| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,928,841 | A→T | I209F (ATC→TTC) | rho → | transcription termination factor Rho |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,928,841 | 0 | A | T | 97.4% | 295.5 / ‑2.3 | 76 | I209F (ATC→TTC) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base T (35/39); total (35/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTG > REL606/3928771‑3928913 | cATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTcgac < 2:565747/76‑1 (MQ=255) aTGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTcgacg > 1:1912712/1‑76 (MQ=255) gctgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGaa > 1:965265/1‑76 (MQ=255) ctgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAAc > 2:881613/1‑76 (MQ=255) tgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACg < 2:1971261/76‑1 (MQ=255) tgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACg < 1:991212/76‑1 (MQ=255) tgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACg > 1:666893/1‑76 (MQ=255) ctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTc > 1:1120197/1‑76 (MQ=255) ctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTc < 1:1920484/76‑1 (MQ=255) gcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCg > 2:78348/1‑76 (MQ=255) aGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGga < 1:1175760/76‑1 (MQ=255) aCATTGCTCAGAGCATTGCTTACAACCCCCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGgaaga < 2:1291113/76‑1 (MQ=255) aCATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGgaaga > 1:1759557/1‑76 (MQ=255) aCATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGgaaga < 2:632559/76‑1 (MQ=255) aCATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGgaaga > 2:701769/1‑76 (MQ=255) aTTGCTCAGAGCCTTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGaagaag < 1:1873285/76‑1 (MQ=255) ttGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGt < 1:463984/76‑1 (MQ=255) ttGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGt < 1:955203/76‑1 (MQ=255) tGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTa < 2:1458878/76‑1 (MQ=255) gCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTaa < 2:1072543/76‑1 (MQ=255) ccTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTaa > 1:2101567/2‑76 (MQ=255) cTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAAc > 2:1527911/1‑76 (MQ=255) tCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAAcc < 2:84234/76‑1 (MQ=255) cAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCg > 1:2077725/1‑76 (MQ=255) cAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCg < 2:1566565/76‑1 (MQ=255) gagCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCgag < 1:1625432/76‑1 (MQ=255) agCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCgaga < 2:1912712/76‑1 (MQ=255) cATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATg > 2:303647/1‑76 (MQ=255) aTTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGc < 1:1777298/76‑1 (MQ=255) ttGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCa > 1:2099362/1‑76 (MQ=255) gCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGc > 1:884974/1‑76 (MQ=255) gCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGc < 2:965265/76‑1 (MQ=255) cTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCg > 1:453394/1‑76 (MQ=255) cTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCg < 2:1135259/76‑1 (MQ=255) tACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTc < 1:195234/76‑1 (MQ=255) cAACCCCCCGGATTGTGTGCTGATTGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTg < 1:1270746/76‑1 (MQ=255) cAACCACCCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTg < 1:445366/76‑1 (MQ=255) accaccCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGt < 2:40173/76‑1 (MQ=255) accaccCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGt < 2:307749/76‑1 (MQ=255) ccaccCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTa > 2:99342/1‑76 (MQ=255) ccaccCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTa < 2:2037200/76‑1 (MQ=255) caccCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTaa < 2:1741422/76‑1 (MQ=255) caccCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTaa < 2:545153/76‑1 (MQ=255) caccCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTaa < 2:728676/76‑1 (MQ=255) caccCGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTaa < 2:1743744/76‑1 (MQ=255) ccGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAg > 1:197659/1‑76 (MQ=255) ccGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAg < 2:1501004/76‑1 (MQ=255) ccGGATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAg > 2:787043/1‑76 (MQ=255) gATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTg > 2:1548953/1‑76 (MQ=255) gATTGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTg > 2:1021749/1‑76 (MQ=255) ttGTGTGCTTATGGTTCTGCTGATCCACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGaa < 2:1847559/76‑1 (MQ=255) ttGTGTGCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGaa < 1:665906/76‑1 (MQ=255) tgtgtgCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAg > 1:317296/1‑76 (MQ=255) tgtgtgCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAg < 1:268554/76‑1 (MQ=255) tgtgtgCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAg > 1:502833/1‑76 (MQ=255) tgtgtgCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAg > 1:15773/1‑76 (MQ=255) tgCTGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAgttgt > 2:442176/1‑76 (MQ=255) tGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGc < 2:371228/76‑1 (MQ=255) tGATGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGCGAAGTTGTTGc > 1:365284/1‑76 (MQ=255) aTGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCtt > 1:320217/1‑76 (MQ=255) aTGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCtn < 1:815438/76‑2 (MQ=255) ttgTTCTTCTGTTCGACGAACGGCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTc < 2:994786/74‑1 (MQ=255) tGGTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTc > 1:1733213/1‑76 (MQ=255) ggTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCt < 1:239477/76‑1 (MQ=255) ggTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCt < 2:838055/76‑1 (MQ=255) ggTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCt > 2:1754968/1‑76 (MQ=255) gTTCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTa > 2:181597/1‑76 (MQ=255) ttCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTAc > 2:887939/1‑76 (MQ=255) ttCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTAc > 2:966672/1‑76 (MQ=255) tCTGCTGTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTAcc > 1:1494798/1‑76 (MQ=255) ctgctgTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCt < 1:1479695/76‑1 (MQ=255) ctgctgTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCt < 1:1479683/76‑1 (MQ=255) gctgTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCttt > 2:71635/1‑76 (MQ=255) ctgTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTCCCTTTg > 2:1949667/1‑76 (MQ=255) ctgTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTg < 2:676825/76‑1 (MQ=255) ctgTTCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTg > 2:134255/1‑76 (MQ=255) | CATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTG > REL606/3928771‑3928913 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |