| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,492,082 | 0 | T | G | 35.5% | 150.8 / 21.1 | 76 | V490G (GTG→GGG) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (6/43); new base G (27/0); total (33/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.89e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCTG > REL606/3492009‑3492154 | gCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtggggg > 2:936030/1‑76 (MQ=255) ccTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtggggggg > 2:2069312/1‑74 (MQ=255) ccTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtggggggg > 1:668333/1‑74 (MQ=255) ccTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAg < 2:1559634/76‑1 (MQ=255) cTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtgggggggg > 2:1083649/1‑73 (MQ=255) cTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGt < 1:1084562/76‑1 (MQ=255) cTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGt < 2:528839/76‑1 (MQ=255) cTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAgg > 2:982882/1‑75 (MQ=255) cTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGt > 1:1571009/1‑76 (MQ=255) tgtgGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGGGta > 1:1258007/1‑76 (MQ=255) gtgGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGtat < 2:419881/76‑1 (MQ=255) tgGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGtata < 1:307897/76‑1 (MQ=255) tgGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGtata > 1:1068353/1‑76 (MQ=255) tgGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGgata > 1:354191/1‑76 (MQ=255) ggCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATAc > 1:1169311/1‑76 (MQ=255) ggCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGGATAc > 2:64784/1‑76 (MQ=255) cGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACgc < 2:1814755/76‑1 (MQ=255) cGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACgc > 1:1975050/1‑76 (MQ=255) gTCGCGCAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATACgca > 1:2105745/1‑76 (MQ=255) tCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACgcag < 1:1419096/76‑1 (MQ=255) tCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACgcag < 2:392463/76‑1 (MQ=255) cGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACgcagc < 1:1085251/76‑1 (MQ=255) cGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACgcagc < 2:1227824/76‑1 (MQ=255) cGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATACgcagc > 1:1514904/1‑76 (MQ=255) gCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCCTGTGAGTCAGTGGCTGGTGGAGTATACgcagca < 1:6645/76‑1 (MQ=255) cacaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGcagcag < 1:773980/76‑1 (MQ=255) cacaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGcagcag < 1:1083649/76‑1 (MQ=255) cacaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGcagcag < 1:1932698/76‑1 (MQ=255) cacaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATACGcagcag > 1:1781512/1‑76 (MQ=255) cacaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACGcagcag > 1:623443/1‑76 (MQ=255) cacaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGcagcag > 1:249648/1‑76 (MQ=255) acaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGa < 1:1509678/76‑1 (MQ=255) caAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGGGTATACGCAGCAGaa > 1:1179278/1‑76 (MQ=255) caAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGaa < 1:1204325/76‑1 (MQ=255) caAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGaa > 1:298899/1‑76 (MQ=255) aaaaCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAg < 2:1200404/76‑1 (MQ=255) aaaaCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAg < 1:1308105/76‑1 (MQ=255) aaaCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGc < 1:1847626/76‑1 (MQ=255) aaaCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGc < 1:562373/76‑1 (MQ=255) aaCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCt < 2:22780/76‑1 (MQ=255) aCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTg < 2:1872568/76‑1 (MQ=255) cAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACACAGGCGAAGCTGt > 2:1691358/1‑76 (MQ=255) cATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTg > 1:1004955/1‑76 (MQ=255) aTGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTgg < 2:283316/76‑1 (MQ=255) aTGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTgg > 1:658865/1‑76 (MQ=255) aTGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTgg > 1:793688/1‑76 (MQ=255) tgtgGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACGCAGCAGAAGCTCTCGGTggg > 2:375968/1‑75 (MQ=255) gtgGCTAAAGGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGcg < 1:375968/76‑1 (MQ=255) tgGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGcgt < 1:1212081/76‑1 (MQ=255) gCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGcgtcg < 2:1166365/76‑1 (MQ=255) cTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATGCGCAGCAGAAGCTGTCGCTGGcgtcgt < 1:1992046/76‑1 (MQ=255) cTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGcgtcgg > 1:104942/1‑75 (MQ=255) tAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTg < 1:132434/76‑1 (MQ=255) tAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTg < 2:127352/76‑1 (MQ=255) tAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTAGATGCAGCCGAAGCTGTCGCTGGcgtcggg > 2:127507/1‑74 (MQ=255) tAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGGATACGCAGCAGAAGCTGTCGCTGGCGGCGTg > 2:348300/1‑76 (MQ=255) aaaTGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGa < 2:156019/76‑1 (MQ=255) aaaTGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATACGCAGCAGAAGCTGTCGCTGgcggcgggg > 1:1462924/1‑73 (MQ=255) aTGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGAtt < 1:1180884/76‑1 (MQ=255) gCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTa < 1:1130588/76‑1 (MQ=255) cGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATACGCCGCGGAAGGTGTCGCTGGCGGGGTGAattta > 2:106397/1‑74 (MQ=255) cGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTaa > 1:2102521/1‑76 (MQ=255) tCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACGCAGCAGAAGCTGTCGCTGGCGTCGGGATTTAATGTa > 2:635608/1‑76 (MQ=255) tCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGGGGCGTGGTTTAATGTa > 2:1394239/1‑76 (MQ=255) cctacaaTCTCGTGTGAGTCAGTGGCTGGGGGGGTATACGCAGCAGAAGCTGTCGCTGGCGGCGTGATTTTATGTa > 2:818061/4‑76 (MQ=255) caacaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGGCGGGGTTTAATGTaa > 2:871304/1‑76 (MQ=255) caacaaTCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGCGGCGGGGTTTAATGTaa > 1:1268859/1‑76 (MQ=255) aacaaTCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGCGGCGTGGTTTTATGTAAg > 1:828381/1‑76 (MQ=255) acaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAgg < 1:1211787/76‑1 (MQ=255) aTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTgg < 2:1188552/76‑1 (MQ=255) aTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTgg < 2:1658312/76‑1 (MQ=255) aTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTgg < 2:2088321/76‑1 (MQ=255) ctcGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGcc < 2:1161229/76‑1 (MQ=255) tcGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCa < 1:1724805/76‑1 (MQ=255) cGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGCGGCGTGATTTAATGTAAGGGGGcccc > 1:1642965/1‑74 (MQ=255) tgtgAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGt > 2:355664/1‑76 (MQ=255) gAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAg < 2:1180240/76‑1 (MQ=255) cgccAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGa < 1:16242/73‑1 (MQ=255) gTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGGGGCCAGGTCAGAt > 2:639728/1‑76 (MQ=255) aGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTc < 1:455263/76‑1 (MQ=255) tGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCaa < 1:1500252/76‑1 (MQ=255) tGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCaa > 2:113930/1‑76 (MQ=255) gCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATc > 2:2002111/1‑76 (MQ=255) cTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCt > 2:608567/1‑76 (MQ=255) cTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCt < 1:1728606/76‑1 (MQ=255) cTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCt > 2:1108375/1‑76 (MQ=255) tggtggAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCTg > 1:45366/1‑76 (MQ=255) tggtggAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGGCAGGTTTTAATTTg > 2:794516/1‑76 (MQ=255) tggtggAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCACGCCAGACTTTAATCtt > 1:1002705/1‑75 (MQ=255) | GCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCTG > REL606/3492009‑3492154 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |