Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,290,641	C→A	C202* (TGC→TGA)	hnr →	response regulator of RpoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,290,641	0	C	A	100.0%	221.6 / NA	57	C202* (TGC→TGA)	hnr	response regulator of RpoS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/38);  total (19/38)

GTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTA  >  REL606/1290570‑1290713                                                                        |                                                                         gTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTg                                                                      <  2:1921724/76‑1 (MQ=255) gTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTg                                                                      <  2:375962/76‑1 (MQ=255)     ccGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGt                                                                  <  1:1744093/76‑1 (MQ=255)      cgcgGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTc                                                                 >  2:812808/1‑76 (MQ=255)       gcgGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCa                                                                <  1:474697/76‑1 (MQ=255)       gcgGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCa                                                                <  1:1670253/76‑1 (MQ=255)       gcgGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCa                                                                <  1:1491103/76‑1 (MQ=255)           aCAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCg                                                            <  1:1028499/76‑1 (MQ=255)           aCAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCg                                                            <  1:365991/76‑1 (MQ=255)              aaCCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGc                                                         >  2:18828/1‑76 (MQ=255)              aaCCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGc                                                         >  2:91911/1‑76 (MQ=255)                 ccGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTgg                                                      <  1:1395636/76‑1 (MQ=255)                 ccGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTgg                                                      <  1:1857661/76‑1 (MQ=255)                  cGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGa                                                     <  2:1129596/76‑1 (MQ=255)                     ccTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACAt                                                  >  1:583774/1‑76 (MQ=255)                      cTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATa                                                 <  2:399631/76‑1 (MQ=255)                      cTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATa                                                 <  1:609756/76‑1 (MQ=255)                       tGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATaa                                                >  2:409260/1‑76 (MQ=255)                       tGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATaa                                                <  2:1400558/76‑1 (MQ=255)                       tGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATaa                                                <  1:419588/76‑1 (MQ=255)                             ttGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGt                                          <  1:1323275/76‑1 (MQ=255)                               gATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTAc                                        >  1:1471230/1‑76 (MQ=255)                                 tatTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACtt                                      <  1:835274/76‑1 (MQ=255)                                 tatTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACtt                                      <  2:1828164/76‑1 (MQ=255)                                     gCCGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTtgctg                                  <  2:1474443/76‑1 (MQ=255)                                      ccGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTtgctgc                                 >  1:1126366/1‑76 (MQ=255)                                      ccGCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTtgctgc                                 >  1:1274562/1‑76 (MQ=255)                                        gCACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCt                               <  1:1233368/76‑1 (MQ=255)                                         cACTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCtt                              <  2:66125/76‑1 (MQ=255)                                           cTTTCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGt                            >  2:653470/1‑76 (MQ=255)                                            tttCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGtt                           <  2:1705612/76‑1 (MQ=255)                                            tttCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGtt                           <  1:1762263/76‑1 (MQ=255)                                            tttCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGtt                           <  1:1318033/76‑1 (MQ=255)                                             ttCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGtta                          >  2:617364/1‑76 (MQ=255)                                              tCGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGttat                         <  2:1588005/76‑1 (MQ=255)                                               cGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGttatt                        <  2:777461/76‑1 (MQ=255)                                               cGGAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGttatt                        <  1:867045/76‑1 (MQ=255)                                                 gAAAACGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTAc                      <  1:691801/76‑1 (MQ=255)                                                  aaaaCGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTAcg                     <  1:45133/76‑1 (MQ=255)                                                  aaaaCGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTAcg                     <  1:697514/76‑1 (MQ=255)                                                  aaaaCGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTAca                     <  2:130002/76‑2 (MQ=255)                                                   aaaCGATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTAcgc                    >  2:921042/1‑76 (MQ=255)                                                       gATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCAt                <  1:1694313/76‑1 (MQ=255)                                                       gATCTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCAt                <  1:1642564/76‑1 (MQ=255)                                                          cTGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGt             >  1:1147847/1‑76 (MQ=255)                                                           tGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGtt            >  2:1451259/1‑76 (MQ=255)                                                           tGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGtt            >  2:699279/1‑76 (MQ=255)                                                           tGGCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGtt            <  1:1430297/76‑1 (MQ=255)                                                             gCATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTa          >  2:1511037/1‑76 (MQ=255)                                                             gCATTTTACTGACTTGATGTCTCCCGAGCTGGTCATAATGGCGTACCTGCTGCCTTGTTATTACGCGCATTGTTTa          <  2:443251/76‑1 (MQ=255)                                                              cATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTaa         >  2:587500/1‑76 (MQ=255)                                                              cATTTTATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTaa         <  1:1646446/76‑1 (MQ=255)                                                                ttttATTGACTTGATGTCACCCGAGCTGGACATAATAGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACg       <  1:1762419/76‑1 (MQ=255)                                                                  ttATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGa     >  2:1734058/1‑76 (MQ=255)                                                                   tATTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGat    >  2:1855915/1‑76 (MQ=255)                                                                    aTTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGatt   >  1:77154/1‑76 (MQ=255)                                                                    aTTGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGatt   <  1:60073/76‑1 (MQ=255)                                                                     ttGACTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGatta  <  1:2013557/76‑1 (MQ=255)                                                                        |                                                                         GTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTA  >  REL606/1290570‑1290713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑