| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,747,903 | 0 | G | T | 22.7% | 286.1 / 59.5 | 97 | A8S (GCA→TCA) | ydiL | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (51/24); new base T (22/0); total (73/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.31e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 111 total aligned reads displayed.
CAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGC > REL606/1747828‑1747977 | cagGCTGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAgg > 2:2040050/1‑76 (MQ=255) agGCTGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGc < 2:198012/76‑1 (MQ=255) agGCTGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGc < 1:837358/76‑1 (MQ=255) ggCTGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGTTTATGAACTCCAGGCa < 1:927601/76‑1 (MQ=255) ggCTGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCa > 1:418661/1‑76 (MQ=255) ggCTGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCa < 1:641015/76‑1 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAc > 1:1937858/1‑76 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAc > 1:430636/1‑76 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCAc < 2:739755/76‑1 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCAc < 2:70250/76‑1 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCAc < 2:1738639/76‑1 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcg > 1:169039/1‑75 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcc > 1:741692/1‑75 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACt > 1:922559/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACt > 2:1536445/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCATATGAACTCCAGTCAca > 1:1218120/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcca > 1:1017063/1‑74 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcca > 1:1912303/1‑74 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACta > 1:963528/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACta > 1:1825760/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACta > 1:1951462/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACta > 1:137282/1‑75 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcctt > 1:89907/1‑73 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAccag > 1:1746887/1‑73 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAccag > 1:1960385/1‑73 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcact > 1:1713401/1‑73 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcact > 1:273041/1‑73 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtag > 1:1446743/1‑74 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtagc > 1:1244968/1‑73 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtagc > 1:1395881/1‑73 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCg < 2:107331/76‑1 (MQ=255) tAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGc > 1:294104/1‑76 (MQ=255) aaTGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGcc < 1:766357/76‑1 (MQ=255) aaTGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGcc < 2:1683904/76‑1 (MQ=255) aaTGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGcc > 1:1801917/1‑76 (MQ=255) aTGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCa > 2:778841/1‑76 (MQ=255) gTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCata > 2:1364270/1‑76 (MQ=255) gTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCata > 1:627695/1‑76 (MQ=255) ttatttatATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCatat > 1:1926849/1‑76 (MQ=255) ttatttatATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCatat > 2:1837588/1‑76 (MQ=255) ttatttatATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCatat < 2:1326779/76‑1 (MQ=255) ttatttatATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCatat > 1:9939/1‑76 (MQ=255) tatttatATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAtt > 1:235206/1‑76 (MQ=255) atttatATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAttt > 2:710362/1‑76 (MQ=255) tttATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATg > 1:1671028/1‑76 (MQ=255) tATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGAc > 2:520935/1‑76 (MQ=255) tttGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACt < 1:1757650/75‑1 (MQ=255) aTTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACt < 2:2102526/76‑1 (MQ=255) tGTTTTTTTCTTTGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACtc > 2:895712/1‑75 (MQ=255) tGTTTTTTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAt < 1:1167834/76‑1 (MQ=255) tGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAt > 2:1329469/1‑76 (MQ=255) gTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAtt > 1:1457873/1‑76 (MQ=255) tttATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtg < 1:1350898/76‑1 (MQ=255) tttATTTCA‑GGCAGCCATTACACGATAACCGCTTATGACCCCCAGGCACTCCGCCATATTTTTGCCATGACTATtg < 1:1287665/76‑1 (MQ=255) ttATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtga > 1:477783/1‑76 (MQ=255) ttATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtga > 2:1747636/1‑76 (MQ=255) ttATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtga > 2:1692972/1‑76 (MQ=255) tATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgat > 1:1224891/1‑76 (MQ=255) tATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgat > 1:1391486/1‑76 (MQ=255) tATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgat > 1:1282769/1‑76 (MQ=255) ttttCA‑TCCACCCATTACACGATAACCGTTTATAACCCCCAGGCACTCCGCCATATTTTTGCCATGACTATtgatg < 1:552245/75‑1 (MQ=255) aTTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgatg > 1:1568205/1‑76 (MQ=255) aTTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgatg > 1:1228168/1‑76 (MQ=255) ttCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGaa > 1:8153/1‑76 (MQ=255) tCA‑TGCAGCCATTACACGATGAACGCTTATGAACCCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAAt < 1:688895/76‑1 (MQ=255) cA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATg > 2:641713/1‑76 (MQ=255) cA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATg > 2:1441725/1‑76 (MQ=255) cA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATg > 2:328933/1‑76 (MQ=255) a‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgc > 2:1534039/1‑76 (MQ=255) tGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgcg > 2:1963420/1‑76 (MQ=255) cAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGcgcg < 2:1152811/76‑1 (MQ=255) cAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGcgcg > 1:347176/1‑76 (MQ=255) cAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGcgcg > 2:1591162/1‑76 (MQ=255) cAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGcgcg > 2:1445390/1‑76 (MQ=255) gCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGAc < 2:1926849/76‑1 (MQ=255) ccATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGAcc > 1:1240069/1‑76 (MQ=255) cATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGTGCGACCt > 2:1327311/1‑76 (MQ=255) cATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCt > 1:1791080/1‑76 (MQ=255) cATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCt < 1:1499524/76‑1 (MQ=255) ttACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTgg > 2:127144/1‑76 (MQ=255) acacGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGAt > 2:352477/1‑76 (MQ=255) aTGAACGCTTATGACCCCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGccc < 1:167480/76‑1 (MQ=255) aTGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGccc > 2:169833/1‑76 (MQ=255) aaCGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGa > 1:1045428/1‑76 (MQ=255) aCGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGAc > 2:327470/1‑76 (MQ=255) gCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCg > 2:1342598/1‑76 (MQ=255) cTTATGAACTCCAGGCCCTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCgg < 1:2083720/76‑1 (MQ=255) tATGACCTCCGGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTg < 1:852434/76‑1 (MQ=255) tATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTg > 1:913829/1‑76 (MQ=255) tATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTg < 2:852106/76‑1 (MQ=255) tGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGAc < 2:1042467/76‑1 (MQ=255) tGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGAc > 1:1011739/1‑76 (MQ=255) tGAACCCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGAc < 2:1042483/76‑1 (MQ=255) cTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTg > 2:839264/1‑76 (MQ=255) ccAGGCCCTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGaa < 1:551834/76‑1 (MQ=255) ccAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGaa > 2:1454305/1‑76 (MQ=255) cAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGaaa < 2:1781849/76‑1 (MQ=255) ggCCCTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGc < 1:1142511/76‑1 (MQ=255) ggCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGc < 1:1661131/76‑1 (MQ=255) ggCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGc < 1:1546874/76‑1 (MQ=255) | CAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCA‑TGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGC > REL606/1747828‑1747977 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |