BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,306,133	G→T	S36Y (TCC→TAC)	cls ←	cardiolipin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,306,133	0	G	T	100.0%	261.2 / NA	65	S36Y (TCC→TAC)	cls	cardiolipin synthetase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (37/28); total (37/28)

CTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGG  >  REL606/1306060‑1306205

cTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAg                                                                        <  2:624480/76‑1 (MQ=255)

cTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAg                                                                        <  2:1620524/76‑1 (MQ=255)

cTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAg                                                                        <  2:120432/76‑1 (MQ=255)

 tCGCCAACGGCAAGATAGGCAAAAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAgg                                                                       <  2:415959/76‑1 (MQ=255)

    ccAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGaa                                                                    <  2:1540439/76‑1 (MQ=255)

     cAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAAc                                                                   <  2:209020/76‑1 (MQ=255)

      aaCGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACt                                                                  <  2:442292/76‑1 (MQ=255)

      aaCGGCAAGATAGGCAACAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACt                                                                  <  1:140972/76‑1 (MQ=255)

         ggCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTgcg                                                               >  1:1346885/1‑76 (MQ=255)

         ggCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTgcg                                                               <  1:1920062/76‑1 (MQ=255)

           cAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGcgcg                                                             <  2:986287/76‑1 (MQ=255)

           cAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGcgcg                                                             >  1:1728210/1‑76 (MQ=255)

           cAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGcgcg                                                             >  1:773344/1‑76 (MQ=255)

               aTAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGcgtcgt                                                         >  1:1072143/1‑76 (MQ=255)

               aTAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGcgtcgt                                                         >  2:917339/1‑76 (MQ=255)

               aTAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGcgtcgt                                                         >  1:1692693/1‑76 (MQ=255)

                    cAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCAt                                                    >  2:572250/1‑76 (MQ=255)

                     aataatTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCAtt                                                   <  1:172546/76‑1 (MQ=255)

                       taatTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAg                                                 <  1:1884731/76‑1 (MQ=255)

                        aatTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGa                                                >  1:1087718/1‑76 (MQ=255)

                        aatTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGa                                                >  2:1725683/1‑76 (MQ=255)

                        aatTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGa                                                >  1:665676/1‑76 (MQ=255)

                        aatTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGa                                                >  1:44523/1‑76 (MQ=255)

                         atTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGaa                                               >  2:675761/1‑76 (MQ=255)

                           tCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATg                                             <  2:1222980/76‑1 (MQ=255)

                           tCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATg                                             <  2:1222997/76‑1 (MQ=255)

                           tCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATg                                             <  1:273904/76‑1 (MQ=255)

                              gACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGt                                          >  2:193439/1‑76 (MQ=255)

                              gACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGt                                          <  2:307568/76‑1 (MQ=255)

                              gACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGt                                          >  2:1121284/1‑76 (MQ=255)

                                cTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTaa                                        >  1:403302/1‑76 (MQ=255)

                                cTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTaa                                        <  1:2064783/76‑1 (MQ=255)

                                 tAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTaaa                                       <  2:1517360/76‑1 (MQ=255)

                                  aaCGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAg                                      >  2:1892858/1‑76 (MQ=255)

                                  aaCGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAg                                      >  1:86311/1‑76 (MQ=255)

                                  aaCGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAg                                      >  2:1068110/1‑76 (MQ=255)

                                      gCAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTAc                                  >  2:2080146/1‑76 (MQ=255)

                                       cAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACg                                 >  1:1021881/1‑76 (MQ=255)

                                       cAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACg                                 >  1:883930/1‑76 (MQ=255)

                                       cAGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACg                                 >  1:414389/1‑76 (MQ=255)

                                        aGAATGTAAATAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGc                                >  1:174783/1‑76 (MQ=255)

                                           aTGTAAATAATCAACAGCCACGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTg                             >  2:1461328/1‑76 (MQ=255)

                                             gTAAATAATCAACAGCCAGGCCATCGCGTAGAGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCa                           <  1:194152/76‑1 (MQ=255)

                                             gTAAATAAACAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCa                           <  2:329094/76‑1 (MQ=255)

                                               aaaTAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAAt                         >  1:146739/1‑76 (MQ=255)

                                               aaaTAATCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAAt                         >  1:1970530/1‑76 (MQ=255)

                                                 ataatCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGa                       <  1:1863011/76‑1 (MQ=255)

                                                 ataatCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGa                       >  1:1271711/1‑76 (MQ=255)

                                                  taatCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAg                      >  2:753239/1‑76 (MQ=255)

                                                  taaaCAACAGCCAGGCCATCGCGTAGGGAGCTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAg                      <  2:978957/76‑1 (MQ=255)

                                                   aatCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGc                     >  1:785901/1‑76 (MQ=255)

                                                    atCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCa                    <  1:566354/76‑1 (MQ=255)

                                                     tCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCaa                   >  2:1599960/1‑76 (MQ=255)

                                                     tCAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCaa                   <  1:901460/76‑1 (MQ=255)

                                                      cAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAAc                  <  1:29637/76‑1 (MQ=255)

                                                      cAACAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAAc                  >  2:1086449/1‑76 (MQ=255)

                                                         catccAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAg               <  2:276293/73‑1 (MQ=255)

                                                         cAGCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAg               >  1:192237/1‑76 (MQ=255)

                                                           gCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTa             <  1:2018266/76‑1 (MQ=255)

                                                           gCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTa             <  2:397541/76‑1 (MQ=255)

                                                           gCCAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTa             >  2:115137/1‑76 (MQ=255)

                                                            ccAGGCCATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTAt            >  1:213583/1‑76 (MQ=255)

                                                                 ccATCGCGTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAg       >  1:570290/1‑76 (MQ=255)

                                                                      gcgTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATgg  >  1:1542923/1‑76 (MQ=255)

                                                                      gcgTAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGAAACCAGTATCCCAGAATgg  >  2:46584/1‑76 (MQ=255)

CTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGG  >  REL606/1306060‑1306205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`