Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,202,355	G→A	Q69* (CAG→TAG)	iclR ←	DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,202,355	0	G	A	100.0%	177.3 / NA	46	Q69* (CAG→TAG)	iclR	DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/23);  total (23/23)

CTGCCGACCATAAAGGCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAG  >  REL606/4202282‑4202424                                                                          |                                                                      ctgcCGACCATAAAGGCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTAtt                                                                     >  2:1697417/1‑76 (MQ=255) ctgcCGACCATAAAGGCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTAtt                                                                     >  1:1818170/1‑76 (MQ=255)  tgcCGACCATAAAGGCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTg                                                                    <  1:2096125/76‑1 (MQ=255)   gcCGACCATAAAGGCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGc                                                                   >  1:1003754/1‑76 (MQ=255)    ccGACCATAAAGGCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCa                                                                  <  1:1813012/76‑1 (MQ=255)      gACCATAAAGGCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCAtt                                                                >  1:1156975/1‑76 (MQ=255)      gACCATAAAGGCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCAtt                                                                >  2:197360/1‑76 (MQ=255)       aCCATAAAGGCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATtg                                                               >  1:1413927/1‑76 (MQ=255)        ccATAAAGGCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATtgt                                                              <  1:597992/76‑1 (MQ=255)              aGGCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAg                                                        <  1:1021860/76‑1 (MQ=255)               ggCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGc                                                       <  1:1570627/76‑1 (MQ=255)               ggCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGc                                                       <  2:2069356/76‑1 (MQ=255)               ggCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGc                                                       >  1:1532718/1‑76 (MQ=255)                 cATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAg                                                     >  1:1417992/1‑76 (MQ=255)                  aTGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAgg                                                    <  2:406779/76‑1 (MQ=255)                  aTGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAgg                                                    >  1:1419905/1‑76 (MQ=255)                  aTGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAgg                                                    <  2:1296499/76‑1 (MQ=255)                  aTGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAgg                                                    <  1:866161/76‑1 (MQ=255)                  aTGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAgg                                                    <  1:501184/76‑1 (MQ=255)                     tgCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCgg                                                 >  1:857011/1‑76 (MQ=255)                     tgCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCgg                                                 <  2:1573020/76‑1 (MQ=255)                       cgcCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTg                                               <  1:605258/76‑1 (MQ=255)                          cggttGCCCAATGGCCCGGGTCGCCGGCCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGt                                            <  2:329424/73‑1 (MQ=255)                            aTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCg                                          <  1:2077661/76‑1 (MQ=255)                             ttGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGt                                         >  1:54775/1‑76 (MQ=255)                              tGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTg                                        <  1:1805202/76‑1 (MQ=255)                               gCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTgg                                       >  1:562125/1‑76 (MQ=255)                               gCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTgg                                       <  2:991884/76‑1 (MQ=255)                                  cAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAAt                                    <  2:1556586/76‑1 (MQ=255)                                     tGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTgg                                 >  1:1354394/1‑76 (MQ=255)                                     tGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTgg                                 >  1:1594902/1‑76 (MQ=255)                                        cccAGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTa                              >  2:9785/1‑76 (MQ=255)                                           aGTTCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAAcc                           >  1:1293288/1‑76 (MQ=255)                                              tCGCCGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGGGGGTCGTGGAATTGGGTAACCCgg                        >  2:1005449/1‑76 (MQ=255)                                                  cGACCTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCttg                    >  2:369677/1‑76 (MQ=255)                                                    gccTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCttgtt                  <  2:452157/75‑1 (MQ=255)                                                     ccTGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCttgttg                 >  1:1661794/1‑76 (MQ=255)                                                       tGACGCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTgcg               <  2:1155953/76‑1 (MQ=255)                                                         gcGCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGcc             <  1:1818831/75‑1 (MQ=255)                                                          cGCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCa            <  1:834450/76‑1 (MQ=255)                                                           gCACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAg           <  2:1949526/76‑1 (MQ=255)                                                            cACGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGt          >  2:1261065/1‑76 (MQ=255)                                                             aCGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGtt         >  2:62350/1‑76 (MQ=255)                                                             aCGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGtt         >  1:1157436/1‑76 (MQ=255)                                                              cGAAACCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTc        >  1:1587231/1‑76 (MQ=255)                                                                aaaCCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCg      >  1:51179/1‑76 (MQ=255)                                                                  aCCCTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTg    <  1:988027/76‑1 (MQ=255)                                                                    ccTGCTATTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAg  >  2:1319805/1‑76 (MQ=255)                                                                          |                                                                      CTGCCGACCATAAAGGCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAG  >  REL606/4202282‑4202424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑