  2:747265/1‑76                                                                      GGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGT                             <  2:876536/76‑1                                                                      GGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGT                             <  2:713538/76‑1                                                                        CTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCA                           <  2:813246/76‑1                                                                             TGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAAC                      <  2:660317/76‑1                                                                              GGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACG                     <  1:747265/76‑1                                                                                 TGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAG                  <  2:363784/76‑1                                                                                   GCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCA                <  1:963791/76‑1                                                                                     TGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGT              >  2:1192181/1‑76                                                                                        CTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTT           >  1:469863/1‑76                                                                                        CTGGCTGGCTGGCTAGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTT           >  2:1499848/1‑76                                                                                         TGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTC          <  1:541964/76‑1                                                                                              GGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTCATTGT     <  2:838416/76‑1                                                                                              GGCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTCATTGT     <  2:259557/76‑1                                                                                               GCTGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTCATTGTA    <  1:1141773/76‑1                                                                                                 TGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTCATTGTATA  <  2:1841314/76‑1                                                                                                 TGGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTCATTGTATA  <  1:226035/76‑1                                                                                                                                                                              TTTTTAGTGTTGCCTTTTATAGTATAATAAGGGCAATTATTTCGTAGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/2103985‑2103888 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctggctggctggctggctggctgGCTGGCTGTGAAATCTAATGCAAATTTCACCCCTTTATATTCGAAGTCAATAACGAAGCAGTTTTCATTGTATA  <  REL606/2103895‑2103822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.