Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,570,276	T→C	D45G (GAT→GGT)	yhiO ←	universal stress protein UspB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,570,276	0	T	C	100.0%	312.5 / NA	74	D45G (GAT→GGT)	yhiO	universal stress protein UspB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/39);  total (35/39)

TTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGC  >  REL606/3570203‑3570348                                                                          |                                                                         ttGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTccacca                                                                        >  1:642854/1‑76 (MQ=255)   gggCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAAc                                                                      <  1:1655097/76‑1 (MQ=255)    ggCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACa                                                                     >  2:947732/1‑76 (MQ=255)    ggCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACa                                                                     >  2:947713/1‑76 (MQ=255)    ggCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACa                                                                     >  2:721528/1‑76 (MQ=255)      cATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACata                                                                   <  2:1680344/76‑1 (MQ=255)         aGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTg                                                                >  2:260252/1‑76 (MQ=255)         aGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTg                                                                >  2:75002/1‑76 (MQ=255)           atatACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGAt                                                              <  1:1296619/76‑1 (MQ=255)            tataCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATa                                                             >  1:1113240/1‑76 (MQ=255)            tataCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATa                                                             >  2:1098619/1‑76 (MQ=255)            tataCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATa                                                             <  2:1348495/76‑1 (MQ=255)              taCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATaga                                                           >  1:1343485/1‑76 (MQ=255)              taCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATaga                                                           <  1:374678/76‑1 (MQ=255)              taCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATaga                                                           >  2:1231753/1‑76 (MQ=255)               aCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATagag                                                          <  1:1400788/76‑1 (MQ=255)                ccAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGc                                                         >  2:1714977/1‑76 (MQ=255)                ccAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGc                                                         <  2:123570/76‑1 (MQ=255)                ccAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGc                                                         <  1:1773810/76‑1 (MQ=255)                 cAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCa                                                        >  2:1093150/1‑76 (MQ=255)                  aaaCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCaa                                                       <  2:1725655/76‑1 (MQ=255)                   aaCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAAt                                                      <  1:1202390/76‑1 (MQ=255)                   aaCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAAt                                                      >  1:662551/1‑76 (MQ=255)                    aCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATg                                                     >  1:952940/1‑76 (MQ=255)                     cGAGCCGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAAggg                                                    <  2:789241/76‑1 (MQ=255)                     cGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATgg                                                    <  2:273947/76‑1 (MQ=255)                     cGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATgg                                                    <  2:789266/76‑1 (MQ=255)                      gagaCGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGa                                                   <  1:75244/76‑1 (MQ=255)                         aCGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCg                                                <  1:1792032/76‑1 (MQ=255)                         aCGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCg                                                >  2:2079611/1‑76 (MQ=255)                           gCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCa                                              <  1:1631365/76‑1 (MQ=255)                           gCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCa                                              <  1:21777/76‑1 (MQ=255)                           gCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCa                                              <  1:784381/76‑1 (MQ=255)                           gCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCa                                              <  1:204906/76‑1 (MQ=255)                           gCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCa                                              <  2:816827/76‑1 (MQ=255)                            cACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAg                                             >  1:663209/1‑76 (MQ=255)                            cACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAg                                             >  1:852232/1‑76 (MQ=255)                              ccTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGtt                                           >  1:657455/1‑76 (MQ=255)                               cTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTa                                          >  2:521691/1‑76 (MQ=255)                               cTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTa                                          >  1:1133448/1‑76 (MQ=255)                                  tttgttGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGc                                       >  1:350248/1‑76 (MQ=255)                                   ttgttgGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCa                                      <  1:1642147/76‑1 (MQ=255)                                    tgttgGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAg                                     <  1:950310/76‑1 (MQ=255)                                     gttgggttggCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGt                                    >  2:930013/1‑76 (MQ=255)                                      ttgggttggCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTa                                   <  2:1053343/76‑1 (MQ=255)                                      ttgggttggCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTa                                   >  1:1114793/1‑76 (MQ=255)                                       tgggttggCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTAc                                  >  1:2017522/1‑76 (MQ=255)                                       tgggttggCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTAc                                  >  1:2096900/1‑76 (MQ=255)                                         ggttggCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCa                                <  1:1334922/76‑1 (MQ=255)                                         ggttggCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCa                                <  2:1310718/76‑1 (MQ=255)                                           ttggCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACt                              <  2:1113240/76‑1 (MQ=255)                                             ggCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTaa                            <  1:784653/76‑1 (MQ=255)                                              gCCATGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTaac                           >  2:958370/1‑76 (MQ=255)                                                 aTGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTaacaac                        <  2:1550603/76‑1 (MQ=255)                                                 aTGTGAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTaacaac                        >  1:1529713/1‑76 (MQ=255)                                                  tgtgAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcg                       >  2:593235/1‑76 (MQ=255)                                                   gtgAGGTAAAAAAGCCCCCTCCACCGACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcgc                      <  1:1272850/76‑1 (MQ=255)                                                    tgAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcgcg                     >  2:1805116/1‑76 (MQ=255)                                                     gAGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcgcgc                    >  2:160911/1‑76 (MQ=255)                                                      aGGTAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcgcgcg                   <  1:1504903/76‑1 (MQ=255)                                                         tAAAAAAGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAg                <  2:496628/76‑1 (MQ=255)                                                          aaaaaaGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGt               <  1:1783206/76‑1 (MQ=255)                                                          aaaaaaGCCCCCTACACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGt               <  1:1155804/76‑1 (MQ=255)                                                           aaaaaGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtg              >  2:1287516/1‑76 (MQ=255)                                                           aaaaaGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtg              <  1:614824/76‑1 (MQ=255)                                                              aaGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtgatg           <  1:1098619/76‑1 (MQ=255)                                                              aaGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtgatg           >  2:1303860/1‑76 (MQ=255)                                                              aaGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtgatg           >  2:1303874/1‑76 (MQ=255)                                                              aaGCCCCCTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtgatg           <  1:1671515/76‑1 (MQ=255)                                                                 cccccTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATgaga        >  2:1305311/1‑76 (MQ=255)                                                                  ccccTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGaa       >  2:2002846/1‑76 (MQ=255)                                                                  ccccTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGaa       >  2:1915566/1‑76 (MQ=255)                                                                  ccccTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGaa       >  2:167847/1‑76 (MQ=255)                                                                  ccccTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGaa       >  2:158702/1‑76 (MQ=255)                                                                   cccTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGaaa      <  1:1633193/76‑1 (MQ=255)                                                                    ccTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAAt     <  1:1133219/76‑1 (MQ=255)                                                                     cTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATa    <  2:522882/76‑1 (MQ=255)                                                                     cTCCACCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATa    <  1:274049/76‑1 (MQ=255)                                                                       ccaccaACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgc  >  1:301943/1‑76 (MQ=255)                                                                          |                                                                         TTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGC  >  REL606/3570203‑3570348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑